N-(1,3-Dimethylbutyl)-N

OECD SIDS
N-(1,3-DIMETHYLBUTYL)-N´-PHENYL-1,4-PHENYLENEDIAMINE
4. ECOTOXICITY ID: 793-24-8
DATE: 11.05.2005
96 0.6 0.2 - 2
cell number 96 0.6 0.2 - 2
Confidence interval 96h EC50 = 0.2 - 2 mg/l
24 h EC50 = 2 mg/l
48 h EC50 = 0.5 mg/l
No effect in solvent control after 96 h
Results of 96 h exposure. Percentage change is increase or decrease of in
vivo chlorophyll a as compared to control at 24, 48, 72 and 96 h and
increase or decrease of cell numbers in exposed cultures as compared to
control at 96 h.
Percentage change
conc. pH Chlorophyll a Cell no.
(mg/l; ppm) 0 h 96 h 24 h 48 h 72 h 96 h 96 h
control 7.8 8.3 --- --- --- --- ---
sol. control 7.8 8.3 0 0 +1 +3 +3
0.1 8.0 8.4 -2 -16 -10 -7 -7
0.3 8.0 8.4 -5 -36 -27 -18 -13
0.6 8.1 8.1 -23 -55 -51 -40 -49
1 8.4 8.7 -31 -69 -72 -66 -66
3 8.9 8.5 -72 -84 -94 -98 -97
The cell numbers were decresed by 7 % at 0.1 mg/l and by 13 % at 0.3
mg/l after 96 h. Using the mean, a 96 h EC10 of 0.2 mg/l was obtained,
which may be used as a 96 h NOEC
Test condition : - Test substance: Dark-gray pellets, test solution prepared in acetone,
0.05 ml acetone added to 125 ml test flask at maximun
- Test organism: Selenastrum capricornutum, culture from U.S. EPA
Environmental Research Laboratory, Corvallis, Oregon
- Initial inoculum: ~ 20000 cells/ml
- Temperature: 24 +/-1 °C
- Illumination: 4000 lux, "cool" white light
- Incubations: triplicate cultures for each test concentration and control, 96
h range-finding test
- Measurement of chlorophyll a: Turner modell lll fluorometer
- Cell count: hemacytometer and Zeiss Standard 14 compound microscope
- Statistical analysis: "Student's" t-test (Steel and Torrie, 1960) confidence
level: 95 % (p