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Frans_M_Everaerts_Isotachophoresis_378342.pdf

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INJECTION SYSTEMS<br />

Fig.7.3. Exploded view of the six-way valve. 1 = Brass hexagon with screw-caps for connection of the<br />

various parts liquid-tight to the plunger housing (2), made of Kel-F; 3 = pins for locking the plunger<br />

housing in the brass hexagon (1); 4 = Arnite plunger with three pardel canals; 5 = stainless-steel<br />

screw-cap provided with a ridge for the exact determination of the position of the plunger in the<br />

plunger housing, in combination with component (6); the plunger can be switched through 60" ;<br />

7 = handle; 8 = narrow-bore tube. The clamping device of the nanowbore tube, provided with a small<br />

O-ring (see section 7.2.3.), should be noted.<br />

a bore of 1 mm. This cylinder of insulating material has two collars, and on both ends<br />

it is flat and provided with two small rubber O-rings. Again, liquid-tight connections can<br />

be made with screw-caps.<br />

In practice, the tap is very reproducible in sampling, especially when various people<br />

use the instrument. For the use of syringes, more ability is needed.<br />

It need not be explained that a shorter length of narrow-bore tube is needed for<br />

separation, because the sample is not mixed with the leading and terminating electrolytes.<br />

This six-way valve was very useful particularly when experiments were carried out in<br />

which the position of the sample is important, e.g., zone electrophoresis in narrow-bore<br />

207

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