Frans_M_Everaerts_Isotachophoresis_378342.pdf

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VALIDITY OF THE ISOTACHOPHORETIC MODEL j pHy=pllv+0.2 rp = [H+L)/2 PHV = Q c- if( IH-L~
MATHEMATICAL MODEL FOR ISOTACHOPHORESIS LIST 0010 'REG IN' 0100 01 10 0120 'REQL' PHLJCSTLI~MI~LI 9 M~HL~I~~HL,M~~LS~HPLJQHL,TITU!~J KTL I , KMI- I J KI LI J K I ML I 9 KTtn-J KML3 J K I u3 , KIMLQJ H, BH J CUL I , WUL I, MLI 1 , c ULH, csm, NF.ULU, wii 1 , QC IM , KI IL, 0125 S,(JHI QL, 0130 01 50 01 70 0172 K ULT Hb-il; ' 1VTE:GER ' 4, NL I > I I J, il, NLR , Z I 3 ZY J L t Vt Mi 'HEAL' ' AiXR4Y 'PKLI > MLI JPKD , P I , KL I , KLB, MH, MBH, Mll I , MBR CO : 10 1 , CSTn JCST I JCOI ,CBU, NEU9 ,NEU I , PH, C~HI HPI MI 1 3 M3 1 J KTB. 0173 KI~~KIR~KP~~"RJKI~I~KII~KMIJKTICO:~OI~KI~KR~ 0180 PKI JKIJPK:3rTUHEO: 10~0: 10 1; 0190 0195 0200 020 1 ' INTEGER' 'AR%4Y' NItWi,ZIN,ZFjNCO: 10 I; s : =READ ; PHL: =IIEAD;A:=HEAD;CSTLI:=FIEAD;NLI:=READ;MdLI:=HEAD; ZI : =READ; 0210 0220 'F0H'I:=l'STEP9 I'UNTIL'NLI'DB' 'RF:GIiV' PKI-I C I 1: =READ ; MLI C I 1 : =READ; ' EN!O' ; 0230 MCIHL: =READ; MHL: =READ; NLF3: =!?EAD;MOLS: =READ; 023 1 Z9 : =READ ; 02 40 0250 'FOii'I:=l'STEP'l'UNTIL'NLF3'DB' 'REG IN' PKLB C I 1 :=REAL,; Mi-V C I 1 : =READ; 'EN>' ; 0% 60 0270 0275 0280 0290 0300 'FLlil'I:=l'STEP'I'UNTIL'A'DB' 'BEGIN' NI C I 1 : =READ; MHC I 1 : =READ;M0HC I 1: =IIEAD;MBI C I 1: =REAP; ZINC 11: =ilEAD; ' FOR ' J: =1 'STEP ' 1 ' UNT IL ' NI C I 1 ' Dl3' 'REGIN'PKI C 1, J 1: =READ; MI C I, J 1 : =READ; 'END' 5 NF3 C I 1 : =READ; M09 C I 1 : =ilEAD; 0305 0310 0320 0330 ZRN C I 1 : =READ; 'FBR * J: =1 'STEP' 1 'UYTIL'N5 C I 1 ' DO ' ' BEGIN' PKl3 L: I , J 3 : =REAL); M3 C 1, J 1 : =READ; 'END' J ' END ; 0400'CBMPENT' qalculation first zone: 0410 HPL: =lo T ( -PHL) j UHL: =1 Ot ( -1 4+PHL) ; OM0 KLI t 0 1 : = 1 ; KTL I : =KMLX : =KI LI : =K I I% I : =O; 0430 0440 0450 Fig.4.13. 'FkJR'I:=l'STEP'l'UNTIL'NLI'DB' 'REGIN'KLI CI I: =KLI [I-1 1*10t( -PKLI CI l)A-IPL;QR:=ZI-I; KTLI : =KTLI +KLI C I 3; KILI : =KILI +KL I C I l*QH;
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Journal of Chromatogaphy Library -
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Journal of Chromatography Library -
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Dedicated to Pr0f.Dr.h. A.I.M. Keul
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Contents Preface ..................
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CONTENTS IX 6.4.2. The d.c. method
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CONTENTS XI 12.2.2. Applications ..
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It is very well known that charged
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Chapter 1 Historical review SUMMARY
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HISTORICAL REVIEW 3 Independently i
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THEORY
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Chapter 2 Principles of electrophor
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MOVINGBOUNDARY ELECTROPHORESIS a S
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MOVINGBOUNDARY ELECTROPHORESIS 0 iu
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ISOTACHOPHORESIS 13 2.4. PRINCIPLE
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ISOTACHOPHORESIS 15 As the anionic
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ISOTACHOPHORESIS velocities, so tha
Page 34 and 35: ISOTACHOPHORESIS 19 d.t ’.t I Fig
Page 36 and 37: ISOTACHOPHORESIS 21 t Fig.2.8. Isot
Page 38 and 39: ISOELECTRIC FOCUSING 23 The four is
Page 40 and 41: DISCUSSION 25 Fig.2.11. Survey of t
Page 42 and 43: Chapter 3 Concept of mobility SUMMA
Page 44 and 45: IONIC MOBILITY AND IONIC EQUIVALENT
Page 46 and 47: EFFECTIVE IONIC MOBILITY 31 obtain
Page 48 and 49: EFFECTIVE IONIC MOBILITY 33 compari
Page 50 and 51: EFFECTIVE IONIC MOBILITY Fig.3.4. R
Page 52 and 53: DETERMINATION OF IONIC MOBILITIES 3
Page 54 and 55: DETERMINATION OF IONIC MOBILITIES 3
Page 56 and 57: Chapter 4 Mathematical model for is
Page 58 and 59: GENERAL EQUATIONS 43 anionic specie
Page 60 and 61: GENERAL EQUATIONS 4.2.1. Equilibriu
Page 62 and 63: GENERAL EQUATIONS 41 i Similar equa
Page 64 and 65: GENERAL EQUATIONS 49 Uthzone (U-llt
Page 66 and 67: GENERAL EQUATIONS 4.2.4. Modified O
Page 68 and 69: GENERAL EQUATIONS TABLE 4.2 REDUCED
Page 70 and 71: MATHEMATICAL MODEL FOR THE STEADY S
Page 86 and 87: VALIDITY OF THE ISOTACHOPHORETIC MO
Page 92 and 93: CHECK OF THE ISOTACHOPHORETIC MODEL
Page 94 and 95: CHECK OF THE ISOTACHOPHORETIC MODEL
Page 96 and 97: REFERENCES 81 Fig.4.17. Relationshi
Page 98 and 99: Chapter 5 Choice of electrolyte sys
Page 100 and 101: CHOICE OF THE SOLVENT 85 In general
Page 102 and 103: CHOICE OF THE SOLVENT TABLE 5.2 THE
Page 104 and 105: CHOICE OF THE SOLVENT pH and pKvalu
Page 106 and 107: CHOICE OF THE SOLVENT 91 TABLE 5.4
Page 108 and 109: CHOICE OF THE pH OF THE LEADING ELE
Page 110 and 111: CHOICE OF THE pH OF THE LEADING ELE
Page 112 and 113: CHOICE OF THE TERMINATING AND LEADI
Page 114 and 115: ADDITIONS TO THE ELECTROLYTE SOLUTI
Page 116 and 117: EXAMPLES Choice of solvent. Can wat
Page 118 and 119: EXAMPLES 103 solvent, hoping that a
Page 120 and 121: EXAMPLES TABLE 5.11 STEP HEIGHTS OF
Page 122 and 123: EXAMPLES TABLE 5.12 STEP HEIGHTS OF
Page 124 and 125: Fig.5.9. Isotachopherograms of the
Page 126 and 127: EXAMPLES II 4 3 Fig.5.11. Isotachop
Page 128 and 129: REFERENCES 113 Example I. As exampl
Page 130 and 131: INSTRUMENTATION
Page 132 and 133: Chapter 6 Detection systems SUMMARY
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THERMOMETRIC RECORDING 6.1.3. Combi
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THERMOMETRIC RECORDING Potential gr
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THERMOMETRIC RECORDING /2 Fig.6.1.
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THERMOMETRIC RECORDING T t l Fig.6.
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THERMOMETRIC RECORDING 7 127 It-- I
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THERMOMETRIC RECORDING a concentrat
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HIGH-FREQUENCY CONDUCTIVITY DETECTI
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CONDUCTIVITY DETECTION 133 Fig.6.9.
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CONDUCTIVITY DETECTION 135 The d.c.
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CONDUCTIVITY DETECTION 137 Hi V 1kR
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CONDUCTIVITY DETECTION Fig.6.12. Co
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CONDUCTIVITY DETECTION Fig.6.14. Th
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CONDUCTIVITY DETECTION 143 6.4.4. T
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CONDUCTIVITY DETECTION 145 contact
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CONDUCTIVITY DETECTION 147 We can n
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CONDUCTIVITY DETECTION 149 N.Y., U.
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CONDUCTIVITY DETECTION 151 achieved
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UV ABSORPTION METER 153 -_-.- +- R
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UV ABSORPTION METER Fig.6.22. Contr
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W ABSORPTION METER r +15V -15V Mod
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W ABSORPTION METER 159 TABLE 6.4 PR
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W ABSORPTION METER 230 240 2% m zm
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to Mod (Fig 6.24) oscillator I sync
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UV ABSORPTION METER 165 dicular to
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Fig.6.31. Isotachopherograms for th
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UV ABSORPTION METER 169 50 Fig.6.33
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ADDITIVES TO THE ELECTROLYTES 171 6
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ADDITIVES TO THE ELECTROLYTES 173 e
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ADDITIVES TO THE ELECTROLYTES 175 a
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ADDITIVES TO THE ELECTROLYTES 177 o
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ADDITIVES TO THE ELECTROLYTES 179 d
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ADDITIVES TO THE ELECTROLYTES 181 T
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ADDITIVES TO THE ELECTROLYTES Fig.6
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ADDITIVES TO THE ELECTROLYTES 185 F
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ADDITIVES TO THE ELECTROLYTES 1 2 3
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ADDITIVES TO THE ELECTROLYTES 189 n
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COATING OF THE MICRO-SENSING ELECTR
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DETECTION LIMITS 193 electric drivi
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DETECTION LIMITS 195 Fig.6.48. Infl
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DETECTION LIMITS 197 TABLE 6.6 SURV
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CONCLUSION 199 average length of a
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REFERENCES 201 seen. Again it is cl
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Chapter 7 Instrumentation SUMMARY T
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INJECTION SYSTEMS I! I n Fig.7.1. P
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INJECTION SYSTEMS Fig.7.3. Exploded
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INJECTION SYSTEMS Fig.7.5. Injectio
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COUNTER ELECTRODE COMPARTMENTS 21 1
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COUNTER ELECTRODE COMPARTMENTS Even
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COUNTER ELECTRODE COMPARTMENTS 21 5
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EQUIPMENT the large bore, a more di
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EQUIPMENT 219 belonging to three cl
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EQUIPMENT 221 signal of the thermoc
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EQUIF'MENT 223 No effect on the res
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EQUIPMENT 225 Fig.7.14. Electrophor
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EQUIPMENT Fig.7.16. Photograph of t
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EQUIPMENT 229 mounted equiplanar, w
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COUNTER FLOW OF ELECTROLYTE 231 ins
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COUNTER FLOW OF ELECTROLYTE p High
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COUNTER FLOW OF ELECTROLYTE Fig.7.2
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COUNTER FLOW OF ELECTROLYTE 231 aro
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COUNTER FLOW OF ELECTROLYTE p high
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COUNTER FLOW OF ELECTROLYTE 24 1 7.
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COUNTER FLOW OF ELECTROLYTE I I 31
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COUNTER FLOW OF ELECTROLYTE max. 15
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APPLICATIONS
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Chapter 8 Introduction SUMMARY In t
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INTRODUCTION 25 1 the various ionic
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Chapter 9 Practical aspects SUMMARY
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DISTURBANCES CAUSED BY H+ AND OH 25
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DISTURBANCES CAUSED BY H+ AND OH- 2
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DISTURBANCES DUE TO CO, 263 Electro
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ENFORCED ISOTACHOPHORESIS 265 t T T
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WATER AS TERMINATOR 267 function of
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PURIFICATION OF THE TERMINATOR 7 4
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REFERENCES 271 AS an example, the c
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Chapter 10 Quantitative aspects SUM
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THERMOMETRIC MEASUREMENTS 215 vj c
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THERMOMETRIC MEASUREMENTS TABLE 10.
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CONDUCTIMETRIC MEASUREMENTS factor
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CONCLUSION 28 1 Tables 10.3-10.5 wa
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Chapter 11 Separation of cationic s
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SEPARATION USING A THERMOCOUPLE AS
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I SEPARATION USING A THERMOCOUPLE A
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SEPARATION USING CONDUCTIVITY AND U
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Chapter I2 Separation of anionic sp
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SEPARATION USING A THERMOMETRIC DET
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SEPARATION USING CONDUCTIVITY AND W
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Page 324 and 325:
Page 326 and 327:
Chapter 13 Amino acids, peptides an
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AMINO ACIDS TABLE 13.1 OPERATIONAL
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AMINO ACIDS 8- ?- 6- 5- 4- 3- 2- f-
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AMINO ACIDS 317 TABLE 13.5 STEP HEI
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AMINO ACIDS Fig.13.3. Schematic dia
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AMINO ACIDS TABLE 13.6 DETERMINATIO
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SEPARATION OF PROTEINS IN AMPHOLYTE
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Page 348 and 349:
Page 350 and 351:
SEPARATION OF SMALL PEPTIDES reprod
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Chapter I4 Separation of nucleotide
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Page 360 and 361:
Page 362 and 363:
Chapter 15 Enzymatic reactions SUMM
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ENZYMATIC CONVERSION OF GLUCOSE (FR
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ENZYMATIC CONVERSION OF PYRUVATE 35
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Chapter 16 Separations in non-aqueo
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SEPARATION OF ANIONIC SPECIES IN ME
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SEPARATION OF CATIONIC SPECIES IN M
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EXPERIMENTS IN AQUEOUS METHANOLIC S
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Chapter I7 Counter flow of electrol
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INTRODUCTION 317 In various operati
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EXPERIMENTAL 0% 10 % a b 60 % a b a
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EXPERIMENTAL 381 caused by the coun
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CONCLUSION 383 c t r ’r; I I"
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APPENDICES
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Appendix A Simplified model of movi
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PROCEDURE OF COMPUTATION A.3. PROCE
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EXPERIMENTAL 39 1 TABLE A1 THEORETI
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DISCUSSION E I I Fig.A2. Graphical
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Appendix B Diameter of the narrow-b
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Appendix C Literature 1897-1966 F.
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LITERATURE 399 B.P. Konstantinov an
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LITERATURE 401 1971 L. Arlinger, Is
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LITERATURE 403 1973 L. Arlinger and
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LITERATURE 405 A. Chrambach and J.S
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LITERATURE 407 M. Coxon and M.J. Bi
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Symbols and abbreviations SYMBOLS A
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SYMBOLS AND ABBREVIATIONS SUPERSCRI
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Subject index A Absorption meter, U
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SUBJECT INDEX 41 5 Detection limit
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SUBJECT INDEX 41 7 --_ , separation
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