Rasterkraftmikroskopische Untersuchungen an nativen biologischen ...

ANHANG
2 Skripte für SPIDER
set mp
2
x33=54.99916
;x33 - z scale in Volts (see image file header)
x34=7.653
;x34 - sensitivity z scan in nm/V(...)
x91=0
;x91 is the first particle number (= 0 or last picked before)
x51=1
; 1st mic
x52=1
; last mic
x39=8.96
; pixel size
x92=14
; window size for particles
x27=10
;actual size of particles
x67=512
;width of micrograph in pixels
x68=512
;height of micrograph in pixels
;
; DO NOT CHANGE BELOW (unless you have to)
x31= 0.035*(x39/8.96)
; low pass filter radius
;frequency=2*pixel size/filt.rad.=const: x31=0.075*(x39/5.34)
;approxim.(particle size/2)+1)/interpolated x dimension
; or ((x92/2)+1)/x71 - corrected for better res.
x44=1
; compression of the mic for peak search, eher unnötig da Bilder klein!
x79=INT(x27/x44)
;x79 - actual size of a particle divided by x44=width of peaks
;for pixel size = 4.78, was 52/4
;act. for all mics the best value is 13 (52/4) (?)
; Range of CCC in center step.
x27=int(x27/x44)+1
x93 = 1
;x93: number of slices per image
do lb51 x55=x51,x52
;micrograph number x55
;convert images in ascii format to spider images
cp from ascii
raw{***x55}.dat
N
x67,x68
1
ou{***x55}
;mirror at x axis
mr
ou{***x55}
out{***x55}
X
;p_picneu:
x99=0
fi x23,x24
out{***x55}
(12,2)
x73=INT(x92/2)
x63=INT(x92/x44)+1
;window size/x44
;subtract constant plane of image minimum value (to get positive file values) and
;scale AFM raw data in nm: converted binary * hardscale[V/LSB] * softscale[nm/V] :
fs x35,x36
out{***x55}
ar
out{***x55}
_1
135