Frans_M_Everaerts_Isotachophoresis_378342.pdf

284 SEPARATION OF CATIONIC SPECIES IN AQUEOUS SOLUTIONS
TABLE 11.1
OPERATIONAL SYSTEM AT pH 2 SUITABLE FOR CATIONIC SEPARATIONS
Solvent: H, 0.
Electric curent &A): Ca. 50-100.
Electrolyte
Leading Terminating
Cation H+ Tns+
Concentration 0.01 N Ca. 0.01 N
Counter ion c1- c1-
PH 2 Ca. 6
Additive 0.05% Polyvinyl None
alcohol (Mowiol)*
*For experiments with a thermometric detector, this additive is not necessary.
TABLE 11.2
OPERATIONAL SYSTEM AT pH 1.9 SUITABLE FOR CATIONIC SEPARATIONS (WHIO,)
Solvent: H, 0.
Electric current &A): Ca. 50-100.
Elec tr ol y te
Leading Terminating
Cation H+ Tris+
Concentration Ca. 0.01N Ca. 0.01 N
Counter ion 10,- (0.01 N ) c1-
PH 1.5 Ca. 6
Additive 0.05% Polyvinyl None
alcohol (Mowiol)*
*For experiments with a thermometric detector, this additive is not necessary.
e.g., in Fig.ll.1 (the system WHCl) BaZ+ and F%’+ cannot be separated because they are
placed in the same circle, and CaZ+ and A13+ cannot be separated because they are
connected directly by a line, whereas Ba" and A13+ can be separated because they are
not directly connected by a line. If BaZ+, CaZ+ and A13+ are present, they form mixed
zones together. I.i+ and (C, H5)4 N’ can be separated because they are not connected
by a line. AU step heights* found in the isotachopherograrns of the experiments in
*The step height in an isotachopherogram is a qualitative measure 101 the ionic species, where the
distance between two successive peaks (the differential signal of the linear thermocouple signal)
gives all necessary quantitative information.