Frans_M_Everaerts_Isotachophoresis_378342.pdf

ENZYMATIC CONVERSION OF GLUCOSE (FRUCTOSE)
1
I
A
3
I’
Fig.15.2. Isotachopherogram of the reaction mixture before the enzymatic conversion. 1 = Chloride;
2 = ATP; 3 = NADP; 4 = glutamate. Various impurities from the chemicals are present, but these do
not interfere with the separation. A = Increasing UV absorption; R = increasing resistance; r = time.
Fig. 15.3 shows an isotachopherogram of the reaction mixture after only hexokinase
had been added.
The enzymatic reaction was performed with the six solutions specified in Table 15.3,
using 1.5 ml in the reaction flask each time. This flask was thermostated in a metal block
at 25"C, a heat-sink compound being applied between the flask and the metal block for
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