XII - 12th International Symposium - Digestive Physiology of Pigs

Digestive
Physiology
of Pigs
d 7, 14, and 21 postweaning (pw) to evaluate serum IgA
and fecal microbiota. In Exp. 2, pigs fed CTL and EC had
greater (P = 0.10 and 0.03; respectively) IgA compared with
NC pigs. On d 7 post-weaning, pigs fed EC had greater (P
= 0.005) microbial diversity compared with CTL; however,
there was no difference in diversity indices between EC
and NC pigs. In addition, microbial similarity within group
decreased (P < 0.05) in pigs fed EC compared with other
treatments on d 7 and 21 pw. Overall (d 0 to 14), lactobacilli
16S rRNA gene copy numbers were greater (P = 0.03) in
EC (7.3 log 10 ) compared with NC pigs (6.9 log 10 ). Exp. 3 (23
parity-1 litters; 7 litters/trt) had the same treatment design
as Exp. 1. There was lower (P = 0.02) L. reuteri in the CTL
(5.1 log 10 ) compared with NC pigs (5.6 log 10 ). In Exp. 4,
108 weaned pigs (BW, 6.6 kg) were selected based on the
mean BW of all pigs from Exp. 3 and assigned to 18 pens (6
pens/trt). Exp. 4 had the same experimental design as Exp.
2 except no antibiotic was included in phase-2 diet. Pigs fed
CTL (4.8 log 10 ) and EC (4.8 log 10 ) had lower (P < 0.01) fecal
L. johnsonii compared with NC pigs (5.2 log 10 ). Lactobacilli,
L. johnsonii, and L. reuteri gene copy numbers decreased
(P < 0.001) from d 7 to d 21 postfarrowing (Exp. 3) and
increased at d 7 post-weaning (Exp. 4). Microbial ecology
and immune parameters in nursing and nursery pigs may
be affected by YDM product.
Key words: immunoglobulin A, microbiota, yeast
1035 evaluation of the growth performance and fecal
microbiota profile in developing gilts fed high-fiber
diets. W. Burger,* H. Tran, J. W. Bundy, E. E. Hinkle, R.
K. Johnson, P. S. Miller, and T. E. Burkey, University of
Nebraska, Lincoln, NE, USA.
Restricting feed and energy intake during gilt development
improves lifetime productivity and lowers breakeven selling
prices for market pigs. Inclusion of dietary insoluble fiber
XII INTERNATIONAL SYMPOSIUM ON
DIGESTIVE PHYSIOLOGY OF PIGS
51
Session I
at high concentrations is a strategy to limit energy intake
in gilts fed ad libitum. Thus, an experiment was conducted
to evaluate the effects of low energy feedstuffs on growth
performance and fecal microbial profiles in developing gilts
fed high-fiber diets in a 4-wk feeding period. Fifty-six gilts
(initial BW, 83.4 ± 2.3 kg) were housed in individual pens
and 8 pens were assigned to each of 7 treatments: 1) control
(corn-soybean meal; CTL); 2) alfalfa (ALFA); 3) beet pulp
(BEET); 4) corn bran (CBRA); 5) corn cobs (COBS); 6) rice
hulls (RIHU); and 7) soy hulls (SOHU). The experimental
diets were formulated to contain approximately 80% ME
values of the CTL diet. Pig BW were recorded (d 0, 8,
15, 21, and 28) for ADG, ADFI, and GF calculation. Fecal
samples were collected (3 pigs/treatment) coinciding with
BW measurements for microbial diversity and similarity
analyses using denaturing gradient gel electrophoresis.
Overall, decreases (P < 0.05) in BW (in gilts fed BEET
and COBS), ADG (in gilts fed ALFA, BEET, and COBS),
and ADFI (in gilts fed BEET) were observed compared
with CTL gilts. In addition, gilts fed RIHU had increased
(P < 0.05) ADFI compared with the CTL. With respect to
microbial profiles, there were no differences in microbial
similarity coefficient within group or diversity indices among
gilts fed various sources of dietary fiber on d 0, 8, and 15;
however, gilts fed ALFA (d 21), BEET (d 28), and SOHU (d
28) had greater (P < 0.05) microbial diversity (Shannon′s
and Simpson′s) compared with the CTL. Gilts fed RIHU,
COBS, and CBRA had greater (P = 0.03) similarity within
group on d 28 compared with the CTL. There was lower
(P < 0.01) bacterial similarity between CTL and SOHU
pigs on d 28 (65%) compared with d 0 (91%). Briefly, high
fiber feedstuffs affect growth performance and microbial
profiles; however, further research is needed to evaluate
how changes in gut microbes affect lifetime productivity and
health of developing gilts and their progeny.
Key words: fiber, gilt, microbial diversity