XII - 12th International Symposium - Digestive Physiology of Pigs

Digestive
Physiology
of Pigs
(1.4 g/kg BW) in trial 2. In each trial, 36 piglets were weaned
on average at 22 d of age and 6.1 kg of BW, distributed
among treatments (12 pigs/treatment) in individual pens,
and fed ad libitum a prestarter diet. During the first 6 d after
weaning, feed intake was recorded daily and all piglets
were intragastrically infused once daily at 7 p.m. with 50
mL of either water (control) or treatment solutions. On d
5 plasma was obtained from 6 pigs/treatment at −15, 0,
30, 60, and 120 min relative to infusions and on d 6 the
remaining 6 pigs/treatment were weighed, sacrificed, and
their intestines were collected for later analyses. Data
were analyzed as a mixed-effect model with pig treated
as random variable. Compared with control, CDC at 60
mg/kg BW increased (P < 0.05) mean plasma GLP-2 by
77%, small intestine length, intraepithelial lymphocytes and
cleaved caspase in the ileum; tended to increase ileum
weight and length (P < 0.08) and mean plasma GLP-1 (P
< 0.13) without affecting (P > 0.8) intake and final BW. At
120 mg/kg BW, CDC also increased (P < 0.05) GLP-1 and
GLP-2, but reduced intake by about 50% and reduced BW
as well as ileal crypt depth. Other treatments did not affect
measured parameters, except that BSE tended to depress
GLP-1 (27%) and GLP-2 (42%) compared with control. In
conclusion, oral CDC treatment potently enhanced GLP-2
secretion in weanling piglets, but the mitigation of weaninginduced
intestinal atrophy was apparently counterbalanced
by increased inflammation and reduced feed intake.
Key words: bile acids, gut growth, pigs
2033 Effect of feeding immunoglobulin (IgG) on gastrointestinal
structure in newborn pigs. J. Wolinski* 1 ,
M. Slupecka 1 , P. Ochniewicz 1 , O. Fedkiv 2 , O. Prykhodko 2 ,
G. Ushakova 3 , G. Skibo 4 , T. Kovalenko 4 , I. Osadchenko 4 ,
K. Goncharova 4 , K. Szwiec 2 , B. Weström 2 , and S. G. Pierzynowski
2,5 , 1 The Kielanowski Institute of Animal Physiology
and Nutrition, Jablonna, Poland, 2 Dept of Biology,
Lund University, Lund, Sweden, 3 Dept of Biochemistry and
Biophysics, Dnepropetrovsk National University, Dnepropetrovsk,
Ukraine, 4 Bogomoletz Institute of Physiology, Kiev,
Ukraine, 5 Dept of Medical Biology, Institute of Rural Health,
Lublin, Poland.
Colostrum ingestion leads to increased gut growth and
maturation and is an indispensable source of antibodies
(IgG) protecting the newborn pig against infection. Here
we studied the effect of feeding purified IgG on the GI tract
structure. Newborn littermate pigs were either fed colostrum
(Col), an elemental diet (ED) without or supplemented with
purified serum IgG (ED+IgG) via a stomach tube, 10 mL/
kg, during 24 h or then only ED up to 72 h where after they
were sacrificed. For morphometric studies slides were
prepared from the GI tract and stained with hematoxylin and
eosin. Feeding the ED supplemented with IgG (ED+IgG)
compared with not resulted in an increase of thickness of
stomach mucosa (from 404 to 443 μm; P < 0.0001) and
muscularis (from 1053 to 1187 μm; P < 0.05) similar to the
values observed in Col-fed piglets at 72h. The addition
with IgG had no affect on the stomach mucosal thickness
after 24h. The duodenal and jejunal morphology became
affected due IgG supplementation (ED+IgG) compared
with ED group. Interestingly, at 24h IgG addition had
XII INTERNATIONAL SYMPOSIUM ON
DIGESTIVE PHYSIOLOGY OF PIGS
107
Session IV
increased (P < 0.0001) the values of measured parameters
in duodenum (mucosa thickness from 274 to 380 μm, villi
length from 200 to 288 μm, crypt depth from 87 to 96 μm)
into those observed in col-fed piglets. Similar results were
also obtained in the jejunum with IgG treatment. However,
the effects of IgG treatment became more incoherent in
the distal direction along small intestine. In conclusion,
our results show that feeding an ED supplemented with
IgG improved the morphology of the GI tract toward that
of colostrum-fed piglets and indicates a beneficial per se
effect of IgG on the GI tract in neonatal pigs.
Key words: immunoglobulin G, colostrum, gut morphology
2034 Chenodeoxycholic acid improves intestinal permeability
in piglets. Y. van der Meer 1 , W. J. J. Gerrits* 1 ,
M. van den Bosch 2 , J. J. Holst 3 , W. Kulik 4 , and T. A. T. G.
van Kempen 5 , 1 Animal Nutrition Group, Wageningen University,
Wageningen, The Netherlands, 2 Provimi Holding
B.V., Velddriel, The Netherlands, 3 Department of Biomedical
Sciences, University of Copenhagen, Copenhagen,
Denmark, 4 Academic Medical Center, University of Amsterdam,
Amsterdam, The Netherlands, 5 North Carolina State
University, Raleigh, North Carolina, USA.
Piglets are highly susceptible to gut health-related
problems. Intravenously administered chenodeoxycholic
acid (CDCA) affects gut health mediated through GLP-2.
To test if CDCA is a suitable feed additive for improving
gut health, a trial was performed with newly weaned (21 d)
piglets offered a diet with or without 60 mg CDCA/kg feed
(n = 24/trt). Upon weaning piglets were fasted for 16 h and
then intragastrically dosed with 20 g test feed in 40 g water.
Subsequently, a jugular blood sample was taken on either
45, 90, 135, or 180 min for analysis of GLP-2, PYY, and
glucose. Afterward, piglets were fed ad libitum. On d 3.5,
7.5, and 10.5 post weaning, 8 piglets per treatment were
sacrificed for determination of in vivo intestinal permeability
using lactulose and Co-EDTA. Both markers were
administrated intragastrically and after 2h, a blood sample
was obtained through venipuncture. Immediately thereafter,
intestines were harvested, and ex vivo permeability was
measured using the everted gut sac technique with 4 kDa
FITC-dextran as marker at 25, 50, and 75% of the length of
the small intestines. Average daily feed intake, daily gain,
gain:feed, blood glucose, plasma GLP-2, and PYY were
not affected by dietary CDCA (P > 0.10). Serum Co-EDTA
and lactulose concentrations at d 10.5 tended to be lower in
CDCA pigs compared with the control pigs (P = 0.054, P =
0.089). The everted gut sac technique data did not show any
treatment effects on permeability (P > 0.10), possibly due
to reaction of the FITC-marker with light despite covering
with aluminum foil. In conclusion, CDCA tended to improve
intestinal permeability at 10.5 d post weaning when fed to
newly weaned piglets, implying that CDCA deserves further
study as a means for improving intestinal health.
Key words: chenodeoxycholic acid, gut health, piglet
2035 evaluation of immunoglobulin absorption from
colostrum supplements gavaged to newborn piglets.
J. Campbell* 1 , S. Jacobi 2 , Y. Liu 2 , K. Hard Robertson 3 , J.