2013 Promega catalogue

Life
Science
Catalog
2013
Worldwide Contact List
Cell Signaling
GoTaq ® PCR Core Systems
Product Size Cat.# Price ($)
GoTaq ® PCR Core System I 200 reactions M7660 318
GoTaq ® PCR Core System II 200 reactions M7665 345
M7660, M7665 For Laboratory Use.
Description: The GoTaq ® PCR Core Systems I and II are designed for the
exponential amplification of specific regions of DNA using PCR. Both systems
include GoTaq ® DNA polymerase and PCR Nucleotide Mix, along with highperformance
buffers and magnesium chloride. The GoTaq ® PCR Core System II
also includes a Positive Control Plasmid DNA and Positive Control Primers,
providing increased confidence in PCR control reactions. Each system’s components
are performance-tested in PCR and are sufficient for 200 reactions. The
GoTaq ® PCR Core Systems are provided with a Technical Bulletin that provides
simple protocols and a complete troubleshooting guide.
Features:
• Convenience: PCR-tested components are provided in optimized volumes
for 200 reactions.
• Flexibility: Optimization tools are provided for reaction flexibility.
• Positive Controls: The GoTaq ® PCR Core System II provides Positive Control
Plasmid DNA and Control Primers to help troubleshoot PCR parameters.
• Performance Guarantee: Promega PCR systems, enzymes and reagents
are proven in PCR to ensure reliable, high-performance results. If you are
not completely satisfied with any Promega PCR product, we will send a
replacement or refund your account.
Storage Conditions: Store all components at –20°C.
Protocol
GoTaq ® PCR Core Systems Technical Bulletin
PCR Master Mix
Part#
TB254
Product Size Conc. Cat.# Price ($)
PCR Master Mix 10 reactions 2 X M7501 21
100 reactions 2 X M7502 108
1,000 reactions 2 X M7505 783
For Laboratory Use.
Description: PCR Master Mix is a premixed, ready-to-use solution containing
Taq DNA polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations
for efficient amplification of DNA templates by PCR. The PCR Master Mix
is optimized for use in routine PCR for amplifying DNA templates in the range
of 0.2–2kb.
Features:
• Fast: Set up reactions in less than a minute.
• Sensitive: Amplify as little as 2 copies of target template.
• Convenient: One tube, one pipetting step.
• Complete: Reagents, including Taq DNA polymerase, MgCl 2 , dNTPs and
buffers, in one tube.
• Scalable: Set up 10μl, 25μl or 50μl reactions.
• Stable: Store at 4°C for up to 3 months.
• Performance Guaranteed: Promega PCR systems, enzymes and
reagents are proven in PCR to ensure reliable, high-performance results.
If you are not completely satisfied with any Promega PCR product, we will
send a replacement or refund your account.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C. PCR Master Mix can be stored at 4°C
for up to 3 months.
M
Template Copies per Reaction
0
2
10
100
1,000
Detection of low-copy-number templates using PCR Master Mix. Use
of PCR Master Mix to detect low number of copies of the α-1-antitrypsin
gene. PCR was performed on Human Genomic DNA (Cat.# G3041) using
primers targeting a 360bp fragment of the α-1-antitrypsin gene (single
copy per genome). Lane M, 100bp DNA Ladder (Cat.# G2101).
Tfl DNA Polymerase
Product Size Conc. Cat.# Price ($)
Tfl DNA Polymerase 100 u 5 u/µl M1941 105
1,000 u 5 u/µl M1945 666
For Laboratory Use.
Description: Tfl DNA Polymerase is a thermostable enzyme of approximately
94kDa isolated from Thermus flavus. The enzyme replicates DNA at 74°C
and exhibits a half-life of 40 minutes at 95°C. Tfl DNA Polymerase catalyzes
the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in
the presence of magnesium and the polymerization of nucleotides into DNA
using an RNA template in the 5´→3´ direction in the presence of manganese.
The enzyme also possesses a 5´→3´ exonuclease activity. Tfl DNA Polymerase
is recommended for use in PCR and primer extension reactions at elevated
temperatures.
Tfl DNA Polymerase 10X Reaction Buffer: 200mM Tris-acetate (pH 8.9 at
25°C), 100mM ammonium sulfate.
Magnesium Sulfate: 25mM MgSO 4 Solution included.
Features:
• Flexibility: Provided with a 10X Reaction Buffer that does not contain
magnesium. Sufficient 25mM MgSO 4 is provided separately to allow
optimization of enzyme performance under different conditions.
• Performance Guarantee: Promega PCR systems, enzymes and reagents
are proven in PCR to ensure reliable, high-performance results. If you are
not completely satisfied with any Promega PCR product, we will send a
replacement or refund your account.
Storage Conditions: Store at –20°C.
M
3063TA09_0A
Section
Contents
Protocol
PCR Master Mix Protocol
Part#
9PIM750
Table of
Contents
244
For complete and up-to-date product information visit: www.promega.com/catalog