XII - 12th International Symposium - Digestive Physiology of Pigs

Digestive
Physiology
of Pigs
g NDF - 8.36 MJ NE/kg) and a control diets (133 g NDF -
9.69 MJ NE/kg), to evaluate the impact of the selection on
the lines’ ability to cope with a digestive challenge. Seven
pairs of littermates were used in each line. Pigs were
individually housed from 10 to 15 wk of age. At 12 wk, one
pig from each pair received either the control or the high
fiber diet for 3 wk. Titanium dioxide was included in the
diet as an indigestible marker to determine the apparent
digestibility. With the control diet, ADFI was greater in
RFI+ than in RFI– pigs (1654 vs. 1336 g/d, P < 0.001) for
a similar final BW (44.75 kg for the 2 lines). With the high
fiber diet, DFI was 9% lower compared with the control
(P < 0.01) and growth rate was reduced (728 vs. 866 g/d;
P < 0.001) for RFI+ pigs. The diet did not significantly
affect the performances of RFI– pigs. There was no line
effect on digestibility coefficients, irrespective of the
digestive site and of the diet. Increasing the dietary fiber
level led to decreased digestibility coefficients of nutrients
and energy similarly for the 2 lines (58.4 vs. 70.2% for
ileal and 70.6 vs. 80.9% for fecal N digestibility for highfiber
vs. control diet; P < 0.001). To conclude, apparent
digestibility coefficient did not contribute to explain the
difference of RFI between the 2 lines, despite different
line responses on DFI and growth rate when fed the high
fiber diet. RFI– line seems to better valorize high fiber diet
than RFI+.
Key words: fiber, digestion, residual feed intake
1092 Short-term effect of dietary yeast nucleotide
supplementation on total and diurnal variation of small
intestinal enzyme activities in piglets. N. Sauer,* M.
Eklund, S. Hoerner, E. Bauer, D. Jezierny, and R. Mosenthin,
Institute of Animal Nutrition, University of Hohenheim,
Stuttgart, Germany.
A study was carried out to investigate, whether short-term
supplementation of dietary yeast nucleotides affects total
and diurnal variation of enzyme activities in the small
intestine of weaned piglets. Twelve barrows, weaned at
18 d of age (5 kg initial BW), were fitted with a simple
T-cannula at the distal ileum. Twice daily (0800 h, 2000
h), 6 piglets each received a cereal-soybean meal-based
diet with or without supplementation of 1 g/kg of a yeast
nucleotide product (Nucleoforce Piglets) in 2 consecutive
periods. In each period, digesta samples were collected
6 times at given intervals during 24 h digesta collection.
Dietary supplementation with yeast nucleotides did
not affect total enzyme activities including α-amylase,
leucine amino peptidase (LAP), maltase and lactase (P
> 0.05). Therefore, data of both treatments were pooled
to determine diurnal variations in enzyme activities. For
α-amylase, a diurnal variation in enzyme activity could be
observed (P < 0.05, Table), in addition to a trend toward
diurnal variation in maltase activity at 1330 h vs. 1630 h
(P < 0.10). There were no variations in diurnal activities
of LAP and lactase (Table). It can be concluded that yeast
nucleotides do not affect total small intestinal enzyme
activities. Independent of diet composition, α-amylase
activities may vary over time, with peak flow of α-amylase
around 6 h postprandially.
XII INTERNATIONAL SYMPOSIUM ON
DIGESTIVE PHYSIOLOGY OF PIGS
74
Session II
Table 1. Diurnal variation of enzyme activities in ileal
digesta of piglets (LSMeans; U/L)
Time point α-Amylase LAP1 Maltase Lactase
1030 h 59713bc 3974 21 39
1330 h 87203ab 6091 22A 40
1630 h 46765c 6047 20B 40
2230 h 44493c 5486 20 43
0130 h 95527a 6513 21 41
0430 h 63164bc 4167 21 40
P-value time 0.002 0.671 0.683 0.860
a,b,cLSMeans within a column with different superscripts
differ (P < 0.05).
A,BLSMeans within a column with different superscripts
tend to differ (P < 0.1).
1Leucine aminopeptidase.
Key words: enzyme activity, nucleotide, piglet
1093 Comparative effects of five phytases on the
phosphorus and calcium utilisation in the weaned piglet.
P. Guggenbuhl* 1 , Y. Wache 1 , C. Simoes-Nunes 1 , and F.
Fru 2 , 1 DSM Nutritional Products France, Research Centre
of Animal Nutrition and Health, 68305 Saint-Louis, France,
2 DSM Nutritional Products Ltd., Basel, Switzerland.
The addition of phytase to the swine diets has generally
increased phosphorus (P) digestibility and consequently
reduced fecal excretion of P. The comparative effects on
P and calcium (Ca) digestibility of the dietary inclusion of
5 different phytases were evaluated in the weaned piglet.
RONOZYME HiPhos (M) a microbial 6 phytase produced
by synthetic genes, mimicking a gene from C. braakii was
compared with the E. Coli derived phytases Phyzyme
XP10000 TPT, OptiPhos 2000 PF, MicroTech 5000 and
Quantum 2500D in 2 separate trials of 112, 28-d old weaned
piglets allocated to 8 equal groups of 14 animals each.
In the first trial they were fed throughout a 29-d period, a
vegetable-based diet without addition of mineral P (Co1(-))
or this diet supplemented with 12 g/kg of DCP (Co1(+)) or
with HiPhos 1000 U/kg (R11000) and 1500 U/kg (R11500),
Phyzyme 500 U/kg (P500) and 750 U/kg (P750) and
OptiPhos 500 U/kg (O500) and 750 U/kg (O750). The same
design was used in the second trial with the diet without
addition of mineral P (Co2(-)) or this diet supplemented with
12 g/kg of DCP (Co2(+)) or with HiPhos 1000 U/kg (R21000)
and 1500 U/kg (R21500), MicroTech 500 U/kg (M500) and
750 U/kg (M750) and Quantum 500 U/kg (Q500) and 750
U/kg (Q750). In comparison (P < 0.05) to their respective
non-supplemented groups, all phytases reduced the P
fecal concentration and P fecal excretion and significantly
increased the P digestibility and apparent P absorption. The
digestible P equivalences of R11000, R11500, P500, P750,
O500, O750, R21000, R21500, M500, M750, Q500 and
Q750 were 0.94, 1.50, 0.67, 0.92, 0.58, 1.11, 1.36, 1.55,
0.71, 1.21, 0.95 and 1.04 g of full available P/kg of feed
respectively. Ca digestibility was significantly improved and
Ca excretion significantly reduced by the phytases. The
5 phytase preparations increased the digestibility and the
apparent absorption of the P and Ca in the weaned piglet
fed a diet containing P exclusively from vegetable origin.