XII - 12th International Symposium - Digestive Physiology of Pigs
XII - 12th International Symposium - Digestive Physiology of Pigs
XII - 12th International Symposium - Digestive Physiology of Pigs
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<strong>Digestive</strong><br />
<strong>Physiology</strong><br />
<strong>of</strong> <strong>Pigs</strong><br />
1110 histamine and PGe -induced chloride secretion<br />
2<br />
and the epithelial catabolism <strong>of</strong> histamine in the colon<br />
<strong>of</strong> piglets is influenced by diets high in fermentable<br />
protein. S. Kroeger* 1 , R. Pieper1 , H. G. Schwelberger3 , J.<br />
Wang2 , J. F. Richter4 , J. R. Aschenbach5 , A. G. Van Kessel2 ,<br />
and J. Zentek1 , 1Institute <strong>of</strong> Animal Nutrition, Freie Universitaet<br />
Berlin, Berlin, Germany, 2Department <strong>of</strong> Animal and<br />
Poultry Science, University <strong>of</strong> Saskatchewan, Saskatoon,<br />
Saskatchewan, Canada, 3Department Operative Medizin,<br />
Universitaetsklinik Innsbruck, Innsbruck, Austria, 4Clinical <strong>Physiology</strong>, Charité Univeritaetsmedizin, Berlin, Germany,<br />
5Veterinary <strong>Physiology</strong>, Freie Universitaet Berlin, Berlin,<br />
Germany.<br />
We studied the influence <strong>of</strong> dietary fermentable protein<br />
(fCP) and fermentable carbohydrate level (fCHO) on the<br />
epithelial response to histamine and prostaglandin E 2<br />
(PGE 2 ), as protein- or inflammation-derived secretagogues,<br />
in the colon <strong>of</strong> pigs. Furthermore, the capacity for histamine<br />
inactivation through the enzymes diamine oxidase (DAO)<br />
and histamine N-methyltransferase (HMT) was determined.<br />
Thirty-two weaned piglets were fed 4 diets for 21–23 d in<br />
a 2 × 2 factorial design, with low vs. high fCP (15 or 20%<br />
CP) and low vs. high fCHO (14.5 or 17.3% TDF). High<br />
fCP was achieved by the addition <strong>of</strong> autoclaved soybean<br />
meal (200 g/kg) and high fCHO by wheat bran and sugar<br />
beet pulp (80 or 50 g/kg). <strong>Pigs</strong> were euthanized and<br />
mucosa from the proximal colon was mounted in Ussing<br />
chambers. Histamine or PGE 2 was applied and the change<br />
<strong>of</strong> short-circuit current (ΔIsc) and tissue conductance<br />
(ΔGt) was measured. Gene expression <strong>of</strong> DAO and HMT<br />
was determined by RT-qPCR in RNA extracts from colon<br />
tissue. DAO activity was measured radiometrically and<br />
HMT activity by transmethylation <strong>of</strong> histamine. Histamine<br />
concentration in colon digesta was analyzed by ionexchange<br />
chromatography. Generalized linear model<br />
procedures in SPSS (version 19.0, Chicago, IL, USA) were<br />
used to analyze data. The ΔIsc in response to histamine<br />
was lower (P < 0.05) and ΔGt was higher (P < 0.05) for<br />
tissue taken from the high fCP groups. The ΔIsc tended to<br />
be lower after the addition <strong>of</strong> PGE 2 in groups which were<br />
fed high fCP diets. The HMT gene expression (P < 0.05)<br />
and the activities <strong>of</strong> DAO and HMT were higher (P < 0.05) in<br />
pigs receiving the high fCP diets. Histamine concentration<br />
in the colon digesta was increased by dietary fCP (P <<br />
0.05). All effects were irrespective <strong>of</strong> fCHO treatment. In<br />
conclusion, epithelial histamine and PGE 2 sensitivity and<br />
histamine inactivation through DAO and HMT are modified<br />
through diets high in fCP in weaned piglets, thus suggesting<br />
adaptation to increased histamine levels.<br />
Key words: histamine, fermentable protein, secretory<br />
response<br />
1111 Digestible energy values <strong>of</strong> feed ingredients with<br />
or without addition <strong>of</strong> enzymes complex in growing<br />
pigs. P. Cozannet* 1 , A. Preynat 1 , and J. Noblet 2 , 1 Adisseo<br />
France SAS, CERN, F-03600 Malicorne, France, 2 INRA,<br />
UMR1348 Pegase, F-35590 Saint Gilles, France.<br />
Digestible energy (DE) values and digestible nutrients<br />
content <strong>of</strong> 6 diets were measured in 60 kg male growing<br />
<strong>XII</strong> INTERNATIONAL SYMPOSIUM ON<br />
DIGESTIVE PHYSIOLOGY OF PIGS<br />
81<br />
Session II<br />
pigs fed restricted amount <strong>of</strong> feed. Diets were prepared from<br />
5 ingredients (wheat, corn, barley, wheat bran and soybean<br />
meal; inclusion levels <strong>of</strong> ingredients being independent)<br />
with or without Rovabio Excel AP (3,300 endo-ï ¢-1,4xylanase<br />
visco units and 300 endo-1,3(4)-Î 2 -glucanase<br />
units/kg <strong>of</strong> feed; 150 g/ton <strong>of</strong> feed) according a 6 × 2<br />
factorial arrangement; dietary NDF ranged from 12 to 20%<br />
<strong>of</strong> DM. <strong>Pigs</strong> (5 per treatment) were placed in metabolism<br />
cages that allowed total collections <strong>of</strong> feces and urine for<br />
10 d after a 11 d adaptation period. Samples were analyzed<br />
for GE, ash and N. Digestibilities <strong>of</strong> GE, N and organic<br />
matter (OM) and N balance were calculated. The effects<br />
<strong>of</strong> diet and enzyme were evaluated by ANOVA. In addition,<br />
the DE and digestible nutrient contents <strong>of</strong> ingredients<br />
were calculated by regression <strong>of</strong> nutritive values <strong>of</strong> diets<br />
on levels <strong>of</strong> ingredients inclusions. Apparent total tract<br />
digestibilities <strong>of</strong> OM, N and GE <strong>of</strong> diets were affected by diet<br />
characteristics (P < 0.001) in connection with a negative<br />
effect <strong>of</strong> NDF content (R = −0.97; P < 0.001) and were<br />
increased (P < 0.05) by enzyme addition (Enz) with +0.69,<br />
+1.68 and +0.76% units for OM, N and GE digestibilities,<br />
respectively. Increases in DE value due to Enz averaged<br />
0.12 MJ per kg DM (15.11 vs 14.99 MJ/kg DM; P < 0.001).<br />
The ADG (878 vs. 834 g/day; P < 0.05) and N balance<br />
were also increased by Enz addition, suggesting that it may<br />
overcome limiting amino acid contents. The calculated DE<br />
content per ingredient averaged 16.0, 16.6, 15.0, 10.6 and<br />
16.7 MJ/kg DM for wheat, corn, barley, wheat bran and<br />
soybean meal, respectively. The Enz addition increased<br />
the DE value <strong>of</strong> diets similar to the ingredients (+0.12 MJ/<br />
kg DM). Considered per ingredient, tendencies for a better<br />
response was observed for wheat and soybean meal (0.48<br />
and 1.25 MJ/kg DM, respectively). In conclusion, apparent<br />
total tract digestibility for energy <strong>of</strong> diets or raw materials<br />
can be increased by Enz addition.<br />
Key words: digestible energy, enzyme, pig<br />
1112 Effects <strong>of</strong> fibers with different fermentation characteristics<br />
on feeding motivation in adult pigs. C. Souza<br />
Da Silva* 1,2 , J. E. Bolhuis 1 , W. J. J. Gerrits 2 , B. Kemp 1 , and<br />
J. J. G. C. van den Borne 2 , 1 Adaptation <strong>Physiology</strong> Group,<br />
Wageningen University, Wageningen, The Netherlands,<br />
2 Animal Nutrition Group, Wageningen University, Wageningen,<br />
The Netherlands.<br />
Dietary fibers can be fermented in the colon, resulting in<br />
production <strong>of</strong> short–chain fatty acids (SCFA) and secretion<br />
<strong>of</strong> satiety–related peptides such as GLP–1 and PYY.<br />
Recently, we have shown that a diet containing 40% native<br />
potato starch reduced feeding motivation in adult pigs<br />
up to 7 h after feeding. The aim <strong>of</strong> the current study was<br />
to assess the effects <strong>of</strong> fibers with varying fermentation<br />
characteristics on feeding motivation in adult pigs. Sixteen<br />
pair–housed gilts (250 kg BW) received 4 diets: inulin<br />
(INU), guar gum (GG), retrograded tapioca starch (RS),<br />
and control (C), in 4 periods in a Latin square design.<br />
Each period, pigs were fed a low fiber (L) followed by a<br />
high fiber (H) diet (7 d each). Fiber levels were 7% and<br />
14% for INU, 5% and 10% for GG, 17% and 34% for RS.<br />
Fibers were exchanged for starch from the C diet based<br />
on gross energy. <strong>Pigs</strong> received 1050 g <strong>of</strong> feed twice daily.