N-(1,3-Dimethylbutyl)-N
N-(1,3-Dimethylbutyl)-N
N-(1,3-Dimethylbutyl)-N
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OECD SIDS<br />
N-(1,3-DIMETHYLBUTYL)-N´-PHENYL-1,4-PHENYLENEDIAMINE<br />
4. ECOTOXICITY ID: 793-24-8<br />
DATE: 11.05.2005<br />
4.1 ACUTE/PROLONGED TOXICITY TO FISH<br />
Type : flow through<br />
Species : Oryzias latipes (Fish, fresh water)<br />
Exposure period : 96 hour(s)<br />
Unit : mg/l<br />
Limit test :<br />
Analytical monitoring : Yes<br />
Method : OECD Guide-line 203 "Fish, Acute Toxicity Test"<br />
Year : 2001<br />
GLP : Yes<br />
Test substance : other TS: Purity > 91 %<br />
Result : · Nominal concentrations: (main test): 0, 0.15, 0.22, 0.34,<br />
0.51, 0.76, and 1.14 mg/l (main test)<br />
(supplemental test): 0.07 and<br />
0.10 mg/l<br />
· Measured concentrations : (Main test)<br />
Nom.conc.(mg/l) Meas.conc.(mg/l) Percent of nominal<br />
0 hour 24 hour Geom. 0 hour 24 hour<br />
mean<br />
Control (0) < 0.04 < 0.04 --- --- ---<br />
Disper.Con.(0) < 0.04 < 0.04 --- --- ---<br />
0.15 0.05 0.05 0.05 33.3 33.3<br />
0.22 0.06 0.08 0.07 27.3 36.4<br />
0.34 0.09 0.14 0.11 26.5 41.2<br />
0.51 0.11 0.17 0.14 21.6 33.3<br />
0.76 0.21 0.32 0.26 27.6 42.1<br />
1.14 0.36 0.53 0.44 31.6 46.5<br />
(Supplemental test)<br />
Control (0) < 0.006 < 0.006 --- --- ---<br />
Disper.Con.(0) < 0.006 < 0.006 --- --- ---<br />
0.07 0.009 0.007 0.008 12.9 10.0<br />
0.10 0.017 0.012 0.014 17.0 12.0<br />
· Unit : mg/l.<br />
· Element value: Cumulative numbers of died or stressed<br />
fish<br />
· Statistical results as appropriate: Not applied.<br />
Percent mortality of Oryzias latipes exposed to the test chemical<br />
(Main test)<br />
Meas.conc.(mg/l) Cumul. number of died fish (% mortality)<br />
24 hour 48 hour 72 hour 96 hour<br />
Control (0) 0 (0) 0 (0) 0 (0) 0 (0)<br />
Disper.Con.(0) 0 (0) 0 (0) 0 (0) 0 (0)<br />
0.05 0 (0) 2 (20) 3 (30) 7 (70)<br />
0.07 0 (0) 2 (20) 5 (50) 10(100)<br />
0.11 0 (0) 9 (90) 10(100) -*<br />
0.14 4 (40) 10(100) -* -*<br />
0.26 9 (90) 10(100) -* -<br />
0.44 10(100) -* -* -*<br />
*: No observation was made because all fish were dead at this<br />
observation time.<br />
(Supplemental test)<br />
Meas.conc.(mg/l) Cumul. number of died fish (% mortality)<br />
24 hour 48 hour 72 hour 96 hour<br />
Control (0) 0 (0) 0 (0) 0 (0) 0 (0)<br />
UNEP PUBLICATIONS 75