Frans_M_Everaerts_Isotachophoresis_378342.pdf

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AMINO ACIDS, PEPTIDES AND PROTEINS
1
Fig.13.15. Isotachopherogram of normal serum (Fig.13.10) in an ampholyte gradient obtained with
the operational system listed in Table 13.9. The ampholyte mixture had the following composition:
pI 3.5-4: pI4-6: PI 6-8: water = I : 1.5: 0.5 : 20. In each instance 0.2 pl of ampholyte mixture was
injected and (a) 0.2 pl, (b) 0.05 pl and (c) 0 p1 of normal serum. A = Increasing UV absorption;
R = increasing resistance; t = time.
in a narrow-bore tube, hanging free in air, are plotted. However, in another system than
applied for the experiments with proteins. In the terminator zone of the operational
system in which the proteins can be separated even higher temperatures can be expected.
If too much ampholyte is added to the serum to dilute the protein zone, the resolution is
decreased.
The isotachopherograms presented in this section show that much more work must be
carried out in this field. The compositicils of the ampholyte mixtures and their