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— 290 — 3.1.4.2. Experiment lb: Fish Artificial Propagation by Using LH-Rha + DOM The results of fish artificial propagation by using hormone of LH-RHa (mg/kg) + DOM are presented in table 4. Hormone levels Table 4 Spawning rate (%), spawning time, fecundity, fertilization (%) and hatching rate (%) o f climbing perch by using LH-RHa + DOM (3-5 mg/kg) T Spawning rate Spawning time Fecundity Fertilization rate Hatching rate °C (%) (h) (Eggs/kg fish) (%) (%) 40 pg/kg 26 7 5 ' 7:57 674,583 ■ 92.9- 99.6 ■ 50 |jg/kg 26 1 0 0 ” 7:19 663,515 ■ 94.8 ■ 9 8 .7 ' 60 Mg/kg 26 1 0 0 b 7:06 609,091 ■ 97.7 ‘ 98.5- 70 pg/kg 26 100b 6:15 725,889 b 96.7- 99.3- 80 pg/kg 26 1 0 0 b 6:12 628,875 ■ 9 6 .6 ' 99.2 ‘ (a,b) values in each column sharing same superscript are not significantly different (p > 0.05). The results in table 4 show no significant differences (p > 0.05) in fecundity, fertilization and hatching rate by using LH-RHa hormone at concentration levels from 40 to 60 mg/kg. The fecundity of fish fluctuated from 609,091 to 725,889 eggs/kg. The LH-Rha concentration of 70 mg/kg obtained the best results for artificial propagation of climbing perch. 3.1.4.3. Experiment lc: Artificial Propagation by Using Pituitary Gland of Common Carp Technical parameters of fish artificial propagation ( M ic h a & L o n g 2006) by using hormone of pituitary gland (mg/kg of fish) are presented in table 5. Hormone levels Table 5 Spawning rate (%), spawning time, fecundity, fertilization and hatching rate (%) of climbing perch by using pituitary gland of common carp T Spawning rate Spawning time Fecundity Fertilization rate Hatching rate °C (%) (h) (Eggs/kg) (%) (%) 8 mg/kg 27 1 0 0 “ 5:45 722,416 ■ 89.3 ■ 9 6 .9 ' 9 mg/kg 27 1 0 0 “ 5:45 662,689 " 79.3" 9 2 .5 ' 10 mg/kg 27 9 8 - 5:40 731,704- 93.5 ‘ 96.3 1 11 mg/kg 27 80" 6:05 676,914' 82.4 -b 93.8 ■ 12 mg/kg 27 75 b 5:50 742,045 ■ 8 6 .8 ' 9 0 .3 ' (a,b) values in each column sharing same superscript are not significantly different (p > 0.05).
— 291 — The results show no statistically significant differences (p > 0.05) in fecundity, fertilization and hatching rates between pituitary gland hormone concentration levels of 8 to 12 mg/kg. The mean fecundity fluctuated around 662,689 to 742,045 eggs/kg. The lowest fecundity and fertilization rate obtained 662,689 eggs/kg and 79.3 % respectively which were observed at the hormone concentration level of 9 mg/kg, and the highest of 731,704 eggs/kg and 93.5 % respectively were observed at concentration of 1 0 mg/kg. 3.1.4.4. Experiment 2: Nursing Climbing Perch: from Larvae to Fingerlings — Water Quality Parameters in Nursing Systems The water quality parameters in all experimental nursing systems are showed in tables 6 and 7. Treatment parameters Water temper. (°C) DO (mg/1) Parameters Table 6 Water quality parameters for the experiments in concrete tanks I II III AM PM AM PM AM PM 27.5 ± 0.8 3.12 ± 0 .5 29.5 ± 0.7 3.58 ± 0.7 28.2 ± 0.4 4.05 ± 0.5 Table 7 29.8 ± 0.4 4.05 ± 0.5 27.0 ± 0.6 4.15 ± 0.8 Water quality parameters for the experiments in earthen ponds Nursing periods (days) 29.2 ± 0.3 4.00 ± 0.7 3 10 17 24 31 38 45 Water temper. (°C) 29.0 29.5 29.5 30.0 31.0 32.0 30.0 Dis. oxygen (mg/1) 3.44 3.2 2.0 2.64 2.96 3.28 2.08 COD (ppm) 9.2 8 9.6 23.2 23.2 17.6 10 N-NH4* (ppm) 0.16 0.16 1.50 0.21 1.69 1.30 0.06 P-POj' (ppm) 0.06 0.07 0.10 0.45 0.07 0.08 0.06 During nursing periods, the water quality parameters in concrete tanks and earthen ponds such as water temperature and dissolved oxygen ranged from 27- 29.8 °C, 3.1-4.6 ppm and 29-32 °C, 2.0-3.4 ppm respectively. The concentrations of chemical oxygen demand, ammonia and phosphorus in earthen pond system fluctuated from 8-23.2 ppm, 0.06-1.69 ppm and 0.06-0.45 ppm respectively. Throughout the biological and ecological characteristics of climbing perch in the nature, most of water quality parameters were acceptable for fish growth and development in culture systems (P e k a r et al. 1998, X u a n et al. 1994, K h a n h et al. 1999).
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KONINKLIJKE ACADEMIE VOOR OVERZEESE
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