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XII - 12th International Symposium - Digestive Physiology of Pigs

XII - 12th International Symposium - Digestive Physiology of Pigs

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<strong>Digestive</strong><br />

<strong>Physiology</strong><br />

<strong>of</strong> <strong>Pigs</strong><br />

SBM to 87% for Glu in US2, Brazilian and Argentinean<br />

SBM. The experimental period affected SID <strong>of</strong> His, Ile and<br />

most dispensable AA, with lower SID values in period 1<br />

compared with period 2 (P < 0.05), but SID values did not<br />

differ between the SBM batches (P = 0.136 for His to P =<br />

0.975 for Glu). In conclusion, European SBM imports from<br />

Brazil, Argentina or the United States were similar in their<br />

chemical composition, and for most AA high and uniform<br />

SID values, independent from their source <strong>of</strong> origin, were<br />

obtained.<br />

Key words: standardized ileal digestibility, soybean meal,<br />

piglet<br />

1097 Comparative evaluation <strong>of</strong> standardized ileal<br />

amino acid digestibilities in protein supplements for<br />

piglets. M. Eklund* 1 , N. Sauer 1 , S. Hörner 1 , M. Rademacher<br />

2 , and R. Mosenthin 1 , 1 Institute <strong>of</strong> Animal Nutrition,<br />

Universtiy <strong>of</strong> Hohenheim, 70593 Stuttgart, Germany,<br />

2 Evonik Industries AG, 63457 Hanau-Wolfgang, Germany.<br />

The study was conducted to determine standardized ileal<br />

digestibilities (SID) <strong>of</strong> CP and AA in protein ingredients<br />

frequently used in piglet nutrition. Twelve piglets were<br />

removed from the sow at 19 d <strong>of</strong> age (6 kg initial BW), and<br />

were fitted with a simple ileal T-cannula between d 22 and<br />

23 <strong>of</strong> age. A semi-synthetic diet based on cornstarch and<br />

casein was either supplemented with soy protein concentrate<br />

(SPC), soy protein (SP), fermented soybeans (FSB),<br />

fullfat soybeans (FFSB), fluid-bed dried porcine intestinal<br />

mucosa hydrolysate (FDPIMH) or spray-dried porcine<br />

intestinal mucosa hydrolysate (SDPIMH). The contribution<br />

<strong>of</strong> CP and AA from casein and from each <strong>of</strong> the assay feed<br />

ingredients to the assay diet averaged approximately 50%.<br />

Each diet was fed to 6 animals according to a row-column<br />

design with 3 experimental periods. The SID <strong>of</strong> CP and AA<br />

were calculated by means <strong>of</strong> the difference method. The<br />

experimental period affected SID <strong>of</strong> CP and AA, with lower<br />

SID values in period 1 compared with periods 2 and 3 (P<br />

< 0.05). The SID <strong>of</strong> indispensable AA in the soy products<br />

ranged from 72% for Thr in FFSB to 92% for Arg in FSB and<br />

in SPC. The SID <strong>of</strong> CP and AA did not differ between SPC,<br />

SP, FSB and FFSB (P > 0.05). The SID <strong>of</strong> indispensable AA<br />

in the intestinal mucosa hydrolysates ranged from 53% for<br />

His in SDPIMH to 83% for Arg in DIMH. The SID <strong>of</strong> most<br />

AA did not differ between FDPIMH and SDPIMH (P > 0.05)<br />

except for SID <strong>of</strong> Arg, His, Asp, Glu and Ser which were<br />

up to 19 percentage units higher and SID <strong>of</strong> Gly which<br />

was 17 percentage units lower in SDPIMH compared with<br />

FDPIMH (P < 0.05). Compared with most SID values in the<br />

soy products, SID <strong>of</strong> CP and indispensable AA were up to<br />

19 percentage units lower in FDPIMH and SDPIMH (P <<br />

0.05). In conclusion, high and uniform SID values in the soy<br />

products indicate that the different processing procedures<br />

used to manufacture these products were efficient to<br />

inactivate antinutritional factors present in raw soybeans.<br />

For most AA in the porcine intestinal mucosa products,<br />

drying procedure did not affect SID <strong>of</strong> AA, but SID values<br />

were generally lower compared with the soy products.<br />

Key words: standardized ileal digestibility, amino acids,<br />

piglets<br />

<strong>XII</strong> INTERNATIONAL SYMPOSIUM ON<br />

DIGESTIVE PHYSIOLOGY OF PIGS<br />

76<br />

Session II<br />

1098 endogenous losses and true ileal digestibility<br />

<strong>of</strong> amino acids in piglets as influenced by feed and<br />

protein intake. M. Eklund* 1 , N. Sauer 1 , D. Jezierny 1 , M.<br />

Goerke 1 , M. Rademacher 2 , and R. Mosenthin 1 , 1 Institute <strong>of</strong><br />

Animal Nutrition, University <strong>of</strong> Hohenheim, 70593 Stuttgart,<br />

Germany, 2 Evonik Industries AG, 63457 Hanau-Wolfgang,<br />

Germany.<br />

There is evidence that with increasing protein and feed<br />

intake, ileal AA digestibilities in diets for pigs decline. This<br />

reduction may be either due to reduced true ileal digestibility<br />

(TID) and (or) increased endogenous AA secretion<br />

(IAAend). Therefore, the homoarginine method was used<br />

in the present study with piglets fed soybean meal (SBM)<br />

or casein (CAS) to determine the effect <strong>of</strong> varying feed<br />

and protein intake on TID and IAAend. A total <strong>of</strong> 51 piglets<br />

(weaned at 17 d <strong>of</strong> age, 6 kg initial BW), fitted with ileal<br />

T-cannulas, were used in 4 consecutive experiments with<br />

2 periods each. The 12 cornstarch-based diets contained<br />

6 levels <strong>of</strong> SBM or CAS (85; 135; 185; 235; 285; 335 g<br />

CP/kg, as-fed). Half <strong>of</strong> the CP source was replaced by<br />

the corresponding guanidinated CP source. Each diet<br />

was fed at 2 levels <strong>of</strong> feed intake (30; 60 g/kg BW; in<br />

total n = 4 piglets per treatment). Each period included 9<br />

d for adaptation to the diets. Ileal digesta were collected<br />

continuously for 12 h on d 10. Using the example <strong>of</strong> Lys,<br />

the TID (P < 0.05) but not IAAend (P > 0.05) decreased<br />

with increasing feed intake. Moreover, TID <strong>of</strong> Lys (P < 0.05)<br />

declined and IAAend increased (P < 0.05) with higher CP<br />

content sourced from SBM, whereas differences in CP<br />

intake sourced from CAS did not affect (P > 0.05) IAAend<br />

and TID <strong>of</strong> Lys. In conclusion, protein and feed intake can<br />

affect ileal AA digestibility measurements as influenced by<br />

the source <strong>of</strong> protein.<br />

Table 1. Effect <strong>of</strong> protein source (PS) and feeding level<br />

(FL, g/kg as-is) on IAA end and TID <strong>of</strong> Lys<br />

Protein level (PL; g/kg as-is) P-value<br />

PS FL 85 135 185 235 285 335 PS PL FL PSxPL<br />

IAA CAS 60 end,<br />

0.6 0.7 0.7 0.7 1.1 1.5

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