2013 Promega catalogue

Life
Science
Catalog
2013
Worldwide Contact List
Cell Signaling
Untagged Flexi ® Mammalian Expression
Vectors
Product Size Cat.# Price ($)
pF4A CMV Flexi ® Vector 20 μg C8481 489
pF4K CMV Flexi ® Vector 20 μg C8491 489
pF5A CMV-neo Flexi ® Vector 20 μg C9401 489
pF5K CMV-neo Flexi ® Vector 20 μg C9411 489
pF9A CMV hRluc-neo Flexi ® Vector 20 μg C9361 489
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: These vectors are designed specifically for high-level expression
of proteins in mammalian cells from the CMV promoter with or without a
selectable marker. The pFN9A Vector provides Renilla luciferase, which may be
used as a transfection control. The pFN9A Vector was designed to complement
pGL4 firefly luciferase vectors when exogenous proteins (e.g., a receptor
of transcription factor) must be expressed for reporter assays. All inserts may
be confirmed by cell-free expression with the TNT ® T7 Quick System (Cat.#
L1170).
Note: Flexi ® Vectors contain the lethal barnase gene to reduce background
colonies without inserts during the subcloning procedure. Using the Flexi ® Vector
Cloning System replaces the barnase gene with your insert. These vectors,
as purchased, cannot be cultured in normal laboratory strains of E. coli without
an insert.
Features:
• Versatility: You can choose between a variety of initial applications (e.g.,
bacterial protein, mammalian, or cell-free protein expression) and then
transfer to others as required.
• Time Savings: Efficient transfer allows for direct use of recombinant
clones, minimizing time wasted screening background colonies.
• Enhanced Productivity: Adaptable to high-throughput formats for large
screening projects.
• Easy Access: No licensing fees or complicated transfer restrictions.
Storage Conditions: Store vectors at –20°C.
rrnB
Terminator
cer
ori
T7
Sgf I
Barnase
pF1A T7
Flexi ® Vector
(3455bp)
Amp r
Pme I
T7 Terminator
4815MA
Inducible T7-Driven Flexi ® Vectors for E. coli
Expression
Product Size Cat.# Price ($)
pH6HTN His 6 HaloTag ® T7 Vector 20 μg G7971 363
pH6HTC His 6 HaloTag ® T7 Vector 20 μg G8031 363
pF1A T7 Flexi ® Vector 20 μg C8441 435
pF1K T7 Flexi ® Vector 20 μg C8451 435
pFN18A HaloTag ® T7 Flexi ® Vector 20 μg G2751 405
pFN18K HaloTag ® T7 Flexi ® Vector 20 μg G2681 405
pFN19A HaloTag ® T7 SP6 Flexi ® Vector 20 μg G1891 405
pFN19K HaloTag ® T7 SP6 Flexi ® Vector 20 μg G1841 405
pFC20A HaloTag ® T7 SP6 Flexi ® Vector 20 μg G1681 405
pFC20K HaloTag ® T7 SP6 Flexi ® Vector 20 μg G1691 405
pFN29A His 6 HaloTag ® T7 Flexi ® Vector 20 μg G8261 405
pFN29K His 6 HaloTag ® T7 Flexi ® Vector 20 μg G8331 405
pFC30A His 6 HaloTag ® T7 Flexi ® Vector 20 μg G8321 405
pFC30K His 6 HaloTag ® T7 Flexi ® Vector 20 μg G8381 405
pFN2A (GST) Flexi ® Vector 20 μg C8461 489
pFN2K (GST) Flexi ® Vector 20 μg C8471 489
pFN6A (HQ) Flexi ® Vector 20 μg C8511 489
pFN6K (HQ) Flexi ® Vector 20 μg C8521 489
pFC7A (HQ) Flexi ® Vector 20 μg C8531 489
pFC7K (HQ) Flexi ® Vector 20 μg C8541 489
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: These vectors are designed specifically for expression of proteins
in E. coli from the T7 RNA polymerase promoter with or without the addition
of purification tags. Expression levels can vary from protein to protein, but in
general the N-terminal HaloTag ® fusion protein (e.g., pFN18A/K) can increase
expression by increasing solubility of the expressed protein. Proteins may be
purified using the HaloTag ® fusion with the HaloTag ® Protein Purification System
(Cat.# G6280). Expression may be confirmed through cell-free expression
with the S30 T7 High-Yield Protein Expression System (Cat.# L1110).
The Flexi ® Vector System is a simple, yet powerful, directional cloning method
for protein-coding sequences. It is based on two rare-cutting restriction
enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to
transfer protein-coding regions between a variety of Flexi ® Vectors without the
need to resequence.
Note: Flexi ® Vectors contain the lethal barnase gene to reduce background
colonies without inserts during the subcloning procedure. Using the Flexi ® Vector
Cloning System replaces the barnase gene with your insert. These vectors,
as purchased, cannot be cultured in normal laboratory strains of E. coli without
an insert.
Features:
• Versatility: You can choose between a variety of initial applications (e.g.,
bacterial protein, mammalian, or cell-free protein expression) and then
transfer to others as required.
• Time Savings: Efficient transfer allows for direct use of recombinant
clones, minimizing time wasted screening background colonies.
• Enhanced Productivity: Adaptable to high-throughput formats for large
screening projects.
• Easy Access: No licensing fees or complicated transfer restrictions.
Storage Conditions: Store vectors at –20°C.
Section
Contents
Table of
Contents
114
For complete and up-to-date product information visit: www.promega.com/catalog