N-(1,3-Dimethylbutyl)-N

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OECD SIDS N-(1,3-DIMETHYLBUTYL)-N´-PHENYL-1,4-PHENYLENEDIAMINE 4. ECOTOXICITY ID: 793-24-8 DATE: 11.05.2005 14 0.883 21 0.673 28 1.10 Mean 0.92 Chemical characteristics of dilution water: Parameter: Concentration (mg/l), Method Alkalinity (CaCO3): 360, Titrimetric (Hach Kit) Hardness (CaCO3): 250, Titrimetric (Hach Kit) NO3-NO2-N: 0.21, Colorimetric (Technicon Auto Analyzer I) Total NH3: < 0.05, NH3 Probe (Extech Model 8002-8) Calcium: 24.5, Flame Atomic Absorpltion (Perkin-Elmer model 305-B) Iron: < 0.04, Furnace Atomic Absorption (Perkin-Elmer Model 2100) Copper: < 0.04, Furnace Atomic Absorption Zinc: < 0.02, Flame Atomic Absorption Cadmium: 0.008, Furnace Atomic Absorption Cobalt: < 0.002, Furnace Atomic Absorption Arsenic: < 0.01, Furnace Atomic Absorption Lead: < 0.002, Furnace Atomic Absorption Aluminum: < 0.1, Flame Atomic Absorption Nickel: 0.005, Flame Atomic Absorption Dissolved Oxygen: 9.3, D.O. Probe (Extech Model 8012) Statistical method: LC50 values and 95 % confidence interval were determined by using the method described by Litchfield and Wilcoxon (1949). Reliability : (3) invalid Significant methodological deficiencies Flag : Critical study for SIDS endpoint 08.11.2004 (47) Type : Static Species : Brachydanio rerio (Fish, fresh water) Exposure period : 96 hour(s) Unit : mg/l LC0 : 5 LC100 : 100 Limit test : Analytical monitoring : No Method : other: see remarks Year : 1984 GLP : No Test substance : other TS: Vulkanox 4020 Remark : Following OECD 203 The powdered test substance was dispersed in water. LCvalues given above are nominal concentrations: weight of the dispersed substance per liter water. Although the solubility of 6PPD is in the range of 1 mg/l, it is reported that a solution containing 1 g/l was prepared by direct addition of 6PPD into the test solution. Reliability : (4) not assignable Test protocol not available 12.05.2003 (48) Type : Static Species : Lepomis macrochirus (Fish, fresh water) Exposure period : 96 hour(s) Unit : mg/l LC50 : = .4 Limit test : 80 UNEP PUBLICATIONS
OECD SIDS N-(1,3-DIMETHYLBUTYL)-N´-PHENYL-1,4-PHENYLENEDIAMINE 4. ECOTOXICITY ID: 793-24-8 DATE: 11.05.2005 Analytical monitoring : No Method : other Year : 1976 GLP : No Test substance : other TS: Santoflex 13 Method : Monsanto Standard Protocol Oct. 1976 Remark : Test substance concentration not monitored during exposure; due to the instability of the TS in aqueous solution, the effective TS concentrations are expected to be lower than the applied nominal concentrations. Very low oxygen concentration at the end of incubation Result : Confidence interval for 96 h LC50 = 0.32 - 0.50 mg/l 24 h LC50 = 0.65 mg/l (0.41 - 1.0 mg/l) 48 h LC50 = 0.45 mg/l (0.31 - 0.65 mg/l) Test condition : - Carrier: acetone - 15 l water in all glass vessels, 10 fish per vessel - Fish length 3.8 cm - Fish not fed 48 h prior to exposure, nor during exposure - No aeration oxygen ranged from 8.6 mg/l (98 % saturation) to 0.2 mg/l (2 % saturation) at the beginning and end of exposure, respectively - Temperature 22 +/- 1 °C - pH ranged from 7.2 initially to 6.7 at the end of the test - Observations and mortality counts were made every 24 h following initiation of exposure - Statistics: least squares regression analysis to calculate LC 50 and 95 % confidence intervals Test substance : Santoflex (KDO3-017) CP-22423, dark red-brown colored Reliability : (3) invalid Significant methodological deficiencies 04.11.2004 (49) Type : Static Species : Salmo gairdneri (Fish, estuary, fresh water) Exposure period : 96 hour(s) Unit : mg/l LC50 : = .14 Limit test : Analytical monitoring : No Method : other Year : 1976 GLP : No Test substance : other TS: Santoflex 13 Method : Monsanto Standard Protocol Oct. 1976 Remark : Test substance concentration not monitored during exposure; due to the instability of the TS in aqueous solution, the effective TS concentrations are expected to be lower than the applied nominal concentrations Result : Confidence interval for 96 h LC50 = 0.12 - 0.16 mg/l 24 h LC50 = 0.28 mg/l (0.21 - 0.36 mg/l) 48 h LC50 = 0.18 mg/l (0.16 - 0.20 mg/l) Test condition : - Carrier: acetone - 15 l water in all glass vessels, 10 fish per vessel - Fish length 3.7 cm - Fish not fed 48 h prior to exposure, nor during exposure - No aeration oxygen ranged from 9.0 mg/l (93% saturation) to 2.8 mg/l (26 % of saturation) at the beginning and end of exposure, respectively - Temperature 12 +/- 1 °C - pH ranged from 7.0 initially to 6.8 at the end of the test - Observations and mortality counts were made every 24 h following UNEP PUBLICATIONS 81
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N-(1,3-Dimethylbutyl)-N

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