Frans_M_Everaerts_Isotachophoresis_378342.pdf

ADDITIVES TO THE ELECTROLYTES
Fig.6.39. Influence of additives on the final recording of the isotachophoretic separation of a test
mixture of anions (see Fig.6.15), carried out in the operational system at pH 6 (Table 12.1). 1 = 0.05%
Ethomene T/20; 2 = 0.05% Serdox ZCA-10; 3 = 0.05% Serdox NJAD-20; 4 = 0.05% Priminox 32;
5 = 0.05% Triton X-100; 6 = 0.1% polyvinylpyrrolidone; 7 = 0.05% Nonic 218; 8 = 0.1% Mowiol
(polyvinyi alcohol). The isotachopherogam shown in the centre represents the analysis of the test
mixture of anions after the narrow-bore tube and the detector had been rinsed well with double-
distilled water after a series of experiments carried out with Mowiol, to show the ‘memory effect’ with
this surfactant. The resolution disappears again when about ten experiments have been carried out.
were performed, many subsequent analyses could be made without the addition of
Mowiol, as Mowiol is difficult to remove. It can therefore be concluded that the adsorp-
tion of surface-active components is really important. Other workers have also reported
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