Frans_M_Everaerts_Isotachophoresis_378342.pdf

330 AMINO ACIDS, PEPTIDES AND PROTEINS
Fig.13.11. Isotachopherogram of normal serum (Fig.13.10) in an ampholyte gradient (Fig.13.9)
obtained with the operational system listed in Table 13.9. (a) Boundary between leading and
terminating electrolytes; (b) 0.2 pl of normal serum injected; (c) 0.2 MI of normal serum and 0.2 pl
of ampholyte mixture (Fig.13.9) injected. A = Increasing W absorption;R = increasing resistance;
t = time.
electrolyte, assuming that it occupies a position between the boundary of the leading and
terminating zones. Too easy the W traces in Figs.13.11 and 13.12 will be interpreted in a
similar manner to the zone electrophoretic traces in Fig. 13.10. The differential trace from
the conductivity detector is not given because it is rather complex and does not give any
additional information.
The application of a micro-preparative instrument will indicate if the isotachophero-