Frans_M_Everaerts_Isotachophoresis_378342.pdf

I
SEPARATION USING A THERMOCOUPLE AS DETECTOR 29 1
72 __
-1
12
i- 1
h -1
Fig.ll.7. Isotachopherogram of a test mixture of cations carried out in the operational system at pH
5.4 with water and deuterium oxide as solvents (Table 11.3). A conductivity detector (a.c. method) and
a W adsorption detector (256 nm) were applied 1 = K+; 2 = BaZ+; 3 = Na+; 4 = (CH,), N+; 5 = Pb2+;
6 = C,H,N+-CH,-CO-NH-NH, ; 7 = Tris'; 8 = histidid; 9 = creatinine*; 10 = bemidine+; 11 =
e-aminocaproic acid+; 12 = raminobutyric acid+. The amplification of both the UV absorption
detector and the conductivity detector was not changed. A = Increasing UV absorption, R = increasing
resistance; t = time.
TABLE 11.7
DIFFERENCES IN THE EFFECTIVE MOBILITIES OF SOME CATIONS IN THE SOLVENTS WATER
AND DEUTERIUM OXIDE
The values (h) refer to the step heights (mm) as measured in the linear trace of the conductivity detector.
Ionic species hHZO hDzO Ionic species hH,O hD,O
f,
K+ 0 0 Tris' 51.0 54.8
Baa+ 8.8 9.1 Histidine' 58.5 65.6
Na + 14.2 16.9 Creatinine' 65.6 78.0
Tma' 20.8 23.8 Benzidine' 81.3 98.1
Pb2+ 25.4 34.8 e-Aminocaproic acid+ 109.2 130.6
C,H,N'-CH,-CO-NH-NH, 39.4 44.8 y-Aminobutyric acid+ 115.6 149.2