N-(1,3-Dimethylbutyl)-N

OECD SIDS
N-(1,3-DIMETHYLBUTYL)-N´-PHENYL-1,4-PHENYLENEDIAMINE
4. ECOTOXICITY ID: 793-24-8
DATE: 11.05.2005
*: No observation was made because all animals were dead at this
observation time. (Mortality: %)
Statistical results as appropriate: Calculated EiC50 values for Daphnia
magna exposed to the test chemical based on measured concentrations
under Flow-Through test conditions
Expos.period EiC50 95%confid.lim. Statistical method
(hour) (mg/l) (mg/l)
24 0.40 0.06-1.23 Binominal
48 0.23 0.17-0.31 Binominal
Observation of No Observed Effect Concentrations (NOECi) and Lowest
Concentration in 100 % mortality or immobility values
Expos. period NOECi Lowest Concentration
(hour) (mg/l) in 100 % mort./immob. values (mg/l)
24 0.06 1.23
48 0.05 1.23
Biological observations: Was control response satisfactory: Yes.
Cumulative numbers of dead or immobilized Daphnia during observation
period. 0-1 (Dispersant control) (mortality: 0-5 %)
Test condition : Analytical procedures: Yes. Test solutions were measured using by
HPLC before and after 24-hour interval.
Analytical monitoring: By HPLC analysis, 14.5-51.0 % of the nominal
concentration at preparation; 4.8-71.5 % after 24 hours exposure period.
Test organisms:
. source, supplier, any pre-treatment, breeding method: Supplied by
NIES (Japan).
· Age at study initiation: Juveniles within 24h old.
· Control group: Yes.
Test conditions:
· Stock solution was prepared daily. Each test solution was prepared
by diluting 10 mg/L solution containing 0.005 % HCO-50, prepared daily
with diluting mineral medium (Elendt M4) by using a continuous diluting
apparatus.
· Concentrations dosing rate, flow-through rate, in what medium:
Concentrations of the test solutions were 0, 0.11, 0.19, 0.34, 0.62,
1.11 and 2.00 mg/l.
. Test temperature range: 20±1 °C (19.8-20.3 °C).
· Exposure vessel type: 400 mL test solution in a 500 ml glass
container; 2 containers per dose.
· Dilution water source: Elendt M4.
· Dilution water chemistry: Hardness: 253 mg/l as CaCO3.
· Lighting: Room light, 16h: 8h light-darkness cycle.
. Water chemistry in test: DO= 8.3 - 8.9 mg/l, > 60 % of saturation
(8.84 mg/l at 20 °C); pH=7.7-8.0.
· Feeding: Chlorella vulgaris, 0.1 - 0.2 mgC/day/individual (breeding).
No feeding during test period.
Element (unit) basis: Cumulative numbers of died immobilized juveniles.
Test design: Number of replicates = 2; individuals per replicate = 10;
Concentrations: 0, 0.11, 0.19, 0.34, 0.62, 1.11 and 2.00 mg/l.
Method of calculating mean measured concentrations: Mathematical mean.
Unit: mg/l (calculated based on the nominal concentrations).
Test substance : N-(1,3-Dimethylbutyl)-N-phenyl-1,4-phenylendiamine (CAS No. 793 - 24 -
8). Source: Synthesized by the Toray Research Center, Lot No. 99S271C,
Purity = 91.0 %. Chemical structure and stability of the chemical during use
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