2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A079 FTAI, FTET AND AI
OCCURRENCE CURRENCE OF ESTRUS AFTER SYNCHRONIZA
ONIZATION IMPROVE
THE USE OF SEX SEMEN IN TIMED ARTIFICIAL INSEMIANTION
PROTOCOL
Pietro Sampaio Baruselli 1 , Eric K. Abe 2 , Rodrigo Vasconcellos Sala 3 , Marcílio Nichi 4 , Evanil Pires Campos Filho 5 & Manoel Francisco Sá Filho 6
1,3,4,6
UNIVERSIDADE DE SÃO PAULO, SÃO PAULO, SP, BRAZIL. 2 AGROPECUÁRIA ABE, ANANÁS, TO, BRAZIL. 5 SEXING TECHNOLOGIES, SERTÃOZINHO, SP, BRAZIL.
Previous studies have demonstrated that lactating Nelore cows displaying estrus after synchronization of ovulation protocol
have higher risk of pregnancy than those cows that did not display estrus. The objective of present study was evaluate if the occurrence of
estrus following the synchronization can improve the use of sex sorted (SX) sperm in the timed artificial insemination (TAI) protocols. A total
of 491 multiparous Nelore (Bos indicus) cows were treated with P4 device (Sincrogest ® , Ouro Fino Animal Health) and 2mg of estradiol
benzoate i.m. (BE, Sincrodiol ® , Ouro Fino Animal Health). Eight days later, the P4 devices were removed and were administrated i.m. PGF2á
(SINCROCIO ® , Ouro Fino Animal Health) and 300 IU of equine chorionic gonadotropin (eCG; Folligon ® , Intervet Shering-Plough). The
ovulation was induced using 1 mg EB i.m. 24 h after the P4 device removal. Estrus was determined by using an adhesive detection of estrous
aid (Estrotect, IVP, Spring Valley). At P4 device removal, cows received the detection of estrus aid, placed between the hips and the tail head.
Estrus was determined at TAI by the activation of each device. Immediately before the TAI cows were reassigned to receive SX (2×106 sperm
cells/ insemination dose) or CON (20x106 sperm cells/insemination dose) semen according the occurrence of estrus. Two Nelore bulls had
previously proved satisfactory pregnancy outcomes in TAI programs were used in all inseminations. The ejaculates from each bull were
equality distributed to obtain similar amount of non-sex sorted and sex sorted sperm doses. The overall occurrence of estrus was 74.7% (367/
491). There was no interaction (P = 0.87) between type semen and the occurrence of estrus on conception rate [No Estrus - CON = 43.6%,
27/62; Estrus - CON = 58.5%, 107/183; No Estrus - SX = 33.9% and 21/62; Estrus - SX = 50.0%; 92/184]. The use of SX semen (45.9%, 113/
246) resulted lower conception rate (P = 0.05) than the use of CON semen (54.7%, 134/245). In addition, cows displayed estrus had higher
(P = 0.003) conception rates (54.2%, 199/367) than those cows that did not display estrus (38.7%, 48/124).Therefore, the occurrence of
estrus after synchronization protocol improves the efficiency of TAI programs in lactating Nelore cows, regardless of the type of semen. It
is suggested that this information can be used to improve the use of sexed semen in TAI programs. [Acknowledgements: Sexing Technologies;
Ouro Fino Saúde Animal; Agropecuária Abe].
Keywords: Bos indicus, ftai, reproductive efficiency.
A080 FTAI, FTET AND AI
THE USE OF BIOABOR
ABORTOGEN
OGEN ® AND BIOLEPTOGEN
OGEN ® VACCINES CINES MAY IMPROVE REPRODUCTIVE EFFICIENCY OF BEEF COWS
Marcos Sivieri 1 , Roberta Machado Ferreira 2 , Danilo Paik Borges 3 , Thiago Silva Antonio 3 , Reuel Luiz Gonçalves 3 & Pietro Sampaio Baruselli 2
1
VETERINÁRIO AUTÔNOMO, SACRAMENTO, MG, BRAZIL. 2 FMVZ-USP, SÃO PAULO, SP, BRAZIL. 3 BIOGÊNESIS-BAGÓ, CURITIBA, PR, BRAZIL.
The aim of the present study was to evaluate the effect of the vaccines Bioabortogen ® and Bioleptogen ® on pregnancy rate (30
and 60 days after insemination) and pregnancy loss (between 30 and 60 days of pregnancy) of beef cows fixed time artificial inseminated
(FTAI). The study was conducted during the reproductive season in three commercial farms in Sacramento and Presidente Olegário cities,
state of Minas Gerais, Brazil. On random days of the estrus cycle, post partum Nelore (Bos indicus) cows (n = 268) received a progesteronereleasing
intravaginal device associated to 2 mg of estradiol benzoate. Also, blood samples were taken from the coccigea vein of a subset of
cows (n = 18) of all farms. They were used to do a serological investigation* to infectious bovine rhinotracheitis virus (IBRV), bovine viral
diarrhea virus (BVDV) and Leptospirosis. After eight days the device was removed and 150 µg of D-cloprostenol, 300 IU of eCG and 1 mg
of estradiol cypionate IM were administered. Following two more days, the cows were homogenously allocated to one of two experimental
groups (vaccinated cows and nonvaccinated control cows) and inseminated. Vaccinated cows received 5 mL of Bioabortogen ® + 5 mL of
Bioleptogen ® SC, and control cows were administered two doses of 5 mL of saline solution as placebo. Vaccine booster was given 30 days after
the first dose (30 days following FTAI). Ten days after FTAI, cows were exposed to clean-up bulls. Pregnancy rate and pregnancy loss were
analysed with PROC GLIMMIX from SAS. The serological profile revealed high infection rate for the three pathogens: 100% of positive
cows for BVD, 62% for IBR and 54% for Leptospirosis. No interations among farms were found. The 30 days pregnancy rate was greater
in vaccinated cows [42.7% (58/136) vs. 53.8% (71/132); P = 0.05]. The 60 days pregnancy rate [41.2% (56/136) vs. 51.5% (68/132); P =
0.09] and the rate of pregnancy following FTAI plus the first service of clean-up bull [62.5% (85/136) vs. 72.7% (96/132); P = 0.06] tended
to be greater in vaccinated cows compared to nonvaccinated control cows. However, pregnancy loss was unaffected by vaccination [3.5% (2/
42) vs. 4.2% (3/42); P =0.93]. The results are suggestive that it is important to adopt control procedures and prevention strategies against IBR,
BVD and Leptospirosis, including the performance of vaccination programs at farms where these pathogens have high percentage of
occurrence. [Acknowledgments: Farms Manancial, São Judas e Bela Vista].
Keywords: bovine, vaccine, reproductive deseases.
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