XII - 12th International Symposium - Digestive Physiology of Pigs

Digestive
Physiology
of Pigs
no effect of any of the tested β-glucans on net fluid and
electrolyte absorption in ETEC-infected segments. In
ETEC-infected segments PAP expression was increased.
Perfusion with any of the β-glucans did not affect PAP
expression in the intestinal mucosa. The results indicate
that β-glucans do not promote net fluid absorption in piglets
affected by post weaning diarrhea. The lack of effect of
the β-glucans in the present study may be related to the
amount and nature of β-glucans used, to the way they were
extracted or to the duration of administration.
Key words: β-glucans, ETEC, post weaning digestive
problems
2016 Impact of plant extracts on the adhesion of
enterotoxigenic Escherichia coli on the porcine intestinal
epithelial cell line IPeC-J2. A. Mader, W. Vahjen, and
J. Zentek,* Institute of Animal Nutrition, Freie Universitaet
Berlin, Berlin, Germany.
It was shown that the adherence of enterotoxigenic E. coli
strains (ETEC) to intestinal epithelial cells (IEC) is regarded
as an important initial step for colonization and infection of
piglets. The colonization with ETEC is primarily mediated
by fimbriae; one of the most common adhesins of porcine
ETEC is F4 (K88). Plant extracts may interfere with the
adherence of pathogens. The properties of water extracts
(WE) from plant byproducts of the pharmaceutical and the
food industry to reduce the binding of ETEC 147:K89:K88,
stained with CDFA-SE, to IPEC-J2 were investigated in
vitro. Cucurbita pepo L. (pulp and peel), Cynara scolymus
L. (pomace of aerial part press juice production), Daucus
carota L. (pomace of root press juice production),
Mangifera indica L. (peel), Salix alba L. (pomace of bark
ethanol extraction), and Thymus vulgaris L. (pomace of
leaf ethanol extraction) were used. The ratio of bacteria
to IPEC-J2-cells was 100:1. Cell suspension was counted
using flow cytometry, which detected attached E. coli due to
the increased fluorescence intensity of the cells. Data were
analyzed by one factorial ANOVA and posthoc Scheffé-test
with a minimum of 4 replicates per WE, WE concentration
and incubation condition. Probabilitly (P)-values