2013 Promega catalogue

Cell Signaling
Unmethylated Lambda DNA
Product Size Cat.# Price ($)
Unmethylated Lambda DNA 250 μg D1521 109
For Laboratory Use.
Description: Unmethylated cl857 Sam7 Lambda DNA (48,502bp) is isolated
from infected GM119, an E. coli strain lacking both the dam and dcm methylase
activities. Unmethylated Lambda DNA is used as a substrate for restriction
enzymes sensitive to DNA methylation.
Features:
• Unmethylated Substrate: Use as a substrate for methylation-sensitive
restriction enzymes.
Storage Conditions: Store at –20°C.
pBR322 Vector
Product Size Conc. Cat.# Price ($)
pBR322 Vector 10 μg 1 µg/µl D1511 89
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The plasmid pBR322 Vector (4,361bp) carries the genes for
tetracycline and ampicillin resistance. pBR322 DNA digests typically are used
as molecular weight size markers in gel analysis of nucleic acids.
Storage Conditions: Store at –20°C.
PvuII
2066
ori
StyI 1369
pBR322
Vector
(4,361bp)
PstI 3611
PvuI 3736
ScaI 3846
Amp r
EcoRI 4359
HindIII 29
EcoRV 187
BamHI 375
Tet r SphI 566
SalI 651
BstZI 939
0270VA05_2A
φΦX174, RF DNA
Product Size Conc. Cat.# Price ($)
ΦX174, RF DNA 50 μg 1 µg/µl D1531 172
For Laboratory Use.
Description: The icosahedral bacteriophage ΦX174 replicative form (RF) is a
double-stranded circular DNA molecule of 5,386 bases. Restriction enzymedigested
ΦX174 DNA generates molecular weight size markers used in gel
analysis of nucleic acids. ΦX174 DNA is often used in the assays of restriction
enzymes for the presence of nickase activity.
Storage Conditions: Store at –20°C.
StuI 4489
AccI 3545
AsuI 5042
HpaI 5002
SacII 2863
HpaI 28 XhoI 163
PstI 1 MluI 222
DraI 327
φX174
(5,386bp)
SinI 2782
MluI 2147
K562 DNA High Molecular Weight
AsuI 978
AccI 1193
HpaI 1292
DraI 1406
Product Size Cat.# Price ($)
K562 DNA High Molecular Weight 30 μg DD2011 178
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: K562 DNA is purified from a subculture of the human chronic
myelogenous leukemia cell line. K562 DNA serves as a control for most steps
of the single-locus probe analysis procedure. The DNA also can be used as a
reference for determining fragment sizes of VNTR alleles following appropriate
restriction digestion. K562 fragment sizes obtained may vary slightly due to
interlaboratory differences in protocols and methods of analysis.
Concentration: 0.4–1.0μg/μl.
Storage Conditions: Store at –20°C. Always avoid multiple freeze-thaw
cycles or exposure to frequent temperature changes. These fluctuations can
greatly alter product stability.
0271VA04_1A
2
Biochemicals and Labware
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