<strong>Digestive</strong> <strong>Physiology</strong> <strong>of</strong> <strong>Pigs</strong> 2030 The preterm pig as a model for studying gastrointestinal development after preterm birth. R. Buddington* 1 , K. Buddington 1 , D. Black 2 , B. Hance 1 , S. Grimes 2 , S. Chattopadyhay 1 , E. Huang 2 , and P. T. Sangild 3 , 1 Department <strong>of</strong> Health and Sport Sciences, University <strong>of</strong> Memphis, Memphis, Tennessee, USA, 2 Department <strong>of</strong> Pediatrics, LeBonheur Children’s Hospital, Memphis, Tennessee, USA, 3 Department <strong>of</strong> Human Nutrition, University <strong>of</strong> Copenhagen, DK-1958 Frederiksberg, Denmark. The incidence <strong>of</strong> preterm birth in the US is 12%, is increasing, and the almost 600,000 infants born at less than 92% <strong>of</strong> term (
<strong>Digestive</strong> <strong>Physiology</strong> <strong>of</strong> <strong>Pigs</strong> (1.4 g/kg BW) in trial 2. In each trial, 36 piglets were weaned on average at 22 d <strong>of</strong> age and 6.1 kg <strong>of</strong> BW, distributed among treatments (12 pigs/treatment) in individual pens, and fed ad libitum a prestarter diet. During the first 6 d after weaning, feed intake was recorded daily and all piglets were intragastrically infused once daily at 7 p.m. with 50 mL <strong>of</strong> either water (control) or treatment solutions. On d 5 plasma was obtained from 6 pigs/treatment at −15, 0, 30, 60, and 120 min relative to infusions and on d 6 the remaining 6 pigs/treatment were weighed, sacrificed, and their intestines were collected for later analyses. Data were analyzed as a mixed-effect model with pig treated as random variable. Compared with control, CDC at 60 mg/kg BW increased (P < 0.05) mean plasma GLP-2 by 77%, small intestine length, intraepithelial lymphocytes and cleaved caspase in the ileum; tended to increase ileum weight and length (P < 0.08) and mean plasma GLP-1 (P < 0.13) without affecting (P > 0.8) intake and final BW. At 120 mg/kg BW, CDC also increased (P < 0.05) GLP-1 and GLP-2, but reduced intake by about 50% and reduced BW as well as ileal crypt depth. Other treatments did not affect measured parameters, except that BSE tended to depress GLP-1 (27%) and GLP-2 (42%) compared with control. In conclusion, oral CDC treatment potently enhanced GLP-2 secretion in weanling piglets, but the mitigation <strong>of</strong> weaninginduced intestinal atrophy was apparently counterbalanced by increased inflammation and reduced feed intake. Key words: bile acids, gut growth, pigs 2033 Effect <strong>of</strong> feeding immunoglobulin (IgG) on gastrointestinal structure in newborn pigs. J. Wolinski* 1 , M. Slupecka 1 , P. Ochniewicz 1 , O. Fedkiv 2 , O. Prykhodko 2 , G. Ushakova 3 , G. Skibo 4 , T. Kovalenko 4 , I. Osadchenko 4 , K. Goncharova 4 , K. Szwiec 2 , B. Weström 2 , and S. G. Pierzynowski 2,5 , 1 The Kielanowski Institute <strong>of</strong> Animal <strong>Physiology</strong> and Nutrition, Jablonna, Poland, 2 Dept <strong>of</strong> Biology, Lund University, Lund, Sweden, 3 Dept <strong>of</strong> Biochemistry and Biophysics, Dnepropetrovsk National University, Dnepropetrovsk, Ukraine, 4 Bogomoletz Institute <strong>of</strong> <strong>Physiology</strong>, Kiev, Ukraine, 5 Dept <strong>of</strong> Medical Biology, Institute <strong>of</strong> Rural Health, Lublin, Poland. Colostrum ingestion leads to increased gut growth and maturation and is an indispensable source <strong>of</strong> antibodies (IgG) protecting the newborn pig against infection. Here we studied the effect <strong>of</strong> feeding purified IgG on the GI tract structure. Newborn littermate pigs were either fed colostrum (Col), an elemental diet (ED) without or supplemented with purified serum IgG (ED+IgG) via a stomach tube, 10 mL/ kg, during 24 h or then only ED up to 72 h where after they were sacrificed. For morphometric studies slides were prepared from the GI tract and stained with hematoxylin and eosin. Feeding the ED supplemented with IgG (ED+IgG) compared with not resulted in an increase <strong>of</strong> thickness <strong>of</strong> stomach mucosa (from 404 to 443 μm; P < 0.0001) and muscularis (from 1053 to 1187 μm; P < 0.05) similar to the values observed in Col-fed piglets at 72h. The addition with IgG had no affect on the stomach mucosal thickness after 24h. The duodenal and jejunal morphology became affected due IgG supplementation (ED+IgG) compared with ED group. Interestingly, at 24h IgG addition had <strong>XII</strong> INTERNATIONAL SYMPOSIUM ON DIGESTIVE PHYSIOLOGY OF PIGS 107 Session IV increased (P < 0.0001) the values <strong>of</strong> measured parameters in duodenum (mucosa thickness from 274 to 380 μm, villi length from 200 to 288 μm, crypt depth from 87 to 96 μm) into those observed in col-fed piglets. Similar results were also obtained in the jejunum with IgG treatment. However, the effects <strong>of</strong> IgG treatment became more incoherent in the distal direction along small intestine. In conclusion, our results show that feeding an ED supplemented with IgG improved the morphology <strong>of</strong> the GI tract toward that <strong>of</strong> colostrum-fed piglets and indicates a beneficial per se effect <strong>of</strong> IgG on the GI tract in neonatal pigs. Key words: immunoglobulin G, colostrum, gut morphology 2034 Chenodeoxycholic acid improves intestinal permeability in piglets. Y. van der Meer 1 , W. J. J. Gerrits* 1 , M. van den Bosch 2 , J. J. Holst 3 , W. Kulik 4 , and T. A. T. G. van Kempen 5 , 1 Animal Nutrition Group, Wageningen University, Wageningen, The Netherlands, 2 Provimi Holding B.V., Velddriel, The Netherlands, 3 Department <strong>of</strong> Biomedical Sciences, University <strong>of</strong> Copenhagen, Copenhagen, Denmark, 4 Academic Medical Center, University <strong>of</strong> Amsterdam, Amsterdam, The Netherlands, 5 North Carolina State University, Raleigh, North Carolina, USA. Piglets are highly susceptible to gut health-related problems. Intravenously administered chenodeoxycholic acid (CDCA) affects gut health mediated through GLP-2. To test if CDCA is a suitable feed additive for improving gut health, a trial was performed with newly weaned (21 d) piglets <strong>of</strong>fered a diet with or without 60 mg CDCA/kg feed (n = 24/trt). Upon weaning piglets were fasted for 16 h and then intragastrically dosed with 20 g test feed in 40 g water. Subsequently, a jugular blood sample was taken on either 45, 90, 135, or 180 min for analysis <strong>of</strong> GLP-2, PYY, and glucose. Afterward, piglets were fed ad libitum. On d 3.5, 7.5, and 10.5 post weaning, 8 piglets per treatment were sacrificed for determination <strong>of</strong> in vivo intestinal permeability using lactulose and Co-EDTA. Both markers were administrated intragastrically and after 2h, a blood sample was obtained through venipuncture. Immediately thereafter, intestines were harvested, and ex vivo permeability was measured using the everted gut sac technique with 4 kDa FITC-dextran as marker at 25, 50, and 75% <strong>of</strong> the length <strong>of</strong> the small intestines. Average daily feed intake, daily gain, gain:feed, blood glucose, plasma GLP-2, and PYY were not affected by dietary CDCA (P > 0.10). Serum Co-EDTA and lactulose concentrations at d 10.5 tended to be lower in CDCA pigs compared with the control pigs (P = 0.054, P = 0.089). The everted gut sac technique data did not show any treatment effects on permeability (P > 0.10), possibly due to reaction <strong>of</strong> the FITC-marker with light despite covering with aluminum foil. In conclusion, CDCA tended to improve intestinal permeability at 10.5 d post weaning when fed to newly weaned piglets, implying that CDCA deserves further study as a means for improving intestinal health. Key words: chenodeoxycholic acid, gut health, piglet 2035 evaluation <strong>of</strong> immunoglobulin absorption from colostrum supplements gavaged to newborn piglets. J. Campbell* 1 , S. Jacobi 2 , Y. Liu 2 , K. Hard Robertson 3 , J.
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