Frans_M_Everaerts_Isotachophoresis_378342.pdf

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COUNTER FLOW OF ELECTROLYTE 24 1 7.5.6. Counter flow regulated by the current-stabilized power supply; the membrane Pump This method is the most accurate and simple, and is therefore discussed in more detail. The principle is shown in Fig.7.25. Fig.7.26 can be used to explain the principle of the method and also the principle by which the electric current through the narrow-bore tube (Ic) is stabilized. If through the narrow-bore tube, filed with a suitable electrolyte (leading electrolyte), an electric current is stabilized at Ic, the total voltage needed (V,) is then increasing during T I 1 Fig.7.25. Equipment for producing a counter flow of electrolyte regulated by the currentstabilized power supply. This method of pumping and also the regulation were found to be optimal in combination with the narrow-bore tube, in spite of the fact that the membrane pump does not have linear characteristics. 1 = Electronic circuit shown in Fig.7.29; 2 = set of detectors. If the counter flow of electrolyte is also to be applied for micro-preparative purposes, another means of pumping can be sought (e.g., an electroendosmotic pump).
242 INSTRUMENTATION Fig.7.26. Principle of regulation of a counter flow of electrolyte, via a membrane pump (as shown in Fig.7.25). Attention should be paid to the common terminal and the earth, which prevent disturbances to the current stabilization (electrophoretic driving current) by the counter flow regulation. This could destroy, or at least obscure, the final result. the isotachophoretic run. If gas is now produced in the electrolysis cell of the membrane pump, the volume of this electrolysis cell tends to expand. The volume can expand easily because between the electrolysis cell and a cell filled with leading electrolyte, mounted. next to it, a thin membrane (e.g., a rubber contraceptive) is mounted. This membrane is mounted with pre-stressing. In Fig.7.27, the construction of the membrane pump is shown in more detail, and a photograph is shown in Fig.7.28. The flow of liquid caused by the production of gas in the electrolysis cell of the membrane pump counteracts the increment in V,. This gas is produced by an electric current I,, in an electrolyte (e.g., 0.01 NKC1). Because V, is of the magnitude of kilovolts, V, is reduced to a value B V, with the aid of two resistors of 100 Ma and 56 ka. An electronic circuit (Fig.7.29) compares this value B V, with an adjustable V, If BlV,l< V,, (V,> 0), then I, = 0. If BlV,l> V&., then I, # 0 and the increment in V, is counter- acted. The regulation is such that I. will reach a value such that BIG1 becomes and remains approximately equal to VEf.. Thus the relationship betweenI,,, BI V,l and V, is: BI Vc I Q Vmf. then I,, = 0, and BI VCl > V,f. then I, = A (BI V, I - VXt). The optimal value for the amplification factor, dl,/dl V,l = BA, is dependent, among other factors, on the electrolytic system chosen (operational system) and on the cross- (7.4) (7.5)
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Journal of Chromatogaphy Library -
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Journal of Chromatography Library -
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Dedicated to Pr0f.Dr.h. A.I.M. Keul
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Contents Preface ..................
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CONTENTS IX 6.4.2. The d.c. method
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CONTENTS XI 12.2.2. Applications ..
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It is very well known that charged
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Chapter 1 Historical review SUMMARY
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HISTORICAL REVIEW 3 Independently i
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THEORY
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Chapter 2 Principles of electrophor
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MOVINGBOUNDARY ELECTROPHORESIS a S
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MOVINGBOUNDARY ELECTROPHORESIS 0 iu
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ISOTACHOPHORESIS 13 2.4. PRINCIPLE
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ISOTACHOPHORESIS 15 As the anionic
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ISOTACHOPHORESIS velocities, so tha
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ISOTACHOPHORESIS 19 d.t ’.t I Fig
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ISOTACHOPHORESIS 21 t Fig.2.8. Isot
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ISOELECTRIC FOCUSING 23 The four is
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DISCUSSION 25 Fig.2.11. Survey of t
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Chapter 3 Concept of mobility SUMMA
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IONIC MOBILITY AND IONIC EQUIVALENT
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EFFECTIVE IONIC MOBILITY 31 obtain
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EFFECTIVE IONIC MOBILITY 33 compari
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EFFECTIVE IONIC MOBILITY Fig.3.4. R
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DETERMINATION OF IONIC MOBILITIES 3
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DETERMINATION OF IONIC MOBILITIES 3
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Chapter 4 Mathematical model for is
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GENERAL EQUATIONS 43 anionic specie
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GENERAL EQUATIONS 4.2.1. Equilibriu
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GENERAL EQUATIONS 41 i Similar equa
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GENERAL EQUATIONS 49 Uthzone (U-llt
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GENERAL EQUATIONS 4.2.4. Modified O
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GENERAL EQUATIONS TABLE 4.2 REDUCED
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MATHEMATICAL MODEL FOR THE STEADY S
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VALIDITY OF THE ISOTACHOPHORETIC MO
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CHECK OF THE ISOTACHOPHORETIC MODEL
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CHECK OF THE ISOTACHOPHORETIC MODEL
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REFERENCES 81 Fig.4.17. Relationshi
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Chapter 5 Choice of electrolyte sys
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CHOICE OF THE SOLVENT 85 In general
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CHOICE OF THE SOLVENT TABLE 5.2 THE
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CHOICE OF THE SOLVENT pH and pKvalu
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CHOICE OF THE SOLVENT 91 TABLE 5.4
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CHOICE OF THE pH OF THE LEADING ELE
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CHOICE OF THE pH OF THE LEADING ELE
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CHOICE OF THE TERMINATING AND LEADI
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ADDITIONS TO THE ELECTROLYTE SOLUTI
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EXAMPLES Choice of solvent. Can wat
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EXAMPLES 103 solvent, hoping that a
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EXAMPLES TABLE 5.11 STEP HEIGHTS OF
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EXAMPLES TABLE 5.12 STEP HEIGHTS OF
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Fig.5.9. Isotachopherograms of the
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EXAMPLES II 4 3 Fig.5.11. Isotachop
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REFERENCES 113 Example I. As exampl
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INSTRUMENTATION
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Chapter 6 Detection systems SUMMARY
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THERMOMETRIC RECORDING 6.1.3. Combi
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THERMOMETRIC RECORDING Potential gr
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THERMOMETRIC RECORDING /2 Fig.6.1.
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THERMOMETRIC RECORDING T t l Fig.6.
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THERMOMETRIC RECORDING 7 127 It-- I
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THERMOMETRIC RECORDING a concentrat
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HIGH-FREQUENCY CONDUCTIVITY DETECTI
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CONDUCTIVITY DETECTION 133 Fig.6.9.
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CONDUCTIVITY DETECTION 135 The d.c.
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CONDUCTIVITY DETECTION 137 Hi V 1kR
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CONDUCTIVITY DETECTION Fig.6.12. Co
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CONDUCTIVITY DETECTION Fig.6.14. Th
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CONDUCTIVITY DETECTION 143 6.4.4. T
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CONDUCTIVITY DETECTION 145 contact
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CONDUCTIVITY DETECTION 147 We can n
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CONDUCTIVITY DETECTION 149 N.Y., U.
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CONDUCTIVITY DETECTION 151 achieved
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UV ABSORPTION METER 153 -_-.- +- R
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UV ABSORPTION METER Fig.6.22. Contr
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W ABSORPTION METER r +15V -15V Mod
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W ABSORPTION METER 159 TABLE 6.4 PR
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W ABSORPTION METER 230 240 2% m zm
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to Mod (Fig 6.24) oscillator I sync
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UV ABSORPTION METER 165 dicular to
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Fig.6.31. Isotachopherograms for th
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UV ABSORPTION METER 169 50 Fig.6.33
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ADDITIVES TO THE ELECTROLYTES 171 6
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ADDITIVES TO THE ELECTROLYTES 173 e
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ADDITIVES TO THE ELECTROLYTES 175 a
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ADDITIVES TO THE ELECTROLYTES 177 o
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ADDITIVES TO THE ELECTROLYTES 179 d
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ADDITIVES TO THE ELECTROLYTES 181 T
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ADDITIVES TO THE ELECTROLYTES Fig.6
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ADDITIVES TO THE ELECTROLYTES 185 F
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ADDITIVES TO THE ELECTROLYTES 1 2 3
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ADDITIVES TO THE ELECTROLYTES 189 n
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Page 208 and 209: DETECTION LIMITS 193 electric drivi
Page 210 and 211: DETECTION LIMITS 195 Fig.6.48. Infl
Page 212 and 213: DETECTION LIMITS 197 TABLE 6.6 SURV
Page 214 and 215: CONCLUSION 199 average length of a
Page 216 and 217: REFERENCES 201 seen. Again it is cl
Page 218 and 219: Chapter 7 Instrumentation SUMMARY T
Page 220 and 221: INJECTION SYSTEMS I! I n Fig.7.1. P
Page 222 and 223: INJECTION SYSTEMS Fig.7.3. Exploded
Page 224 and 225: INJECTION SYSTEMS Fig.7.5. Injectio
Page 226 and 227: COUNTER ELECTRODE COMPARTMENTS 21 1
Page 228 and 229: COUNTER ELECTRODE COMPARTMENTS Even
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Page 232 and 233: EQUIPMENT the large bore, a more di
Page 234 and 235: EQUIPMENT 219 belonging to three cl
Page 236 and 237: EQUIPMENT 221 signal of the thermoc
Page 238 and 239: EQUIF'MENT 223 No effect on the res
Page 240 and 241: EQUIPMENT 225 Fig.7.14. Electrophor
Page 242 and 243: EQUIPMENT Fig.7.16. Photograph of t
Page 244 and 245: EQUIPMENT 229 mounted equiplanar, w
Page 246 and 247: COUNTER FLOW OF ELECTROLYTE 231 ins
Page 248 and 249: COUNTER FLOW OF ELECTROLYTE p High
Page 250 and 251: COUNTER FLOW OF ELECTROLYTE Fig.7.2
Page 252 and 253: COUNTER FLOW OF ELECTROLYTE 231 aro
Page 254 and 255: COUNTER FLOW OF ELECTROLYTE p high
Page 258 and 259: COUNTER FLOW OF ELECTROLYTE I I 31
Page 260 and 261: COUNTER FLOW OF ELECTROLYTE max. 15
Page 262 and 263: APPLICATIONS
Page 264 and 265: Chapter 8 Introduction SUMMARY In t
Page 266 and 267: INTRODUCTION 25 1 the various ionic
Page 268 and 269: Chapter 9 Practical aspects SUMMARY
Page 270 and 271: DISTURBANCES CAUSED BY H+ AND OH 25
Page 274 and 275: DISTURBANCES CAUSED BY H+ AND OH- 2
Page 278 and 279: DISTURBANCES DUE TO CO, 263 Electro
Page 280 and 281: ENFORCED ISOTACHOPHORESIS 265 t T T
Page 282 and 283: WATER AS TERMINATOR 267 function of
Page 284 and 285: PURIFICATION OF THE TERMINATOR 7 4
Page 286 and 287: REFERENCES 271 AS an example, the c
Page 288 and 289: Chapter 10 Quantitative aspects SUM
Page 290 and 291: THERMOMETRIC MEASUREMENTS 215 vj c
Page 292 and 293: THERMOMETRIC MEASUREMENTS TABLE 10.
Page 294 and 295: CONDUCTIMETRIC MEASUREMENTS factor
Page 296 and 297: CONCLUSION 28 1 Tables 10.3-10.5 wa
Page 298 and 299: Chapter 11 Separation of cationic s
Page 300 and 301: SEPARATION USING A THERMOCOUPLE AS
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I SEPARATION USING A THERMOCOUPLE A
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SEPARATION USING CONDUCTIVITY AND U
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Chapter I2 Separation of anionic sp
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SEPARATION USING A THERMOMETRIC DET
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SEPARATION USING CONDUCTIVITY AND W
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Chapter 13 Amino acids, peptides an
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AMINO ACIDS TABLE 13.1 OPERATIONAL
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AMINO ACIDS 8- ?- 6- 5- 4- 3- 2- f-
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AMINO ACIDS 317 TABLE 13.5 STEP HEI
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AMINO ACIDS Fig.13.3. Schematic dia
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AMINO ACIDS TABLE 13.6 DETERMINATIO
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SEPARATION OF PROTEINS IN AMPHOLYTE
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SEPARATION OF SMALL PEPTIDES reprod
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Chapter I4 Separation of nucleotide
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Chapter 15 Enzymatic reactions SUMM
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ENZYMATIC CONVERSION OF GLUCOSE (FR
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ENZYMATIC CONVERSION OF PYRUVATE 35
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Chapter 16 Separations in non-aqueo
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SEPARATION OF ANIONIC SPECIES IN ME
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SEPARATION OF CATIONIC SPECIES IN M
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EXPERIMENTS IN AQUEOUS METHANOLIC S
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Chapter I7 Counter flow of electrol
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INTRODUCTION 317 In various operati
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EXPERIMENTAL 0% 10 % a b 60 % a b a
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EXPERIMENTAL 381 caused by the coun
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CONCLUSION 383 c t r ’r; I I"
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APPENDICES
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Appendix A Simplified model of movi
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PROCEDURE OF COMPUTATION A.3. PROCE
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EXPERIMENTAL 39 1 TABLE A1 THEORETI
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DISCUSSION E I I Fig.A2. Graphical
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Appendix B Diameter of the narrow-b
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Appendix C Literature 1897-1966 F.
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LITERATURE 399 B.P. Konstantinov an
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LITERATURE 401 1971 L. Arlinger, Is
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LITERATURE 403 1973 L. Arlinger and
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LITERATURE 405 A. Chrambach and J.S
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LITERATURE 407 M. Coxon and M.J. Bi
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Symbols and abbreviations SYMBOLS A
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SYMBOLS AND ABBREVIATIONS SUPERSCRI
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Subject index A Absorption meter, U
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SUBJECT INDEX 41 5 Detection limit
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SUBJECT INDEX 41 7 --_ , separation
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