2013 Promega catalogue

Life
Science
Catalog
2013
Worldwide Contact List
Cell Signaling
Maxwell ® 16 System RNA Purification Kits
Product Size Cat.# Price ($)
Low Elution Volume (LEV)
Maxwell ® 16 LEV simplyRNA Cells Kit 48 preps AS1270 Pls. Enq.
Maxwell ® 16 LEV simplyRNA Tissue Kit 48 preps AS1280 Pls. Enq.
Maxwell ® 16 LEV simplyRNA Blood Kit 48 preps AS1310 Pls. Enq.
Maxwell ® 16 Tissue LEV Total RNA Purification Kit 48 preps AS1220 Pls. Enq.
Maxwell ® 16 Cell LEV Total RNA Purification Kit 48 preps AS1225 Pls. Enq.
Maxwell ® 16 Viral Total Nucleic Acid Purification
Kit 48 preps AS1150 Pls. Enq.
Standard Elution Volume (SEV)
Maxwell ® 16 Total RNA Purification Kit 48 preps AS1050 Pls. Enq.
Available Separately Size Cat.# Price ($)
Maxwell ® 16 High Strength LEV Magnetic Rod and
Plunger Bar Adaptor 1 each SP1070 1204
LEV Plungers 50 /pk AS6101 39
Elution Tubes (LEV) 50 /pk AS6201 13
Plungers (SEV) 50 /pk AS5201 28
Elution Tubes (SEV) 50 /pk AS5101 10
AS1270, AS1280, AS1220, AS1225, AS1150, AS1050 For Laboratory Use. AS1310, SP1070,
AS6101, AS6201, AS5201, AS5101 For Research Use Only. Not for Use in Diagnostic
Procedures.
Description: The Maxwell ® 16 LEV simplyRNA Cells Kit and the Maxwell ® 16
LEV simplyRNA Tissue Kit are for use with the Maxwell ® 16 Instrument
configured with the LEV High Strength Magnetic Rod and Plunger Bar
Adaptor. This RNA purification procedure is a simple method with minimal
lysate handling before automated purification on the Maxwell ® 16 Instrument.
The low elution volume is used to generate concentrated high-quality RNA suitable
for use in downstream applications such as quantitative RT-PCR. The kit
provides the reagents for processing the samples and uses prefilled cartridges
for purification, maximizing simplicity and convenience.
The Maxwell ® 16 Total RNA Purification Kit, Maxwell ® 16 Tissue LEV Total
RNA Purification Kit and Maxwell ® 16 Cell LEV Total RNA Purification Kit are
designed for use with the Maxwell ® 16 Instrument in either the standard or low
elution volume (LEV) configuration. The kits provide high-quality,
essentially DNA-free total RNA using novel approaches to selectively remove
genomic DNA prior to automated RNA purification. You get enhanced sensitivity
and improved confidence in your results for quantitative RT-PCR (qRT-PCR),
RT-PCR, cDNA synthesis and other applications.
The simple protocols require adding a cleared lysate to the reagent cartridge.
Simply place the reagent cartridge into the Maxwell ® 16 Instrument, and press
start. Purified RNA is obtained in less than 45 minutes of hands-free instrument
operation. No post-purification treatment with nuclease, cleanup or
concentration is required to achieve superior performance in downstream
applications.
The Maxwell ® 16 Total RNA Purification Kits are General Purpose Medical
Devices (GPR) in the USA. For up-to-date information visit:
www.promega.com/maxwell16/
Features:
• Enjoy Confidence in Your Application Results: Essentially undetectable
contaminating genomic DNA means fewer repeated experiments and
unexplained or variable results.
• Choose Your Sample Type: Flexibility to purify from tissue, cells, blood
and other samples.
• Achieve High Yield and High Concentration: High yields and highconcentration
total RNA result in better performance in gene expression
analysis applications.tions.
simply RNA
TISSUE
CELLS
kidney brain heart lung spleen liver HEK293 HeLa Jurkat
28S
18S
5S
Intact RNA extracted from tissue using the Maxwell ® 16 LEV simplyRNA Tissue Kit. Extracted tissue samples were run on a FlashGel ® System for 5
minutes and signal developed for 15 minutes. The 28S, 18S and 5S are clearly visible indicating intact RNA.
10001TA
Section
Contents
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
5967TB
No detectable cross-contamination. Sixteen purification reactions were
performed using an input of 25mg of mouse liver lysate (odd lanes) or SV
RNA Lysis Buffer alone (even lanes). Panel A. Four-microliter aliquots of
each purified sample were resolved by 1.2% agarose gel electrophoresis
under denaturing conditions. Lane M, RNA Markers (Cat.# G3191).
Panel B. Equivalent volumes (1µl) of each sample were amplified by
endpoint RT-PCR using a primer pair specific for a portion of beta actin
RNA. A total of five microliters of each amplification reaction was analyzed
by 1.2% agarose gel electrophoresis and visualized by ethidium bromide
staining. Lane M, 1kb DNA Ladder (Cat.# G5711).
Table of
Contents
236
For complete and up-to-date product information visit: www.promega.com/catalog