2013 Promega catalogue

Cell Signaling
Growth Factors
Epidermal Growth Factor, Human,
Recombinant
Product Size Cat.# Price ($)
rhEGF 100 μg G5021 366
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Epidermal Growth Factor, Human, Recombinant (rhEGF) is a
6.2kDa protein that is mitogenic for a variety of mammalian cell types. rhEGF is
produced from recominbant DNA expressed in E. coli.
Activity: rhEGF exhibits an ED 50 value below 0.2ng/ml in the serum-free
BALB/3T3 bioassay using the CellTiter 96 ® Non-Radioactive Cell Proliferation
Assay.
Storage Conditions: Store lyophilized product at –20°C. Rehydrated rhEGF is
stable for 3 months at –20°C. Avoid repeated freeze-thaw cycles.
Protocol
Part#
Epidermal Growth Factor, Human, Recombinant (rhEGF) and Murine
Receptor Grade Protocol
9PIG502
Nerve Growth Factor, 2.5S, Murine
Product Size Cat.# Price ($)
mNGF, 2.5S 100 μg G5141 513
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Murine 2.5S Nerve Growth Factor (2.5S mNGF) mediates phosphorylation
of specific intracellular proteins. Target cells include sympathetic
and sensory neurons and derivatives of nerve cells such as adrenal medulla
pheochromocytoma (PC12) cells. 2.5S mNGF is a 26kDa protein composed
of two identical 118 amino acid chains. Murine 2.5S Nerve Growth Factor is
purified from male mouse submaxillary glands by the method of Bocchini and
Angeletii.
Activity: 2.5S mNGF exhibits an ED 50 value below 2ng/ml using a PC-12
serum-free survival assay.
Storage Conditions: Store lyophilized Murine 2.5S NGF desiccated at –20°C,
where it is stable for at least six months from the date of purchase. Store
reconstituted Murine 2.5S NGF in working aliquots at –20°C, where it is stable
for up to 6 months. Avoid multiple freeze-thaw cycles.
Protocol
Nerve Growth Factor, 2.5S, Murine Product Information
Part#
9PIG514
4
Cell Signaling
Human Brain Derived Neurotrophic Factor
(BDNF)
Product Size Cat.# Price ($)
rhBDNF 5 μg G1491 644
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Brain Derived Neurotrophic Factor, Human, Recombinant
(rhBDNF) is a 27kDa homodimer originally shown to promote the outgrowth of
spinal sensory neurons. rhBDNF is produced from recombinant DNA expressed
in E. coli.
Storage Conditions: Stable for 6 months when stored desiccated at –20°C.
Store reconstituted product in working aliquots at –20°C, where it is stable for
3 months. Avoid multiple freeze-thaw cycles.
Protocol
Brain Derived Neurotrophic Factor, Human, Recombinant
Part#
9PIG149
Human Glial Cell-Line Derived Neurotrophic
Factor (GDNF)
Product Size Cat.# Price ($)
rhGDNF 5 μg G2781 644
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Human Glial Cell-Lined Derived Neurotrophic Factor (rhGDNF) is a
30kDa homodimer consisting of two disulfide-linked, 134 amino acid subunits.
GDNF promotes dopamine uptake and survival of midbrain neurons and is
also a survival factor for developing motor neurons, purified rat embryo spinal
neurons and nodose sensory neurons. rhGDNF is produced from recombinant
DNA expressed in E. coli.
Storage Conditions: Stable for 6 months when stored desiccated at –20°C.
Store reconstituted product in working aliquots at –20°C, where it is stable for
3 months from date of purchase. Avoid multiple freeze-thaw cycles.
Protocol
Glial Cell Line-Derived Neurotrophic Factor, Human, Recombinant
Protocol
Part#
9PIG278
rhFGF, Basic
Product Size Cat.# Price ($)
rhFGF, Basic 25 μg G5071 652
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Fibroblast Growth Factor, Basic, Human, Recombinant (rhFGF,
Basic), is a 17.5kDa polypeptide containing 154 amino acids. It induces proliferation
of multiple types of cells in vitro and demonstrates potent angiogenic
activity in vivo. rhFGF, Basic, is produced from recombinant DNA expressed in
E. coli.
Protocol
Fibroblast Growth Factor, Basic, Human, Recombinant Protocol
rhIGF-I
Part#
9PIG507
Product Size Cat.# Price ($)
rhIGF-I 25 μg G5111 808
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Insulin-Like Growth Factor-I, Human, Recombinant (rhIGF-I), is a
7.6kDa protein containing 70 amino acid residues. It stimulates the proliferation
of a wide range of cell types. rhIGF-I is a highly purified, biologically active,
recombinant molecule, produced in E. coli.
Protocol
Insulin-like Growth Factor I, Human, Recombinant Protocol
rhTNF-α
For complete and up-to-date product information visit: www.promega.com/catalog
Part#
9PIG511
Product Size Cat.# Price ($)
rhTNFα 10 μg G5241 556
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Tumor Necrosis Factor-α, Human, Recombinant (rhTNF-α), is a
pleiotropic cytokine produced predominantly by activated monocytes/macrophages.
Biological effects of this molecule include induction of apoptosis, cytolysis
or cytostasis of tumor cells, activation of polymorphonuclear leukocytes,
antiviral activity and induction of IL-1 or colony-stimulating factor expression.
rhTNF-α is a 17kDa protein containing 157 amino acid residues produced from
a recombinant DNA expressed in E. coli.
Protocol
Tumor Necrosis Factor alpha, Human, Recombinant Protocol
Part#
9PIG524
61
Section
Contents
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