Frans_M_Everaerts_Isotachophoresis_378342.pdf

SEPARATION OF SMALL PEPTIDES
reproducibility are very important, because with the ampholyte mixture a constant
gradient, i.e., constant in slope and constant in length, between the leading and
terminating electrolyte zones must be maintained. This is the most important rule in
this typical version of isotachophoretic analysis. More information can be found in ref. 6.
13.3. SEPARATION OF SMALL PEP'IIDES
13.3.1. Introduction
Less attention will be paid to the separation of small peptides, because most of them
can be analyzed both in the system in which amino acids can be separated (Table 13.3)
and in the system in which the analyses with the proteins were performed (Table 13.9).
A single isotachopherogram will be presented.
Fig. 13.16. Isotachopherogram of the analysis of some small peptides obtained with the operational
system listed in Table 13.3. 1 = 5-Bromo-2,4-dihydroxybenzoic acid; 2 = chloride; 3 = glutathione;
4 = glycylglycine; 5 = glycylglycylglycylglycine; 6 = D-leucyl-L-tyrosine; 7 = L-alanine.
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