2013 Promega catalogue

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Life Science Catalog <strong>2013</strong> Worldwide Contact List Cell Signaling psiCHECK-1 and psiCHECK-2 Vectors Product Size Cat.# Price ($) psiCHECK-1 Vector 20 μg C8011 464 psiCHECK-2 Vector 20 μg C8021 464 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The psiCHECK-1 and psiCHECK-2 Vectors are designed to provide a quantitative and rapid approach for initial optimization of RNA interference (RNAi). The vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. In both vectors Renilla luciferase is used as the primary reporter gene, and the gene of interest is cloned into a multiple cloning region located downstream of the Renilla translational stop codon. Initiation of the RNAi process by synthetic siRNAs or in vivo-expressed shRNAs toward a gene of interest results in cleavage and subsequent degradation of the fusion mRNA. Measuring decreases in Renilla activity provides a convenient way of monitoring the RNAi effect. In comparison with other fusion approaches (e.g., GFP or flag-tags), the Renilla luciferase approach offers more convenient and rapid quantitation with higher sensitivity. The psiCHECK-1 Vector is recommended for use in monitoring RNAi effects in live cells. The changes in Renilla luciferase activity are measured with the EnduRen Live Cell Substrate (Cat.# E6481), which allows continuous monitoring of intracellular Renilla luminescence. The psiCHECK-2 Vector contains a second reporter gene, firefly luciferase, and is designed for endpoint lytic assays. Introduction of firefly luciferase in the psiCHECK-2 Vector allows normalization of Renilla luciferase expression, achieving robust and reproducible results. Features: • Save Money: Quantitation is performed with a common luminometer; no need to purchase expensive equipment. • Choose Your Format: Protocols allow for measurements in live cells or crude cell lysates. • Save Time: No requirement for labor-intensive, time-consuming assays or waiting for phenotypic changes. • Convenient: No requirement for transfection normalization when using the psiCHECK-2 Vector. Storage Conditions: Store at –20°C. Protocol siCHECK Vectors Part# TB329 BamHI 1738 1674 NotI 1663 PmeI 1654 EcoRI 1651 SmaI 1643 XhoI 1640 SgfI HSV-TK promoter 1674 NotI 1663 PmeI 1643 XhoI 1640 SgfI Synthetic poly(A) Amp r hRluc BamHI 4451 SV40 Late poly(A) hRluc hluc+ Synthetic poly(A) psiCHECK-1 Vector (3560bp) hRluc T7 Promoter Amp r psiCHECK-2 Vector (6273bp) translation stop T7 Promoter ori NheI 684 BglII 1 KpnI 58 SV40 early enhancer/ promoter HindIII 420 PstI 462 ori NheI 684 gene of interest BglII 1 KpnI 58 SV40 early enhancer/ promoter 4343MA10_3A 4345MA10_3A 5´ 3´ mRNA + shRNA (21–23bp) 5´ 3´ 5´ 3´ translation cleavage of mRNA degradation hRluc light hRluc no light 4339MA10_3A Section Contents Mechanism of action of the psiCHECK Vectors. Table of Contents 328 For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling 19 Stem Cell Research 19 Cell Line Authentication 330 Stemness Assessment 332 Stem Cell Research Section Contents For complete and up-to-date product information visit: www.promega.com/catalog 329 Table of Contents
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Life Science CATALOG 2013 Table of
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Cell Signaling Table of Contents Pr
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Cell Signaling 1 Biobanking 1 DNA E
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Cell Signaling DNA Extraction for B
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Cell Signaling Wizard ® Genomic DN
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Cell Signaling QuantiFluor RNA Syst
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Cell Signaling Sample ID and Mixed
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Cell Signaling 2 Biochemicals and L
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling SDS Solution, Molecu
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Magnetic Stands and
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Cell Signaling 3 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 8 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling CellTiter-Glo ® One
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Cell Signaling Fluorescent Cell Via
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Cell Signaling CellTiter-Blue ® Ce
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Cell Signaling ApoTox-Glo Triplex A
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Cell Signaling CytoTox-Fluor Cytoto
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 4 Cell Signaling 4 G
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Kinase Assay
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Cell Signaling Kinase Enzyme System
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Cell Signaling ProFluor ® PKA Assa
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Cell Signaling PepTag ® Non-Radioa
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Cell Signaling Product Size Cat.# P
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Cell Signaling kDa 98 - 64 - 50 - 3
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Cell Signaling ProFluor ® Ser/Thr
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Cell Signaling 5 Cloning and DNA Ma
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling RNA Markers Product
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Cell Signaling AatII 37 Product Siz
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Cell Signaling BstEII 60 Product Si
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Cell Signaling HhaI 37 Product Size
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Cell Signaling NdeI 37 Product Size
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Cell Signaling SnaBI 37 Product Siz
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Cell Signaling Alkaline Phosphatase
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Cell Signaling T4 DNA Polymerase Pr
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Cell Signaling Kinases and DNA Labe
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Cell Signaling Additional Enzymes S
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Cell Signaling Subcloning Tools and
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Cell Signaling pALTER ® -MAX Vecto
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Cell Signaling pGEM ® -7Zf(+/-) Ve
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Cell Signaling pSP72 Vector Product
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Cell Signaling 6 DNA and RNA Purifi
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep Blood gDNA
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Cell Signaling Wizard ® SV 96 Geno
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Cell Signaling Fixed-Tissue Genomic
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Cell Signaling Maxwell ® 16 System
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Cell Signaling PureYield Plasmid Mi
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Cell Signaling Wizard ® Plus Minip
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Cell Signaling Wizard ® SV 96 and
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Cell Signaling RNA Purification Rel
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Cell Signaling PureYield RNA Midipr
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA and RNA Quantita
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Cell Signaling Magnetic Stands and
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Cell Signaling Vacuum Manifolds and
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Cell Signaling 7 Drug Discovery 7 D
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling GloResponse Lucifera
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Cell Signaling DPPIV-Glo Protease A
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Cell Signaling Calpain-Glo Protease
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Cell Signaling 8 Epigenetics 8 DNA
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Cell Signaling DNA Purification Tec
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Cell Signaling Luciferase-Based Met
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Cell Signaling GoTaq ® Long PCR Ma
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Cell Signaling DUB-Glo Protease Ass
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Cell Signaling HaloTag ® Protein P
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Cell Signaling 9 Genetic Identity 9
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Cell Signaling DNA IQ System Produc
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Cell Signaling DNA IQ Casework Pro
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Cell Signaling STR Analysis for For
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Cell Signaling PowerPlex ® Y23 Sys
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® S5 Syst
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Cell Signaling GenePrint ® Fluores
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Cell Signaling 10 Imaging and Immun
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling NGF E max ® ImmunoA
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Cell Signaling Anti-ACTIVE ® CaM K
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Cell Signaling A. Anti-ACTIVE ® MA
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling Anti-NGF mAb Product
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Cell Signaling Anti-TGFβ 1 pAb Pro
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Cell Signaling Horseradish Peroxida
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Cell Signaling ViviRen In Vivo Reni
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Cell Signaling 11 Industrial and En
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Cell Signaling QuantiLum ® Recombi
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12 Instruments 12 Luminometers 218
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® CSC System for IVD Use M
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Maxwell ® Research Systems Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Cell Signaling 13 Molecular Diagnos
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Cell Signaling Maxwell ® CSC Servi
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Cell Signaling Maxwell ® 16 Flexi
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Cell Signaling Deoxynucleotide Trip
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Cell Signaling 14 PCR 14 Hot-Start
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Cell Signaling Routine PCR GoTaq ®
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Cell Signaling Pfu DNA Polymerase d
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Cell Signaling GoTaq ® Real-Time q
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Cell Signaling StemElite Human Panc
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Cell Signaling Reverse Transcriptio
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Cell Signaling AMV Reverse Transcri
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Cell Signaling PCR Cloning pGEM ®
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Cell Signaling 15 Protein Expressio
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Cell Signaling TnT ® Quick Coupled
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Cell Signaling TnT ® T7 Quick for
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Cell Signaling Canine Pancreatic Mi
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Cell Signaling E. coli S30 Extract
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Cell Signaling Mass Spectrometry An
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Cell Signaling Endoproteinase Lys-C
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Cell Signaling Protein Labeling and
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Cell Signaling HaloTag ® Fusion (C
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Cell Signaling Inducible T7-Driven
Page 281 and 282: Cell Signaling ECL Western Blotting
Page 283 and 284: Cell Signaling 16 Protein Purificat
Page 285 and 286: Cell Signaling HaloTag ® Protein P
Page 287 and 288: HT Cell Signaling HaloTag ® Mammal
Page 289 and 290: Cell Signaling HaloLink Protein Arr
Page 291 and 292: Cell Signaling FastBreak Cell Lysis
Page 293 and 294: Cell Signaling MagneHis Protein Pur
Page 295 and 296: Cell Signaling Streptavidin Product
Page 297 and 298: Cell Signaling Protein Purification
Page 299 and 300: Cell Signaling 17 Reporter Assays a
Page 301 and 302: Cell Signaling NanoLuc Luciferase
Page 303 and 304: Cell Signaling Chroma-Glo Luciferas
Page 305 and 306: Cell Signaling ONE-Glo + Tox Lucife
Page 307 and 308: Cell Signaling Luciferase Assay Sys
Page 309 and 310: Cell Signaling ViviRen Live Cell Su
Page 311 and 312: Cell Signaling Reporter Vectors and
Page 313 and 314: Cell Signaling Promoterless Renilla
Page 315 and 316: Cell Signaling pmirGLO Dual-Lucifer
Page 317 and 318: Cell Signaling pGL2 Luciferase Repo
Page 319 and 320: Cell Signaling Reporter Vector Sequ
Page 321 and 322: Cell Signaling pCAT ® 3 Vectors Pr
Page 323 and 324: Cell Signaling ViviRen In Vivo Reni
Page 325 and 326: Cell Signaling 18 RNA Analysis 18 I
Page 327 and 328: Cell Signaling Riboprobe ® System
Page 329 and 330: Cell Signaling HeLaScribe ® Nuclea
Page 331: Cell Signaling RNA Interference Gen
Page 335 and 336: Cell Signaling Cell ID System Fluor
Page 337 and 338: Cell Signaling StemElite Human Panc
Page 339 and 340: Cell Signaling 20 STR Analysis 20 C
Page 341 and 342: Cell Signaling PowerPlex ® 16 HS S
Page 343 and 344: Cell Signaling StemElite ID System
Page 345 and 346: Cell Signaling 21 Vectors 21 Bacter
Page 347 and 348: Cell Signaling Mammalian Expression
Page 349 and 350: Cell Signaling pCI-neo Mammalian Ex
Page 351 and 352: Cell Signaling Product Size Cat.# P
Page 353 and 354: Cell Signaling pSV-β-Galactosidase
Page 355 and 356: Cell Signaling Product Size Cat.# P
Page 357 and 358: 22 Index 22 Index: A-Z 354 Index by
Page 359 and 360: Index: A-Z Click on name to go to p
Page 365 and 366: Index by Catalog Number Click on pa
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Index by Catalog Number Click on pa
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Index by Catalog Number Click on pa
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1S11 TH01 D3S1358 FGA TPOX D8S1179
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2013 Promega catalogue

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