N-(1,3-Dimethylbutyl)-N

OECD SIDS
N-(1,3-DIMETHYLBUTYL)-N´-PHENYL-1,4-PHENYLENEDIAMINE
4. ECOTOXICITY ID: 793-24-8
DATE: 11.05.2005
Dilution water source: Tap water after dechlorinated by passing through
activated carbon filter. Dilution water chemistry: Hardness: 41 mg/L as
CaCO3; pH: 7.0. Stock and test solution and how they are prepared: Stock
solution was prepared daily. Each test solution was prepared by diluting 20
mg/l solution containing 0.02 % HCO-50, prepared daily with diluting water
by using a continuous diluting apparatus. Concentrations dosing rate, flowthrough
rate, in what medium: Concentrations of the test solutions were 0,
0.15, 0.22, 0.34, 0.51, 0.76, and 1.14 mg/l (main test) and 0.07 and 0.10
mg/l (supplemental test) because fatality was more than 50 % observed at
0.15 mg/l in the main run. Vehicle/solvent and concentrations: HCO-50 was
used. Control: dilution water control and dispersant control (11.4 mg/l) were
run. Stability of the test chemical solutions: unstable, however, transparent,
no precipitate and colour formed during 24-hour monitoring period.
Exposure vessel type: 3 l glass beaker. Number of replicates, fish per
replicate: 1, 10 individuals/replicate. Water chemistry in test (O2, pH) in the
control and one concentration where effects were observed: Dissolved
oxygen readings and pH values were taken daily during 96-hour exposure
period. Dissolved oxygen concentration: 7.1 - 8.6 mg/l. pH values: 6.7 - 7.1.
Test temperature: Water temperature at 23.1 - 23.8 °C during 96-hour
exposure period.
Method of calculating mean measured concentrations: Geometric mean
because the measured concentrations were less than± 20 % of the nominal
concentration.
Statistical methods: The LC50 (Moving average) was calculated by using
TOXDAT Multi Method Program (US EPA) and probit by EcoTox-Statics
(ver 1.1,Oita University)
Test substance : N-(1,3-Dimethylbutyl)-N-phenyl-1,4-phenylendiamine (CAS No. 793 - 24 -
8). Source: Synthesized by the Toray Research Center, Lot No. 99S271C,
Purity = 91.0 %. Chemical structure and stability of the chemical during use
were confirmed by NMR, IR and HPLC. Kept at room temperature in a dark
place until use.
Conclusion : LC50 for medaka was determined to be 0.028 mg/L (confident limit: 0.020 -
0.038 mg/L) for 96 hours based on geometric means of measured
concentrations.
Reliability : (1) valid without restriction
Reliable without restriction
Experimental design and analytical procedure were well documented
Flag : Critical study for SIDS endpoint
08.08.2003 (46)
Type : flow through
Species : Pimephales promelas (Fish, fresh water)
Exposure period : 28 day(s)
Unit : mg/l
LC50 : = .15 calculated
Limit test :
Analytical monitoring : Yes
Method : other: Methods for Acute Toxicity Tests with Fish, Macroinvertebrates and
Amphibians (US-EPA 1975)
Year : 1979
GLP : Yes
Test substance : other TS: Santoflex 13
Method : Flow-through system; proportional diluter; exchange rate: 14 times / 24 h;
aerated well water at 22 °C (+/- 2 °C), carrier: acetone; monitoring of TS by
GC/FID-analysis.
Remark : Several aspects of the study have to be clarified
- Ammonia: On page 7 of the study it is reported that the well water
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