2013 Promega catalogue

Life
Science
Catalog
2013
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Cell Signaling
MultiTox-Glo Multiplex Cytotoxicity Assay
Product Size Cat.# Price ($)
MultiTox-Glo Multiplex Cytotoxicity Assay 10 ml G9270 322
For Laboratory Use.
5 × 10 ml G9271 1195
2 × 50 ml G9272 1791
Description: The MultiTox-Glo Multiplex Cytotoxicity Assay is a sequentialreagent-addition
fluorescent and luminescent assay that measures the relative
number of live and dead cells in cell populations. The MultiTox-Glo Assay
sequentially measures two protease activities; one is a marker of viability, and
the other is a marker of cytotoxicity. The live-cell protease activity is restricted
to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide
substrate (GF-AFC). This substrate enters intact cells, where it is cleaved
by the live cell protease activity to release AFC and generate a fluorescent
signal that is proportional to the number of viable cells. The live-cell protease
becomes inactive upon loss of membrane integrity and leakage into the
surrounding culture medium. A second, luminogenic cell-impermeant peptide
substrate (AAF-aminoluciferin) is used to measure dead-cell protease activity,
which is released from cells that have lost membrane integrity. The liberated
aminoluciferin product is measured as “glow type” luminescence generated by
Ultra-Glo Recombinant Luciferase provided in the assay reagent.
The MultiTox-Glo Assay gives ratiometric, inversely correlated measures of
cell viability and cytotoxicity, which correlate with established methods for
measuring viability and cytotoxicity. The ratio of viable cells to dead cells is
independent of cell number and, therefore, can be used to normalize data.
Having complementary cell viability and cytotoxicity measures reduces errors
associated with pipetting and cell clumping, as well as serving as an internal
control to allow identification of errors resulting from chemical interference
from test compounds or media components.
Features:
• Measure the Number of Live Cells and Dead Cells in Culture:
Sequential-reagent-addition assay with a homogeneous “add-mix-measure”
protocol.
• Normalize Data with a Built-In Internal Control: The ratio of the
number of live cells/number of dead cells is independent of cell number
and can be used to normalize data. This normalization makes results more
comparable well-to-well, plate-to-plate and day-to-day.
• Immediately Identify More False-Positives and False-Negatives:
Independent cell viability and cytotoxicity measurements serve as controls
for each other. If test compounds interfere with one assay chemistry, the
other serves as an internal control.
• Improve your Data: Reduce statistical probability of false-positives (or
false-negatives), and eliminate fluorescence interference issues by luminescence
readout.
Storage Conditions: Store at –20°C, protected from light.
Protocol
MultiTox-Glo Multiplex Cytotoxicity Assay Technical Bulletin
Part#
TB358
Protein Analysis and Complex
Purification
HaloTag ® Mammalian Protein Purification
System
Product Size Cat.# Price ($)
HaloTag ® Mammalian Protein Detection and 1 each G6795 973
Purification System
HaloTag ® Mammalian Protein Purification System 1 each G6790 804
HaloTag ® Mammalian Protein Detection and 1 each G6799 400
Purification System Sample Pack
Available Separately Size Conc. Cat.# Price ($)
HaloTEV Protease 1,000 u 5 u/µl G6601 157
4,000 u 5 u/µl G6602 472
HaloTag ® TMRDirect Ligand 30 μl 0.1 mM G2991 532
Protease Inhibitor Cocktail, 50X 1 ml G6521 89
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The HaloTag ® Mammalian Protein Purification System (Cat.#
G6790) is an optimized kit for purification of HaloTag ® fusion proteins from
mammalian cell culture lysates. HaloTag ® fusion proteins form a highly specific
and covalent bond with the HaloLink Resin. The covalent binding coupled
with the low nonspecific binding of the HaloLink Resin provides superior
purity and recovery of recombinant proteins from cultured mammalian cells,
even at low expression levels. The HaloTag ® Mammalian Protein Detection and
Purification System (Cat.# G6795) also includes HaloTag ® TMRDirect Ligand.
The simple-to-use fluorescent detection of the HaloTag ® fusion facilitates
rapid optimization of expression and purification conditions.
Features:
• Purify More Protein: HaloLink Resin covalently binds >7mg/ml of
HaloTag ® fusion protein (10X more capacity compared to FLAG ® ). Recovery
is highly efficient, commonly >75%.
• Higher Purity: Covalent capture allows extensive and/or stringent washes
without loss of bound protein, resulting in very low (