XII - 12th International Symposium - Digestive Physiology of Pigs

Digestive
Physiology
of Pigs
The pro-inflammatory response induced by the LPS was
suppressed by one extract of Ascophyllum nodosum which
inhibited the expression of IL8, IL6 and TNFα genes to 0.99
± 0.53 (P < 0.05), 0.75 ± 0.33 (P < 0.05) and 1.01 ± 0.17
(P < 0.05) fold, respectively. One extract of Fucus serratus
also inhibited the expression of these cytokine genes as
induced by LPS to 0.70 ± 0.32 (P < 0.01), 0.69 ± 0.38 (P <
0.05) and 1.15 ± 0.25 fold, respectively. It is concluded that
the extracts of Ascophyllum nodosum and Fucus serratus
seaweeds have potential to suppress the pro-inflammatory
response induced by the bacterial LPS in the pig colon.
Key words: anti-inflammatory, cytokine, bioactive
1032 The addition of a Bacillus licheniformis CeCT
4536 probiotic to piglet diets improves animal intestinal
microbiota and performance parameters. J. J.
Mallo* 1 , M. Oficialdegui 2 , M. I. Gracia 3 , M. Gutierrez 1 , and
P. Honrubia 1 , 1 Norel S.A., Madrid, Spain, 2 Granja Los Alecos
S.A., Navarra, Spain, 3 Imasde Agroalimentaria S.L.,
Madrid, Spain.
The trial investigated the effects of adding a new probiotic,
composed of spores of B. licheniformis strain CECT 4536
(10 9 cfu/g of product) to a standard piglet post-weaning
feeding program (C) on intestinal microbiota and growth
performance. The probiotic was added at a dosage of 1
kg/T of feed, giving a bacterial concentration of 10 6 cfu/g of
feed (T). In this trial 480 piglets were randomly housed in 16
pens and monitored from d 21 to 74 of age for differences
in performance parameters. Results were analyzed with a
PROC GLM test. The inclusion of the probiotic produced
heavier piglets; at the end of the trial, T piglets tended to
weigh more than C piglets (22.9 vs 21.7 kg; P < 0.12).
Additionally, the inclusion of B. licheniformis tended to
improve piglet growth during the trial (337 vs 313 g/d; P
< 0.12) and Feed Conversion Ratio (FCR) (1.54 vs 1.67
g feed/g gain; P < 0.12). These differences were mainly
produced in the last 2 wk of the trial, where the T animals
tended to grow more (+10.8%; 706 vs 637 g/d; P < 0.12),
and had better FCR (−20%; 1.36 vs 1.70 g feed/g gain; P <
0.05) than C animals. At the same time, samples of feces
were taken at d 0, 21 and 53 of trial to evaluate how the
probiotic affected fecal microflora. The use of the probiotic
showed higher counts of lactobacilli in feces (6.52 log10 vs
5.76 log10; P < 0.05) at d 53; with no statistical differences in
total mesophilic bacteria, coliforms, clostridia or salmonella
at d 53, and no differences at d 0 or 21. It was, therefore,
concluded that the addition of B. licheniformis CECT 4536
at a dosage of 106 cfu/g to the diet improves intestinal
microbiota balance in the piglets and promotes an easier
transition to solid feed, producing higher body weights,
better growth and improved feed conversion after weaning.
Key words: probiotic, bacillus, microflora
1033 Influence of antibiotic treatment of sows on
intestinal microbiota in their offsprings. J. Zhang* 1 , O.
Peréz 1 , J. P. Lallès 2 , and H. Smidt 1 , 1 Laboratory of Microbiology,
Wageningen University, Dreijenplein 10, 6703
HB Wageningen, the Netherlands, 2 Institut National de la
XII INTERNATIONAL SYMPOSIUM ON
DIGESTIVE PHYSIOLOGY OF PIGS
50
Session I
Recherche Agronomique, UR1341 ADNC, F-35590 Saint-
Gilles, France.
Early disturbance by antibiotic treatment on the microbial
colonization process can have drastic consequences for
gastrointestinal tract development. This study aimed at
evaluating the effects of amoxicillin treatment (AT) of sows
before and after farrowing on the intestinal microbiota in
their offspring during the suckling and the post-weaning
period. Two groups of 4 sows each were defined as control
and treatment. In the treatment, sows were treated orally
with amoxicillin (40 mg/kg BW/d) during 10 d before and
21 d after parturition. Feces of sows were collected at
the beginning and the end of the AT. Offsprings (1/sow/
time) were sacrificed at d 14, 21, 28 and 42 after birth, to
collect ileal digesta. The microbial composition of feces
and ileal digesta was analyzed by the Pig Gastrointestinal
Tract Chip. Cluster analysis of microbiota profiles of sows
showed separate grouping of samples from treated sows
at the end of AT. This was further confirmed by principal
response curves (PRC) analysis which showed a large
deviation of the treatment from the control at the end of AT
and indicated a significant effect (P = 0.045) of AT. PRC
analysis revealed a reduction of abundance of Mycoplasmalike,
L. gasseri-like, Porphyromonas asaccharolytica-like
and L. delbrueckii-like organisms of treated sows. Cluster
analysis of ileal microbiota of piglets showed samples from
d 42 grouped in a distinct assembly, while all other samples
formed, with few exceptions, 2 clusters according to
treatment, indicating AT of sows influenced the microbiota
of piglets. Microbial diversity of piglets derived from treated
sows was lower than that of controls at all time points,
and was significantly different at d 42(P = 0.018). The
PRC analysis showed deviations of the treatment from the
control at each sampling point and confirmed the sustained
AT effect on the ileal microbiota. Reduction of L. gasserilike
and Mycoplasma-like organisms of sows caused by
AT also appeared in digesta of piglets. In conclusion, pre-
and postpartum AT had a significant effect on intestinal
microbiota of sows, which in turn influenced the microbial
colonization of their offspring.
Key words: microbiota, amoxicillin, piglet
1034 Effects of yeast-dried milk (YDM) product in
creep and Phase-1 nursery diets on circulating IgA and
fecal microbiota of nursing and nursery pigs. H. Tran,*
J. W. Bundy, E. E. Hinkle, J. Walter, P. S. Miller, and T. E.
Burkey, University of Nebraska, Lincoln, NE, USA.
Four experiments were conducted to evaluate effects of
YDM in creep and phase-1 nursery diets. In Exp. 1, 24
parity-4 litters (8 litters/trt) were used. Dietary treatments
included: No creep (NC), Control creep (CTL), and
Experimental creep (EC; 10% YDM). Creep diets were fed
ad libitum from d 7 after birth until weaning. In Exp. 2, 108
weaned pigs (BW, 7.2 kg) were selected based on mean
BW of pigs from each of 3 treatments in Exp. 1 and assigned
to 1 of 18 pens (6 pens/trt). Pigs fed creep diets during Exp.
1 received the same diet during phase 1 of Exp. 2, followed
by a common diet containing antibiotics in phase 2 (d 7 to
21). Blood and fecal samples were collected at weaning,