Frans_M_Everaerts_Isotachophoresis_378342.pdf

II
I
7
1
7
L
h
I
c- time
QUANTITATIVE ASPECTS
Fig.lO.1. Isotachopherogram of the separation of some anions in the operational system at pH 6 (see
Table 12.1). Volumes injected: (a) 1, (b) 2 and (c) 3 p1 of a solution of 0.005N oxalate, 0.01 N
formate, 0.01 Nacetate and 0.015 NP-chloropropionate. Detection was carried out with a thermo-
metric detector.
a complete separation of a mixture of anions. It should be remembered that the smaller
the amount of ionic species introduced into the separation chamber, the shorter is the
time of analysis required for a complete separation. If the isotachopherogram in Fig. 10.1 a
should have been a chromatogram, it should have represented an incomplete separation.
Of course, normally an isotachopherogram as shown in Fig.lO.la would not have any value.
The detection limit can be decreased by using a leading electrolyte with a lower
concentration. If the concentration of the leading electrolyte is decreased to
the minimum detectable amount of an ionic species will theoretically decrease by a
N,