2013 Promega catalogue

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Life Science Catalog <strong>2013</strong> Worldwide Contact List Section Contents Cell Signaling MOPS/EDTA Buffer Product Size Cat.# Price ($) MOPS/EDTA Buffer 3 × 10 ml Y5101 66 For Research Use Only. Not for Use in Diagnostic Procedures. Description: MOPS/EDTA Buffer is provided at pH 7.4 for the resuspension and dilution of primers and templates used in the Plexor ® qPCR and qRT-PCR Systems. It is critical that this MOPS/EDTA Buffer be used with the Iso-dCcontaining primers used in the Plexor ® Systems, as these primers are sensitive to pH below 7.0. Storage Conditions: Store at any temperature. StemElite Gene Expression System Product Size Cat.# Price ($) StemElite Gene 100 qPCR reactions B1001 258 Expression System StemElite Gene 100 qPCR reactions + 50 RT reactions B1002 423 Expression System Plus For Research Use Only. Not for Use in Diagnostic Procedures. Description: The StemElite Gene Expression System is a novel real-time quantitative PCR (qPCR) system for the detection and relative quantification of RNA expression levels associated with the differentiation state or ‘potency’ of cells. The StemElite Gene Expression System is optimized to quantitatively amplify a two-color duplex, enabling the user to amplify a transcript of interest as well as a reference gene in a single reaction. Features: • Achieve sensitive real-time detection and relative quantification of RNA expression levels for monitoring stem cell differentiation in a multiplex amplification. • Quantitatively amplify a two-color duplex. • Amplify in a single tube the transcript of interest and reference transcript (GAPDH or Actb). • Reduce the number of reactions required to measure multiple transcripts. • Improve experimental data quality by measuring the transcripts in the same well. Storage Conditions: Store at –20°C. Protocol StemElite Gene Expression System Technical Manual Part# TM323 StemElite Human Pluripotent Transcripts Product Size Cat.# Price ($) StemElite NANOG/GAPDH Primer Pair (20X) 100 μl B1011 134 StemElite SOX2/GAPDH Primer Pair (20X) 100 μl B1021 134 StemElite POU5F1/GAPDH Primer Pair (20X) 100 μl B1031 134 StemElite LIN28/GAPDH Primer Pair (20X) 100 μl B1041 134 StemElite KLF4/GAPDH Primer Pair (20X) 100 μl B1051 134 StemElite MYC/GAPDH Primer Pair (20X) 100 μl B1061 134 For Research Use Only. Not for Use in Diagnostic Procedures. Description: NANOG, SOX2, POU5F1, LIN28, KLF4 and MYC are functionally associated with maintenance of the undifferentiated human embryonic stem cell. Features: • Achieve sensitive real-time detection and relative quantification of RNA expression levels for monitoring stem cell differentiation in a multiplex amplification. • Quantitatively amplify a two-color duplex. • Amplify in a single tube the transcript of interest and reference transcript (GAPDH). • Reduce the number of reactions required to measure multiple transcripts. • Improve experimental data quality by measuring the transcripts in the same well. Storage Conditions: Store at –20°C. StemElite Human Heart-Associated Transcripts Product Size Cat.# Price ($) StemElite NPPA/GAPDH Primer Pair (20X) 100 μl B1071 134 StemElite MYL7/GAPDH Primer Pair (20X) 100 μl B1081 134 StemElite MYL2/GAPDH Primer Pair (20X) 100 μl B1091 134 StemElite MYH6/GAPDH Primer Pair (20X) 100 μl B1101 134 StemElite MYH7/GAPDH Primer Pair (20X) 100 μl B1111 134 StemElite NKX2-5/GAPDH Primer Pair (20X) 100 μl B1121 134 StemElite TNNT2/GAPDH Primer Pair (20X) 100 μl B1131 134 StemElite TNNI3/GAPDH Primer Pair (20X) 100 μl B1141 134 StemElite MEF2C/GAPDH Primer Pair (20X) 100 μl B1151 134 StemElite PLN/GAPDH Primer Pair (20X) 100 μl B1161 134 StemElite GATA4/GAPDH Primer Pair (20X) 100 μl B1171 134 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Pluripotential stem cells can give rise to differentiated cells and tissues for all three embryonic germ layers. NPPA, MYL7, MYL2, MYH6, MYH7, NKX2-5, TNNT2, TNNI3, MEF2C, PLN and GATA4 are mesodermal markers associated with differentiation of cardiac muscle. Features: • Achieve sensitive real-time detection and relative quantification of RNA expression levels for monitoring stem cell differentiation in a multiplex amplification. • Quantitatively amplify a two-color duplex. • Amplify in a single tube the transcript of interest and reference transcript (GAPDH). • Reduce the number of reactions required to measure multiple transcripts. • Improve experimental data quality by measuring the transcripts in the same well. Storage Conditions: Store at –20°C. Table of Contents 248 For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling StemElite Human Pancreatic-Associated Transcripts Product Size Cat.# Price ($) StemElite HNF4A/GAPDH Primer Pair (20X) 100 μl B1301 134 StemElite HNF1B/GAPDH Primer Pair (20X) 100 μl B1311 134 StemElite PDX1/GAPDH Primer Pair (20X) 100 μl B1321 134 StemElite INS/GAPDH Primer Pair (20X) 100 μl B1331 134 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Pluripotential stem cells can give rise to differentiated cells and tissues for all three embryonic germ layers. HNF4A, HNF1B, PDX1 and INS are mesodermal markers associated with differentiation of pancreatic cells. Features: • Achieve sensitive real-time detection and relative quantification of RNA expression levels for monitoring stem cell differentiation in a multiplex amplification. • Quantitatively amplify a two-color duplex. • Amplify in a single tube the transcript of interest and reference transcript (GAPDH). • Reduce the number of reactions required to measure multiple transcripts. • Improve experimental data quality by measuring the transcripts in the same well. Storage Conditions: Store at –20°C. StemElite Differentiation-Associated Transcripts Product Size Cat.# Price ($) StemElite FOXA2/GAPDH Primer Pair (20X) 100 μl B1341 134 StemElite SOX17/GAPDH Primer Pair (20X) 100 μl B1351 134 StemElite GATA6/GAPDH Primer Pair (20X) 100 μl B1361 134 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Pluripotential stem cells can give rise to differentiated cells and tissues for all three embryonic germ layers. FOXA2, SOX17 and GATA6 are nonspecific differentiation markers. Features: • Achieve sensitive real-time detection and relative quantification of RNA expression levels for monitoring stem cell differentiation in a multiplex amplification. • Quantitatively amplify a two-color duplex. • Amplify in a single tube the transcript of interest and reference transcript (GAPDH). • Reduce the number of reactions required to measure multiple transcripts. • Improve experimental data quality by measuring the transcripts in the same well. Storage Conditions: Store at –20°C. StemElite Mouse Pluripotent Transcripts Product Size Cat.# Price ($) StemElite Mus-Nanog/Actb Primer Pair (20X) 100 μl B1371 134 StemElite Mus-Sox2/Actb Primer Pair (20X) 100 μl B1381 134 StemElite Mus-Pou5f1/Actb Primer Pair (20X) 100 μl B1391 134 StemElite Mus-Lin28/Actb Primer Pair (20X) 100 μl B1401 134 StemElite Mus-Klf4/Actb Primer Pair (20X) 100 μl B1411 134 StemElite Mus-Myc/Actb Primer Pair (20X) 100 μl B1421 134 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Mus-Nanog, Mus-Sox2, Mus-Pou5f1, Mus-Lin28, Mus-Klf4 and Mus-Myc are functionally associated with maintenance of the undifferentiated mouse embryonic stem cell. Features: • Achieve sensitive real-time detection and relative quantification of RNA expression levels for monitoring stem cell differentiation in a multiplex amplification. • Quantitatively amplify a two-color duplex. • Amplify in a single tube the transcript of interest and reference transcript (Actb). • Reduce the number of reactions required to measure multiple transcripts. • Improve experimental data quality by measuring the transcripts in the same well. Storage Conditions: Store at –20°C. RT-PCR GoScript Reverse Transcription System Product Size Cat.# Price ($) GoScript Reverse Transcription System 50 reactions A5000 450 100 reactions A5001 800 Available Separately Size Cat.# Price ($) GoScript Reverse Transcriptase 100 reactions A5003 388 500 reactions A5004 1450 For Laboratory Use. Description: The GoScript Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of firststrand cDNA optimized for quantitative PCR amplification. GoScript Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors. GoScript Reverse Transcriptase is qualified for use in qPCR, including GoTaq ® qPCR and Plexor ® RT-qPCR systems. Features: • Ultra-Active: Save money on every reaction. • Sensitive: Detect rare transcripts. • Processive: Transcribe long messages. • Resilient: Synthesize cDNA in the presence of strong inhibitors. Storage Conditions: Store at –20°C. 14 PCR Protocol GoScript Reverse Transcription System Technical Manual Part# TM316 Section Contents For complete and up-to-date product information visit: www.promega.com/catalog 249 Table of Contents
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Life Science CATALOG 2013 Table of
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Cell Signaling Table of Contents Pr
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Cell Signaling 1 Biobanking 1 DNA E
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Cell Signaling DNA Extraction for B
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Cell Signaling Wizard ® Genomic DN
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Cell Signaling QuantiFluor RNA Syst
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Cell Signaling Sample ID and Mixed
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Cell Signaling 2 Biochemicals and L
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling SDS Solution, Molecu
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Magnetic Stands and
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Cell Signaling 3 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 8 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling CellTiter-Glo ® One
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Cell Signaling Fluorescent Cell Via
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Cell Signaling CellTiter-Blue ® Ce
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Cell Signaling ApoTox-Glo Triplex A
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Cell Signaling CytoTox-Fluor Cytoto
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 4 Cell Signaling 4 G
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Kinase Assay
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Cell Signaling Kinase Enzyme System
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Cell Signaling ProFluor ® PKA Assa
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Cell Signaling PepTag ® Non-Radioa
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Cell Signaling Product Size Cat.# P
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Cell Signaling kDa 98 - 64 - 50 - 3
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Cell Signaling ProFluor ® Ser/Thr
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Cell Signaling 5 Cloning and DNA Ma
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling RNA Markers Product
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Cell Signaling AatII 37 Product Siz
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Cell Signaling BstEII 60 Product Si
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Cell Signaling HhaI 37 Product Size
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Cell Signaling NdeI 37 Product Size
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Cell Signaling SnaBI 37 Product Siz
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Cell Signaling Alkaline Phosphatase
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Cell Signaling T4 DNA Polymerase Pr
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Cell Signaling Kinases and DNA Labe
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Cell Signaling Additional Enzymes S
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Cell Signaling Subcloning Tools and
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Cell Signaling pALTER ® -MAX Vecto
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Cell Signaling pGEM ® -7Zf(+/-) Ve
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Cell Signaling pSP72 Vector Product
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Cell Signaling 6 DNA and RNA Purifi
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep Blood gDNA
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Cell Signaling Wizard ® SV 96 Geno
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Cell Signaling Fixed-Tissue Genomic
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Cell Signaling Maxwell ® 16 System
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Cell Signaling PureYield Plasmid Mi
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Cell Signaling Wizard ® Plus Minip
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Cell Signaling Wizard ® SV 96 and
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Cell Signaling RNA Purification Rel
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA and RNA Quantita
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Cell Signaling Magnetic Stands and
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Cell Signaling Vacuum Manifolds and
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Cell Signaling 7 Drug Discovery 7 D
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling GloResponse Lucifera
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Cell Signaling DPPIV-Glo Protease A
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Cell Signaling Calpain-Glo Protease
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Cell Signaling 8 Epigenetics 8 DNA
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Cell Signaling DNA Purification Tec
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Cell Signaling Luciferase-Based Met
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Cell Signaling GoTaq ® Long PCR Ma
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Cell Signaling DUB-Glo Protease Ass
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Cell Signaling HaloTag ® Protein P
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Cell Signaling 9 Genetic Identity 9
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Cell Signaling DNA IQ System Produc
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Cell Signaling DNA IQ Casework Pro
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Cell Signaling STR Analysis for For
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Cell Signaling PowerPlex ® Y23 Sys
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® S5 Syst
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Cell Signaling GenePrint ® Fluores
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Cell Signaling 10 Imaging and Immun
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Cell Signaling HaloTag ® Ligand Bu
Page 201 and 202: Cell Signaling NGF E max ® ImmunoA
Page 203 and 204: Cell Signaling Anti-ACTIVE ® CaM K
Page 205 and 206: Cell Signaling A. Anti-ACTIVE ® MA
Page 207 and 208: Cell Signaling Anti-ACTIVE ® Caspa
Page 209 and 210: Cell Signaling Anti-NGF mAb Product
Page 211 and 212: Cell Signaling Anti-TGFβ 1 pAb Pro
Page 213 and 214: Cell Signaling Horseradish Peroxida
Page 215 and 216: Cell Signaling ViviRen In Vivo Reni
Page 217 and 218: Cell Signaling 11 Industrial and En
Page 219 and 220: Cell Signaling QuantiLum ® Recombi
Page 221 and 222: 12 Instruments 12 Luminometers 218
Page 223 and 224: GloMax ® 20/20 Luminometer Product
Page 225 and 226: Fluorescence Module: Application-op
Page 227 and 228: GloMax ® -Multi Jr Single-Tube Mul
Page 229 and 230: Maxwell ® CSC System for IVD Use M
Page 231 and 232: Maxwell ® Research Systems Maxwell
Page 233 and 234: Maxwell ® 16 Flexi Method Firmware
Page 235 and 236: Cell Signaling 13 Molecular Diagnos
Page 237 and 238: Cell Signaling Maxwell ® CSC Servi
Page 239 and 240: Cell Signaling Maxwell ® 16 System
Page 241 and 242: Cell Signaling Maxwell ® 16 Flexi
Page 243 and 244: Cell Signaling Deoxynucleotide Trip
Page 245 and 246: Cell Signaling 14 PCR 14 Hot-Start
Page 247 and 248: Cell Signaling Routine PCR GoTaq ®
Page 249 and 250: Cell Signaling Pfu DNA Polymerase d
Page 251: Cell Signaling GoTaq ® Real-Time q
Page 255 and 256: Cell Signaling Reverse Transcriptio
Page 257 and 258: Cell Signaling AMV Reverse Transcri
Page 259 and 260: Cell Signaling PCR Cloning pGEM ®
Page 261 and 262: Cell Signaling 15 Protein Expressio
Page 263 and 264: Cell Signaling TnT ® Quick Coupled
Page 265 and 266: Cell Signaling TnT ® T7 Quick for
Page 267 and 268: Cell Signaling Canine Pancreatic Mi
Page 269 and 270: Cell Signaling E. coli S30 Extract
Page 271 and 272: Cell Signaling Mass Spectrometry An
Page 273 and 274: Cell Signaling Endoproteinase Lys-C
Page 275 and 276: Cell Signaling Protein Labeling and
Page 277 and 278: Cell Signaling HaloTag ® Fusion (C
Page 279 and 280: Cell Signaling Inducible T7-Driven
Page 281 and 282: Cell Signaling ECL Western Blotting
Page 283 and 284: Cell Signaling 16 Protein Purificat
Page 285 and 286: Cell Signaling HaloTag ® Protein P
Page 287 and 288: HT Cell Signaling HaloTag ® Mammal
Page 289 and 290: Cell Signaling HaloLink Protein Arr
Page 291 and 292: Cell Signaling FastBreak Cell Lysis
Page 293 and 294: Cell Signaling MagneHis Protein Pur
Page 295 and 296: Cell Signaling Streptavidin Product
Page 297 and 298: Cell Signaling Protein Purification
Page 299 and 300: Cell Signaling 17 Reporter Assays a
Page 301 and 302: Cell Signaling NanoLuc Luciferase
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Cell Signaling Chroma-Glo Luciferas
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Cell Signaling ONE-Glo + Tox Lucife
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Cell Signaling Luciferase Assay Sys
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Cell Signaling ViviRen Live Cell Su
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Cell Signaling Reporter Vectors and
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Cell Signaling Promoterless Renilla
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Cell Signaling pmirGLO Dual-Lucifer
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Cell Signaling pGL2 Luciferase Repo
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Cell Signaling Reporter Vector Sequ
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Cell Signaling pCAT ® 3 Vectors Pr
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Cell Signaling ViviRen In Vivo Reni
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Cell Signaling 18 RNA Analysis 18 I
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 19 Stem Cell Researc
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Cell Signaling Cell ID System Fluor
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Cell Signaling StemElite Human Panc
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Cell Signaling 20 STR Analysis 20 C
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Cell Signaling PowerPlex ® 16 HS S
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Cell Signaling StemElite ID System
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Cell Signaling 21 Vectors 21 Bacter
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Cell Signaling Mammalian Expression
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Cell Signaling pCI-neo Mammalian Ex
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Cell Signaling pSV-β-Galactosidase
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22 Index 22 Index: A-Z 354 Index by
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Index: A-Z Click on name to go to p
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Index by Catalog Number Click on pa
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1S11 TH01 D3S1358 FGA TPOX D8S1179
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