2013 Promega catalogue

Cell Signaling
SDS Solution, Molecular Biology Grade
(10% w/v)
Product Size Cat.# Price ($)
SDS Solution, Molecular Biology Grade (10% w/v) 100 ml V6551 63
500 ml V6553 215
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: SDS Solution (10% w/v) is sodium dodecyl sulfate in distilled,
deionized water. SDS is a detergent that is known to denature proteins. It is
used in polyacrylamide gel electrophoresis for the determination of protein
molecular weight. It is also used in nucleic acid extraction procedures for the
disruption of cell walls and dissociation of nucleic acid:protein complexes.
Properties:
• A 260 : ≤0.3.
• A 280 : ≤0.2.
Features:
• Quality Tested: Each lot of SDS Solution is tested and certified to be free
of DNase and RNase activity.
Storage Conditions: Store at 22–25°C.
Sephacryl ® S-400
Product Size Cat.# Price ($)
Sephacryl ® S-400 10 ml V3181 76
For Laboratory Use.
Description: Sephacryl ® S-400 is a chromatography matrix used for rapid
gel filtration. This matrix is useful in experiments involving the incorporation
of synthetic linkers and adaptors. After linker ligation and digestion with the
appropriate enzyme, unincorporated linkers and linker fragments may be
rapidly removed from the DNA sample using spin columns containing
Sephacryl ® S-400. Such columns may be used to separate small DNA
fragments (≤271bp) from longer DNA molecules.
Composition: Suspension in 10mM Tris-HCl (pH 8.0), 100mM NaCl and
1mM EDTA.
Features:
• Quality Tested: Each lot is tested and certified to be free of DNase and
RNase activity.
Storage Conditions: Store at 4°C.
Sodium Chloride, Molecular Biology Grade
Product Size Cat.# Price ($)
Sodium Chloride, Molecular Biology Grade 500 g H5271 57
1 kg H5273 102
For Research Use Only. Not for Use in Diagnostic Procedures.
Sodium Dodecyl Sulfate,
Molecular Biology Grade (SDS)
Product Size Cat.# Price ($)
Sodium Dodecyl Sulfate, Molecular Biology Grade 100 g H5113 68
(SDS)
500 g H5114 241
1 kg H5115 453
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Sodium Dodecyl Sulfate, Molecular Biology Grade (SDS), is a
detergent that is known to denature proteins. It is used in denaturing polyacrylamide
gel electrophoresis for the determination of protein molecular
weight. It is also used in nucleic acid extraction procedures for the disruption
of cell walls and dissociation of nucleic acid:protein complexes.
Formula Weight: 288.38.
Properties:
• Purity: ≥99.5%.
• pH at 25°C (3% w/v): 6.0–7.5.
• A 230 at 3%: ≤0.40.
• A 260 at 3%: ≤0.30.
• A 280 at 3%: ≤0.05.
• A 405 at 3%: ≤0.01.
Features:
• Quality Tested: Each lot is tested and certified to be free of DNase and
RNase activity.
Storage Conditions: Store at 22–25°C.
SSC Buffer, 20X, Molecular Grade
Product Size Cat.# Price ($)
SSC Buffer, 20X, Molecular Grade 1,000 ml V4261 80
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: SSC Buffer, 20X, Molecular Grade (pH 7.0), is commonly used
in nucleic acid hybridization techniques at concentrations from 0.1X to 20X,
depending on the application.
Form: Clear, colorless liquid.
Composition: 3M NaCl, 0.3M sodium citrate (for 20X concentration).
Properties:
• pH at 25°C (20X): 6.9–7.1.
• Lead: ≤10ppm.
• Conductivity at 25°C (2X): 24.4–32.4mmhos.
Features:
• Quality Tested: Each lot of SSC Buffer is tested and certified to be free of
DNase, RNase and protease activity.
Storage Conditions: Store at 22–25°C.
2
Biochemicals and Labware
Description: Sodium Chloride, Molecular Biology Grade, is commonly used in
many molecular biology and forensic applications.
Formula Weight: 58.45.
Properties:
• Purity: ≥99.5%.
• Iron: ≤2ppm.
• Lead: ≤5ppm.
• pH at 25°C of 1M: 5.0–8.0.
• A 260 at 1M: ≤0.02.
• A 280 at 1M: ≤0.01.
• Conductivity at 25°C (0.05M): 5,000–7,000μSm.
Features:
• Quality Tested: Each lot of Sodium Chloride is tested and certified to be
free of DNase, RNase and protease activity.
Storage Conditions: Store at 22–25°C.
Streptavidin
For complete and up-to-date product information visit: www.promega.com/catalog
Product Size Cat.# Price ($)
Streptavidin 1 mg Z7041 88
For Laboratory Use.
Description: Promega Streptavidin is purified by affinity chromatography and
is of the highest quality available.
Storage Conditions: Store at –20°C.
19
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