2013 Promega catalogue

Cell Signaling
NanoLuc Luciferase Reporter
Systems
Nano-Glo Luciferase Assay System
Product Size Cat.# Price ($)
Nano-Glo Luciferase Assay 10 ml N1110 232
For Research Use Only. Not for Use in Diagnostic Procedures.
100 ml N1120 1323
10 × 10 ml N1130 1588
10 × 100 ml N1150 Pls. Enq.
Description: Nano-Glo Luciferase Assay Reagent is a dedicated product
for the detection of NanoLuc luciferase. For details on NanoLuc luciferase
technology, see page 307.
The Nano-Glo Luciferase Assay System provides a simple, single-addition
reagent that generates a glow-type signal in the presence of NanoLuc
luciferase with a half-life of approximately 120 minutes in commonly used
tissue culture media. The reagent is prepared by mixing Nano-Glo luciferase
Assay Substrate and Nano-Glo Luciferase Assay Buffer. The reagent contains
an integral lysis buffer allowing use directly on cells expressing NanoLuc
luciferase or the culture media when luciferase is secreted.
Features:
• Work on Advanced Reporter Systems: Bright NanoLuc reporter
allows use in challenging applications where sensitivity is limited.
• Simplify Your Assay Optimization: Add-and-read simplicity allows
scaling from bench to HTS.
• Improve Assay Precision: No need for separate lysis and reagent
injection steps.
• Get a Brighter, Longer-Lasting Signal: Extended bright light output is
optimized for batch and continuous-process handling.
Protocol
Nano-Glo Luciferase Assay System Technical Manual
Part#
TM369
Dual-Luciferase Reporter Systems
Dual-Glo ® Luciferase Assay System
Product Size Cat.# Price ($)
Dual-Glo ® Luciferase Assay System 10 ml E2920 479
100 ml E2940 Pls. Enq.
10 × 100 ml E2980 Pls. Enq.
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Dual-Glo ® Luciferase Assay System is a homogeneous
reagent system that enables fast and simple quantitation of a stable
luminescent signal from two reporter genes in a single sample. This convenient
“add-and-read” system generates both firefly and Renilla luciferase
luminescence signals from cells that have not been preconditioned or prelysed.
The Dual-Glo ® Luciferase Assay System provides high Z´-factors for cell-based,
high-throughput screening applications. With the Dual-Glo ® System, internal
controls can be established to minimize sample variability by reducing false
positive and false negative readings caused by nonspecific factors such as
cytotoxicity. In the Dual-Glo ® Luciferase Assay, the activity of the primary
reporter is correlated with the effect of specific stimuli, and the activity of the
co-transfected control reporter provides an internal control to normalize results.
The system is optimized for batch processing both 96- and 384-well plates
and is compatible with a wide variety of mammalian cell culture media.
Features:
• Increased Precision and Accuracy: Normalize primary reporter results
with an internal control, a co-reporter that minimizes effects of cell number
and health, transfection efficiency and nonspecific cellular responses.
• Homogeneous Format: Perform fewer steps. Assay cells directly in
growth medium for both reporters. No centrifugation or lysis step is
required.
• Stable Signal: Obtain flexibility for either batch or continuous processing
of 96- and 384-well plates. Each luminescent signal may be measured for
up to 2 hours after reagent addition.
• Convenience: Screen efficiently with this simple, two-step assay that is
ideal for any luminometer. On-board injectors not required.
• Wide Dynamic Range: Analyze high and low reporter activity without
sample dilution. Linear over at least 6 logs of enzyme concentration for
each reporter.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store Dual-Glo ® Substrates at –20°C. Store Dual-Glo ®
Buffers below 25°C.
Protocol
Dual-Glo ® Luciferase Assay System Technical Manual
Part#
TM058
17
Reporter Assays and Transfection
O
N
N
H
N
O
+ O 2
NanoLuc
O
Luciferase
N NH
N
O
+ CO 2
+ Light
Furimazine
Furimamide
The bioluminescent reaction catalyzed by NanoLuc luciferase.
10559MA
Section
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For complete and up-to-date product information visit: www.promega.com/catalog
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