2013 Promega catalogue

Life
Science
Catalog
2013
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Cell Signaling
Cell ID System
Product Size Cat.# Price ($)
Cell ID System
50 reactions G9500 Pls. Enq.
Available Separately Size Cat.# Price ($)
Water, Amplification Grade
6,250 μl DW0991 Pls. Enq.
Internal Lane Standard 600 150 μl DG1071 362
DW0991, DG1071 For Laboratory Use. G9500 For Research Use Only. Not for Use in Diagnostic
Procedures.
Description: The Cell ID System provides the reagents required for
successful and simple identification and authentication of human cell lines and
detection of intra-species cell line cross-contamination. The Cell ID System
uses short tandem repeat (STR) analysis of specific loci in the human genome
through co-amplification and three-color detection of ten loci (nine STR loci and
Amelogenin for gender identification), including D21S11, TH01, TPOX, vWA,
Amelogenin, CSF1PO, D16S539, D7S820, D13S317 and D5S818. All ten loci
are amplified simultaneously in a single tube. The Cell ID System includes
a hot-start Taq DNA polymerase system in an enzyme mix for convenient
room-temperature reaction assembly. Alleles, along with the allelic ladder and
an internal lane standard that assists in assigning sizes for each locus, are
resolved by capillary electrophoresis (CE) in a single injection, and CE data are
analyzed using allele-calling software to generate a genetic profile of the cell line.
These loci collectively provide a genetic profile with a random match probability
of 1 in 2.92 × 10 9 .
The Cell ID System is designed specifically for use with the Applied Biosystems
® 3130 and 3130xl Genetic Analyzers, ABI PRISM ® 3100 and 3100-Avant
Genetic Analyzers and ABI PRISM ® 310 Genetic Analyzer.
Dye matrix generation is required on initial setup and is performed using the
PowerPlex ® Matrix Standards, 310 (Cat.# DG4640), or PowerPlex ® Matrix
Standards, 3100/3130 (Cat.# DG4650).
Features:
• Improved “Gold Standard” STR Analysis: The Cell ID System
includes all loci used in the original PowerPlex ® 1.2 System plus the
D21S11 locus for improved power of discrimination.
• Simplified Data Interpretation: The Cell ID System allows
co-amplification of ten loci to accurately discriminate between human cell
lines without using a full forensic profile and includes allelic ladders for all
ten loci to simplify data interpretation.
• Simplified Amplification Setup: Inclusion of a hot-start Taq DNA
polymerase allows room-temperature reaction assembly.
• Internal Lane Standard 600: This marker offers the greatest precision
available for DNA typing. It is designed for use in each capillary injection to
increase the precision of your analyses.
• Automatic Assignment of Genotypes: To make genotyping easier and
more accurate, panels and bins files are available for download and are
required for use with GeneMapper ® ID software. Use of GeneMapper ® 4.0
software requires custom bin generation, and procedures can be found on
the Cell ID System Analysis Support Software page at: www.promega.
com/resources/tools/cell-id-system-analysis-support-software/
Storage Conditions: Store at –20°C. The Primer Pair Mix, Allelic Ladder and
Internal Lane Standard are light-sensitive and should be stored protected from
light.
Protocol
Cell ID System Technical Manual
Fluorescein:
JOE:
TMR:
A
D5S818
vWA
TH01
D21S11
D13S317 D7S820 D16S539 CSF1PO
TPOX
Part#
TM074
CXR:
Size (bp) 100 200 300 400
A = Amelogenin
Allele ranges for the Cell ID System. STR fragments amplified by
the Cell ID System are labeled with different dyes and are separated
by capillary electrophoresis based on size. A size standard is included in
each sample to determine the size of the Cell ID-amplified fragments.
Fluorescein-labeled loci are shown in dark gray, JOE-labeled loci are
shown in gray and TMR-labeled loci are shown in white. The CXR-labeled
Internal Lane Standard 600 fragments are represented by black bars.
7426MA
Section
Contents
Table of
Contents
338
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