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N-(1,3-Dimethylbutyl)-N

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OECD SIDS<br />

N-(1,3-DIMETHYLBUTYL)-N´-PHENYL-1,4-PHENYLENEDIAMINE<br />

4. ECOTOXICITY ID: 793-24-8<br />

DATE: 11.05.2005<br />

4.1 ACUTE/PROLONGED TOXICITY TO FISH<br />

Type : flow through<br />

Species : Oryzias latipes (Fish, fresh water)<br />

Exposure period : 96 hour(s)<br />

Unit : mg/l<br />

Limit test :<br />

Analytical monitoring : Yes<br />

Method : OECD Guide-line 203 "Fish, Acute Toxicity Test"<br />

Year : 2001<br />

GLP : Yes<br />

Test substance : other TS: Purity > 91 %<br />

Result : · Nominal concentrations: (main test): 0, 0.15, 0.22, 0.34,<br />

0.51, 0.76, and 1.14 mg/l (main test)<br />

(supplemental test): 0.07 and<br />

0.10 mg/l<br />

· Measured concentrations : (Main test)<br />

Nom.conc.(mg/l) Meas.conc.(mg/l) Percent of nominal<br />

0 hour 24 hour Geom. 0 hour 24 hour<br />

mean<br />

Control (0) < 0.04 < 0.04 --- --- ---<br />

Disper.Con.(0) < 0.04 < 0.04 --- --- ---<br />

0.15 0.05 0.05 0.05 33.3 33.3<br />

0.22 0.06 0.08 0.07 27.3 36.4<br />

0.34 0.09 0.14 0.11 26.5 41.2<br />

0.51 0.11 0.17 0.14 21.6 33.3<br />

0.76 0.21 0.32 0.26 27.6 42.1<br />

1.14 0.36 0.53 0.44 31.6 46.5<br />

(Supplemental test)<br />

Control (0) < 0.006 < 0.006 --- --- ---<br />

Disper.Con.(0) < 0.006 < 0.006 --- --- ---<br />

0.07 0.009 0.007 0.008 12.9 10.0<br />

0.10 0.017 0.012 0.014 17.0 12.0<br />

· Unit : mg/l.<br />

· Element value: Cumulative numbers of died or stressed<br />

fish<br />

· Statistical results as appropriate: Not applied.<br />

Percent mortality of Oryzias latipes exposed to the test chemical<br />

(Main test)<br />

Meas.conc.(mg/l) Cumul. number of died fish (% mortality)<br />

24 hour 48 hour 72 hour 96 hour<br />

Control (0) 0 (0) 0 (0) 0 (0) 0 (0)<br />

Disper.Con.(0) 0 (0) 0 (0) 0 (0) 0 (0)<br />

0.05 0 (0) 2 (20) 3 (30) 7 (70)<br />

0.07 0 (0) 2 (20) 5 (50) 10(100)<br />

0.11 0 (0) 9 (90) 10(100) -*<br />

0.14 4 (40) 10(100) -* -*<br />

0.26 9 (90) 10(100) -* -<br />

0.44 10(100) -* -* -*<br />

*: No observation was made because all fish were dead at this<br />

observation time.<br />

(Supplemental test)<br />

Meas.conc.(mg/l) Cumul. number of died fish (% mortality)<br />

24 hour 48 hour 72 hour 96 hour<br />

Control (0) 0 (0) 0 (0) 0 (0) 0 (0)<br />

UNEP PUBLICATIONS 75

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