N-(1,3-Dimethylbutyl)-N

OECD SIDS
N-(1,3-DIMETHYLBUTYL)-N´-PHENYL-1,4-PHENYLENEDIAMINE
3. ENVIRONMENTAL FATE AND PATHWAYS ID: 793-24-8
DATE: 11.05.2005
Degradation : 97 (±) % after 22 hour(s)
Result :
Kinetic of testsubst. : 1 hour(s) 40 %
2 hour(s) 57 %
3 hour(s) 67 %
5 hour(s) 74 %
22 hour(s) 97 %
Deg. product :
Method : other: River die-away in Mississippi River water
Year : 1981
GLP : Yes
Test substance : other TS: Santoflex 13
Method : Aqueous Die-Away Screening Method
The die-away screening method involved exposure of the test chemical to
three types of aqueous environments:
- purified (Milli-Q) water
- membrane filtered Mississippi River water and
- glass-wool filtered Mississippi River water.
The die-away (= decrease in concentration) of the test chemical was
monitored as a function of time by an appropriate analytical method (see
TC).
The Mississippi River water was collected on 1981-04-27 at the St. Louis
waterfront (Eads Bridge). A portion of the water was sterilized by
membrane filtration through 0.2 µm filters (Gelman Metricel GA-8, 47 mm).
A second portion was filtered through glass wool to remove large inert
particulates, but retaining the active biomass. Purified water for the test
was obtained from a Milli-Q Water Purification System.
500 mL of each water were added to 32-ounce Boston round bottles. 20 µL
of a 0.6263 g/25 mL stock solution in dimethyl sulfoxide was injected with a
25 µL syringe into each bottle resulting in a nominal S-13 concentration of
1002 µg/L. After mixing, a 10 mL aliquot of each water was removed for
zero time analyses. The bottles were then sealed with TFE-fluorocarbon
lined caps and stored in the dark at ambient temperature (24 °C). At
appropriate sampling points, a 10 mL aliquot of each water was removed
from each bottle for analysis.
Remark : Degrees of transformation not determined; adsorption onto
suspended matter may have affected the observed results
Result : Rate of disappearance of 6PPD in biologically active and sterile Mississippi
River water and in deionized water are listed respectively as follows (%
remainder of about 1 mg/l initial concentration):
Hour Water
active sterile deionized
0 hour 100 % 100 % 100 %
1 hour 60 % 85 % 100 %
2 hour 43 % 70 % 88 %
3 hour 33 % 56 % 86 %
4 hour 38 % 49 % 80 %
5 hour 26 % 41 % 65 %
22 hour 3 % 4 % 12 %
The estimated half-lives due to primary transformation are
2.9 h in biologically active river water, 3.9 h in sterile
river water, and 6.8 h in sterile deionized water.
Test condition : Analytical Method: Santoflex 13 analyses involved extraction of 10 mL
aqueous samples with 2 mL ethyl acetate followed by measurement of the
S-13 in the extract by gas chromatography using a nitrogen/phosphorus
selective detector. The method was validated in Mississippi River water in
the concentration range 100 to 1000 µg/L (ppb)
Reliability : (2) valid with restrictions
Study in accordance with generally accepted scientific
standards and described in sufficient detail
UNEP PUBLICATIONS 71