N-(1,3-Dimethylbutyl)-N

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OECD SIDS N-(1,3-DIMETHYLBUTYL)-N´-PHENYL-1,4-PHENYLENEDIAMINE 4. ECOTOXICITY ID: 793-24-8 DATE: 11.05.2005 Disper.Con.(0) 0 (0) 0 (0) 0 (0) 0 (0) 0.008 0 (0) 0 (0) 0 (0) 0 (0) 0.014 0 (0) 0 (0) 1 (10) 10 (10) - Lowest test substance concentration causing 100% mortality: 0.07 mg/l at 96 h test period. - Mortality of controls: No mortality observed during the test period. - Abnormal responses: Abnormal swimming behaviour observed. Symtoms of toxicity observed in Oryzias latipes exposed to the test chemical under flow-through test conditions (Main test) Meas.conc.(mg/l) Symtoms 24 hour 48 hour 72 hour 96 hour Control (0) 0 (0) 0 (0) 0 (0) 0 (0) Disper.Con.(0) 0 (0) 0 (0) 0 (0) 0 (0) 0.05 0 (0) B (1) B (1) C (1) 0.07 0 (0) B (1) B (1) -* 0.11 0 (0) B (1) -* -* 0.14 -* -* -* -* 0.26 C (1) -* -* - 0.44 -* -* -* -* (Supplemental test) Meas.conc.(mg/l) Symtoms 24 hour 48 hour 72 hour 96 hour Control (0) 0 (0) 0 (0) 0 (0) 0 (0) Disper.Con.(0) 0 (0) 0 (0) 0 (0) 0 (0) 0.008 0 (0) 0 (0) 0 (0) 0 (0) 0.014 0 (0) 0 (0) 0 (0) 0 (0) 0: normal A: abnormal respiration B: abnormal swimming ability C: loss of equilibrium or swimming ability D: other symptoms (n): numbers of fish *: No observation was made because all fish were dead at this observation time. - Reference substances (if used) - results: Copper (II) sulfate pentahydrate. 96 h LC50 was 0.29 mg/l. - Any observations, such as precipitation that might cause a difference between measured and nominal values: No precipitates and colour formation by the test chemical. Test condition : Species: Obtained from commercial domestic hatcheries Analytical monitoring: Test solution was measured using by HPLC before and after 24-hour interval Test fish: Acclimated for more than 12 days before testing; any groups showing no mortality for 7 days before test started. Fish with 22.3 mm (19.4 - 24.8 mm) and 23.0 mm (20.3 - 24.4 mm) in length were selected at random for the main test (n=10) and the additional test (n=10), respectively. Average body weight of fish was 0.1866 g and 0.1877 g for the main test (n=10) and the supplemental test (n=10). Test conditions: Details of test: Flow-through (water renewal: 8/day). 76 UNEP PUBLICATIONS
OECD SIDS N-(1,3-DIMETHYLBUTYL)-N´-PHENYL-1,4-PHENYLENEDIAMINE 4. ECOTOXICITY ID: 793-24-8 DATE: 11.05.2005 Dilution water source: Tap water after dechlorinated by passing through activated carbon filter. Dilution water chemistry: Hardness: 41 mg/L as CaCO3; pH: 7.0. Stock and test solution and how they are prepared: Stock solution was prepared daily. Each test solution was prepared by diluting 20 mg/l solution containing 0.02 % HCO-50, prepared daily with diluting water by using a continuous diluting apparatus. Concentrations dosing rate, flowthrough rate, in what medium: Concentrations of the test solutions were 0, 0.15, 0.22, 0.34, 0.51, 0.76, and 1.14 mg/l (main test) and 0.07 and 0.10 mg/l (supplemental test) because fatality was more than 50 % observed at 0.15 mg/l in the main run. Vehicle/solvent and concentrations: HCO-50 was used. Control: dilution water control and dispersant control (11.4 mg/l) were run. Stability of the test chemical solutions: unstable, however, transparent, no precipitate and colour formed during 24-hour monitoring period. Exposure vessel type: 3 l glass beaker. Number of replicates, fish per replicate: 1, 10 individuals/replicate. Water chemistry in test (O2, pH) in the control and one concentration where effects were observed: Dissolved oxygen readings and pH values were taken daily during 96-hour exposure period. Dissolved oxygen concentration: 7.1 - 8.6 mg/l. pH values: 6.7 - 7.1. Test temperature: Water temperature at 23.1 - 23.8 °C during 96-hour exposure period. Method of calculating mean measured concentrations: Geometric mean because the measured concentrations were less than± 20 % of the nominal concentration. Statistical methods: The LC50 (Moving average) was calculated by using TOXDAT Multi Method Program (US EPA) and probit by EcoTox-Statics (ver 1.1,Oita University) Test substance : N-(1,3-<strong>Dimethylbutyl</strong>)-N-phenyl-1,4-phenylendiamine (CAS No. 793 - 24 - 8). Source: Synthesized by the Toray Research Center, Lot No. 99S271C, Purity = 91.0 %. Chemical structure and stability of the chemical during use were confirmed by NMR, IR and HPLC. Kept at room temperature in a dark place until use. Conclusion : LC50 for medaka was determined to be 0.028 mg/L (confident limit: 0.020 - 0.038 mg/L) for 96 hours based on geometric means of measured concentrations. Reliability : (1) valid without restriction Reliable without restriction Experimental design and analytical procedure were well documented Flag : Critical study for SIDS endpoint 08.08.2003 (46) Type : flow through Species : Pimephales promelas (Fish, fresh water) Exposure period : 28 day(s) Unit : mg/l LC50 : = .15 calculated Limit test : Analytical monitoring : Yes Method : other: Methods for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians (US-EPA 1975) Year : 1979 GLP : Yes Test substance : other TS: Santoflex 13 Method : Flow-through system; proportional diluter; exchange rate: 14 times / 24 h; aerated well water at 22 °C (+/- 2 °C), carrier: acetone; monitoring of TS by GC/FID-analysis. Remark : Several aspects of the study have to be clarified - Ammonia: On page 7 of the study it is reported that the well water UNEP PUBLICATIONS 77
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N-(1,3-Dimethylbutyl)-N

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