N-(1,3-Dimethylbutyl)-N

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OECD SIDS N-(1,3-DIMETHYLBUTYL)-N´-PHENYL-1,4-PHENYLENEDIAMINE 3. ENVIRONMENTAL FATE AND PATHWAYS ID: 793-24-8 DATE: 11.05.2005 26 49 (282) [24] 28 61 (299) [26] Test condition : Manometric test measuring oxygen consumption Reliability : (2) valid with restrictions Study in accordance with generally accepted scientific standards and described in sufficient detail Flag : Critical study for SIDS endpoint 03.11.2004 (42) Type : Aerobic Inoculum : other: acclimated bacterial seed Concentration : 30 mg/l related to Test substance related to Contact time : Degradation : = 7.2 (±) % after 32 day(s) Result : Deg. product : Method : other: Shake-flask method comparable to Gledhill method listed in U.S.E.P.A. 40 CFR Ch 1 subpart D paragraph 796.3100. Year : 1979 GLP : No Test substance : other TS: Santoflex 13 Method : Monsanto Shake Flask Procedure The shake flask system is similar to that described by Gledhill (1975)[Appl. Microbiol. 30: 922]. In the shake flask procedure, 60 ml of acclimated bacterial seed is mixed with 440 ml of minimal salts media in fluted 2-liter Erlenmeyer flasks. A weighed quantity (approximately 15 mg) of the appropriate test material is added to each flask except for the control. After aerating the solution with 70 % oxygen in nitrogen, an open reservoir containing 10 ml of 0.2 N barium hydroxide is suspended via a glass tube inserted in a rubber stopper. Provisions for removal and addition of the barium hydroxide solution, aeration and sampling are provided. After sealing, the flasks are agitated on a rotary shaker at 80 rpm in the dark at ambient temperature. Periodic removal (e.g. 3, 7, 14, 21, 28 and 32 d) and titration of the barium hydroxide solution are used to determine the CO2 evolved. Fresh barium hydroxide solution is added back at each sampling point. CO2 evolution values obtained with the control are subtracted from values for test material. Remark : It is not described how acclimated bacterial seed was obtained Result : Degradation related to CO2 evolution; considering the rapid primary degradation, the failure to obtain significant CO2 evolution suggests that metabolites are formed which are less rapidly degraded than 6PPD Primary degradation was checked with aerated water, and a rapid 6PPD decline (60 % in 25 h) of a 1 mg/l solution was observed Test condition : Analyses for Santoflex 13 involved extraction with methylene chloride followed by concentration and gas chromatography using a Hewlett- Packard 5710A or 5730A chromatograph equipped with dual flame ionization detectors. Samples were injected directly into a 3 mm ID glass column Reliability : (4) not assignable Documentation insufficient for assessment Flag : Critical study for SIDS endpoint 08.08.2003 (36) Type : Aerobic Inoculum : other: Mississippi River water Concentration : 1 mg/l related to Test substance related to Contact time : 70 UNEP PUBLICATIONS
OECD SIDS N-(1,3-DIMETHYLBUTYL)-N´-PHENYL-1,4-PHENYLENEDIAMINE 3. ENVIRONMENTAL FATE AND PATHWAYS ID: 793-24-8 DATE: 11.05.2005 Degradation : 97 (±) % after 22 hour(s) Result : Kinetic of testsubst. : 1 hour(s) 40 % 2 hour(s) 57 % 3 hour(s) 67 % 5 hour(s) 74 % 22 hour(s) 97 % Deg. product : Method : other: River die-away in Mississippi River water Year : 1981 GLP : Yes Test substance : other TS: Santoflex 13 Method : Aqueous Die-Away Screening Method The die-away screening method involved exposure of the test chemical to three types of aqueous environments: - purified (Milli-Q) water - membrane filtered Mississippi River water and - glass-wool filtered Mississippi River water. The die-away (= decrease in concentration) of the test chemical was monitored as a function of time by an appropriate analytical method (see TC). The Mississippi River water was collected on 1981-04-27 at the St. Louis waterfront (Eads Bridge). A portion of the water was sterilized by membrane filtration through 0.2 µm filters (Gelman Metricel GA-8, 47 mm). A second portion was filtered through glass wool to remove large inert particulates, but retaining the active biomass. Purified water for the test was obtained from a Milli-Q Water Purification System. 500 mL of each water were added to 32-ounce Boston round bottles. 20 µL of a 0.6263 g/25 mL stock solution in dimethyl sulfoxide was injected with a 25 µL syringe into each bottle resulting in a nominal S-13 concentration of 1002 µg/L. After mixing, a 10 mL aliquot of each water was removed for zero time analyses. The bottles were then sealed with TFE-fluorocarbon lined caps and stored in the dark at ambient temperature (24 °C). At appropriate sampling points, a 10 mL aliquot of each water was removed from each bottle for analysis. Remark : Degrees of transformation not determined; adsorption onto suspended matter may have affected the observed results Result : Rate of disappearance of 6PPD in biologically active and sterile Mississippi River water and in deionized water are listed respectively as follows (% remainder of about 1 mg/l initial concentration): Hour Water active sterile deionized 0 hour 100 % 100 % 100 % 1 hour 60 % 85 % 100 % 2 hour 43 % 70 % 88 % 3 hour 33 % 56 % 86 % 4 hour 38 % 49 % 80 % 5 hour 26 % 41 % 65 % 22 hour 3 % 4 % 12 % The estimated half-lives due to primary transformation are 2.9 h in biologically active river water, 3.9 h in sterile river water, and 6.8 h in sterile deionized water. Test condition : Analytical Method: Santoflex 13 analyses involved extraction of 10 mL aqueous samples with 2 mL ethyl acetate followed by measurement of the S-13 in the extract by gas chromatography using a nitrogen/phosphorus selective detector. The method was validated in Mississippi River water in the concentration range 100 to 1000 µg/L (ppb) Reliability : (2) valid with restrictions Study in accordance with generally accepted scientific standards and described in sufficient detail UNEP PUBLICATIONS 71
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N-(1,3-Dimethylbutyl)-N

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