Frans_M_Everaerts_Isotachophoresis_378342.pdf

SEPARATION USING CONDUCTIVITY AND UV DETECTORS 345
I
4 3 2 1
Fig.14.5. Isotachopherogram of the separation of AMP, ADP and ATP in the operational system at
pH 6 (Table 12.1). An amount of 10 nmole of each component was injected. The current was stabilized at
100 PA. The time of analysis is 8 min; however, this analysis can easily be carried out in 2 min.
1 = Chloride; 2 = ATP; 3 = ADP; 4 = AMP; 5 = MES. A = Increasing W absorption; R = increasing
resistance; t = time.
In Fig.14.5, the separation of AMP, ADP and ATP in the operational system at pH 6
(Table 12.1) is shown. One can refer to Chapter 12 for operational systems that are
suitable for the conductivity detector in combination with the W absorption detector.
In Table 14.9, experimental data obtained with both the conductivity detector
(a.c. method) and the W absorption detector (256 nm) are given.
The equipment used is described in section 7.4.4.