2013 Promega catalogue

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Life Science Catalog <strong>2013</strong> Worldwide Contact List Section Contents Cell Signaling HaloTag ® Fusion (N-Terminal) Mammalian Expression Vectors Product Size Cat.# Price ($) pHTN HaloTag ® CMV-neo Vector 20 μg G7721 363 pFN28A HaloTag ® CMV-neo Flexi ® Vector 20 μg G8441 405 pFN28K HaloTag ® CMV-neo Flexi ® Vector 20 μg G8451 405 pFN21A HaloTag ® CMV Flexi ® Vector 20 μg G2821 405 pFN21K HaloTag ® CMV Flexi ® Vector 20 μg G2831 405 pFN22A HaloTag ® CMVd1 Flexi ® Vector 20 μg G2841 405 pFN22K HaloTag ® CMVd1 Flexi ® Vector 20 μg G2851 405 pFN23A HaloTag ® CMVd2 Flexi ® Vector 20 μg G2861 405 pFN23K HaloTag ® CMVd2 Flexi ® Vector 20 μg G2871 405 pFN24A HaloTag ® CMVd3 Flexi ® Vector 20 μg G2881 405 pFN24K HaloTag ® CMVd3 Flexi ® Vector 20 μg G2981 405 Available Separately Size Cat.# Price ($) HaloTag ® Cloning Starter System 1 each G6050 580 HaloTag ® Flexi ® Vectors-CMV Deletion Series Sample Pack 9 × 2 μg G3780 340 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The HaloTag ® vectors are are designed for expression of N-terminal-tagged HaloTag ® fusion proteins in mammalian cells. Once expressed, the HaloTag ® fusion protein may be used for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag ® Ligands. In addition, the HaloTag ® fusion protein can be purified or pulled down as a complex with its protein partners. We offer two types of HaloTag ® fusion vectors to accommodate your cloning preferences: • pHT Vector Series: Simple Multiple Cloning Site (MCS) plasmids for traditional cloning. • pF Vector Series: Flexi ® Vector Cloning System—a directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi ® Vectors without the need to resequence. Note: Flexi ® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi ® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert. Features: • Versatility: You can choose between a variety of initial applications (e.g., bacterial protein, mammalian, or cell-free protein expression) and then transfer to others as required. • Time Savings: Efficient transfer allows for direct use of recombinant clones, minimizing time wasted screening background colonies. • Enhanced Productivity: Adaptable to high-throughput formats for large screening projects. • Easy Access: No licensing fees or complicated transfer restrictions. Storage Conditions: Store vectors at –20°C. Protocol pHTN HaloTag ® CMV-neo Vector Product Information pFN28A HaloTag CMV-neo Flexi Vector Product Information pFN28K HaloTag ® CMV-neo Flexi ® Vector Product Information pFN21A HaloTag ® CMV Flexi ® Vector Protocol pFN21K HaloTag ® CMV Flexi ® Vector Protocol pFN22A HaloTag ® CMVd1 Flexi ® Vector Protocol pFN22K HaloTag ® CMVd1 Flexi ® Vector Protocol pFN23A HaloTag ® CMVd2 Flexi ® Vector Protocol pFN23K HaloTag ® CMVd2 Flexi ® Vector Protocol Part# 9PIG772 9PIG844 9PIG845 9PIG282 9PIG283 9PIG284 9PIG285 9PIG286 9PIG287 Protocol pFN24A HaloTag ® CMVd3 Flexi ® Vector Protocol pFN24K HaloTag ® CMVd3 Flexi ® Vector Protocol Relative Mammalian Protein Expression Levels for HaloTag ® Flexi ® Vectors. Vector Name Cat.# Expression Level* pFC14A HaloTag ® CMV Flexi ® Vector pFC14K HaloTag ® CMV Flexi ® Vector pFC15A HaloTag ® CMVd1 Flexi ® Vector pFC15K HaloTag ® CMVd1 Flexi ® Vector pFC16A HaloTag ® CMVd2 Flexi ® Vector pFC16K HaloTag ® CMVd2 Flexi ® Vector pFC17A HaloTag ® CMVd3 Flexi ® Vector pFC17K HaloTag ® CMVd3 Flexi ® Vector pFN21A HaloTag ® CMV Flexi ® Vector pFN21K HaloTag ® CMV Flexi ® Vector pFN22A HaloTag ® CMVd1 Flexi ® Vector pFN22K HaloTag ® CMVd1 Flexi ® Vector pFN23A HaloTag ® CMVd2 Flexi ® Vector pFN23K HaloTag ® CMVd2 Flexi ® Vector pFN24A HaloTag ® CMVd3 Flexi ® Vector pFN24K HaloTag ® CMVd3 Flexi ® Vector pFC27A HaloTag ® CMV-neo Flexi ® Vector pFC27K HaloTag ® CMV-neo Flexi ® Vector pFN28A HaloTag ® CMV-neo Flexi ® Vector pFN27K HaloTag ® CMV-neo Flexi ® Vector G9651 G9661 G1611 G1601 G1591 G1571 G1551 G1321 G2821 G2831 G2841 G2851 G2861 G2871 G2881 G2981 High High Medium Medium Low Low Ultra-Low Ultra-Low High High Medium Medium Low Low Ultra-Low Ultra-Low G8421 High 1 G8431 High 2 G8441 High 1 Part# 9PIG288 9PIG298 G8451 High 2 9161LB 1 This vector confers ampicillin resistance for Flexi ® cloning, and the neomycin (G418) selection cassette affords antibiotic selection of stable cell lines expressing the HaloTag ® fusion protein. 2 This vector confers kanamycin resistance for Flexi ® cloning, and the neomycin (G418) selection cassette affords antibiotic selection of stable cell lines expressing the HaloTag ® fusion protein. *Expression level depends on the cell type and the protein fused to HaloTag ® protein. Table of Contents 274 For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling Inducible T7-Driven Flexi ® Vectors for E. coli Expression Product Size Cat.# Price ($) pH6HTN His 6 HaloTag ® T7 Vector 20 μg G7971 363 pH6HTC His 6 HaloTag ® T7 Vector 20 μg G8031 363 pF1A T7 Flexi ® Vector 20 μg C8441 435 pF1K T7 Flexi ® Vector 20 μg C8451 435 pFN18A HaloTag ® T7 Flexi ® Vector 20 μg G2751 405 pFN18K HaloTag ® T7 Flexi ® Vector 20 μg G2681 405 pFN19A HaloTag ® T7 SP6 Flexi ® Vector 20 μg G1891 405 pFN19K HaloTag ® T7 SP6 Flexi ® Vector 20 μg G1841 405 pFC20A HaloTag ® T7 SP6 Flexi ® Vector 20 μg G1681 405 pFC20K HaloTag ® T7 SP6 Flexi ® Vector 20 μg G1691 405 pFN29A His 6 HaloTag ® T7 Flexi ® Vector 20 μg G8261 405 pFN29K His 6 HaloTag ® T7 Flexi ® Vector 20 μg G8331 405 pFC30A His 6 HaloTag ® T7 Flexi ® Vector 20 μg G8321 405 pFC30K His 6 HaloTag ® T7 Flexi ® Vector 20 μg G8381 405 pFN2A (GST) Flexi ® Vector 20 μg C8461 489 pFN2K (GST) Flexi ® Vector 20 μg C8471 489 pFN6A (HQ) Flexi ® Vector 20 μg C8511 489 pFN6K (HQ) Flexi ® Vector 20 μg C8521 489 pFC7A (HQ) Flexi ® Vector 20 μg C8531 489 pFC7K (HQ) Flexi ® Vector 20 μg C8541 489 For Research Use Only. Not for Use in Diagnostic Procedures. Description: These Flexi ® vectors are designed specifically for expression of proteins in E. coli from the T7 RNA polymerase promoter with or without the addition of purification tags. Express levels can vary from protein to protein, but in general the N-terminal HaloTag ® fusion protein (e.g., pFN18A/K) can increase expression by increasing solubility of the expressed protein. Proteins may be purified using the HaloTag ® fusion with the HaloTag ® Protein Purification System (Cat.# G6280). Expression may be confirmed through cell-free expression with the S30 T7 High-Yield Protein Expression System (Cat.# L1110). The Flexi ® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi ® Vectors without the need to resequence. Note: Flexi ® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi ® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert. Features: • Versatility: You can choose between a variety of initial applications (e.g., bacterial protein, mammalian, or cell-free protein expression) and then transfer to others as required. • Time Savings: Efficient transfer allows for direct use of recombinant clones, minimizing time wasted screening background colonies. • Enhanced Productivity: Adaptable to high-throughput formats for large screening projects. • Easy Access: No licensing fees or complicated transfer restrictions. Storage Conditions: Store vectors at –20°C. Protocol pH6HTN His6HaloTag ® T7 Vector Product Information pH6HTC His 6 HaloTag ® T7 Vector Product Information pF1A T7 Flexi ® Vector Product Information pF1K T7 Flexi ® Vector Product Information pFN18A HaloTag ® T7 Flexi ® Vector Protocol pFN18K HaloTag ® T7 Flexi ® Vector Protocol pFN19A HaloTag ® T7 SP6 Flexi ® Vector Protocol pFN19K HaloTag ® T7 SP6 Flexi ® Vector Protocol pFC20A HaloTag ® T7 SP6 Flexi ® Vector Protocol pFC20K HaloTag ® T7 SP6 Flexi ® Vector Protocol pFN29A His6HaloTag ® T7 Flexi ® Vector Product Information pFN29K His6HaloTag ® T7 Flexi ® Vector Product Information pFC30A His6HaloTag ® T7 Flexi ® Vector Product Information pFC30K His6HaloTag ® T7 Flexi ® Vector Product Information pFN2A (GST) Flexi ® Vector Product Information pFN2K (GST) Flexi ® Vector Product Information pFN6A (HQ) Flexi ® Vector Product Information pFN6K (HQ) Flexi ® Vector Product Information pFC7A (HQ) Flexi ® Vector Product Information pFC7K (HQ) Flexi ® Vector Product Information Part# 9PIG797 9PIG803 9PIC844 9PIC845 9PIG275 9PIG268 9PIG189 9PIG184 9PIG168 9PIG169 9PIG826 9PIG833 9PIG832 9PIG838 9PIC846 9PIC847 9PIC851 9PIC852 9PIC853 9PIC854 15 Protein Expression and Analysis Section Contents For complete and up-to-date product information visit: www.promega.com/catalog 275 Table of Contents
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Life Science CATALOG 2013 Table of
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Cell Signaling Table of Contents Pr
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Cell Signaling 1 Biobanking 1 DNA E
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Cell Signaling DNA Extraction for B
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Cell Signaling Wizard ® Genomic DN
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Cell Signaling QuantiFluor RNA Syst
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Cell Signaling Sample ID and Mixed
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Cell Signaling 2 Biochemicals and L
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling SDS Solution, Molecu
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Magnetic Stands and
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Cell Signaling 3 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 8 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling CellTiter-Glo ® One
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Cell Signaling Fluorescent Cell Via
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Cell Signaling CellTiter-Blue ® Ce
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Cell Signaling ApoTox-Glo Triplex A
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Cell Signaling CytoTox-Fluor Cytoto
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 4 Cell Signaling 4 G
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Kinase Assay
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Cell Signaling Kinase Enzyme System
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Cell Signaling ProFluor ® PKA Assa
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Cell Signaling PepTag ® Non-Radioa
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Cell Signaling Product Size Cat.# P
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Cell Signaling kDa 98 - 64 - 50 - 3
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Cell Signaling ProFluor ® Ser/Thr
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Cell Signaling 5 Cloning and DNA Ma
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling RNA Markers Product
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Cell Signaling AatII 37 Product Siz
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Cell Signaling BstEII 60 Product Si
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Cell Signaling HhaI 37 Product Size
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Cell Signaling NdeI 37 Product Size
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Cell Signaling SnaBI 37 Product Siz
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Cell Signaling Alkaline Phosphatase
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Cell Signaling T4 DNA Polymerase Pr
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Cell Signaling Kinases and DNA Labe
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Cell Signaling Additional Enzymes S
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Cell Signaling Subcloning Tools and
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Cell Signaling pALTER ® -MAX Vecto
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Cell Signaling pGEM ® -7Zf(+/-) Ve
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Cell Signaling pSP72 Vector Product
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Cell Signaling 6 DNA and RNA Purifi
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep Blood gDNA
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Cell Signaling Wizard ® SV 96 Geno
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Cell Signaling Fixed-Tissue Genomic
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Cell Signaling Maxwell ® 16 System
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Cell Signaling PureYield Plasmid Mi
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Cell Signaling Wizard ® Plus Minip
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Cell Signaling Wizard ® SV 96 and
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Cell Signaling RNA Purification Rel
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA and RNA Quantita
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Cell Signaling Magnetic Stands and
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Cell Signaling Vacuum Manifolds and
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Cell Signaling 7 Drug Discovery 7 D
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling GloResponse Lucifera
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Cell Signaling DPPIV-Glo Protease A
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Cell Signaling Calpain-Glo Protease
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Cell Signaling 8 Epigenetics 8 DNA
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Cell Signaling DNA Purification Tec
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Cell Signaling Luciferase-Based Met
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Cell Signaling GoTaq ® Long PCR Ma
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Cell Signaling DUB-Glo Protease Ass
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Cell Signaling HaloTag ® Protein P
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Cell Signaling 9 Genetic Identity 9
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Cell Signaling DNA IQ System Produc
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Cell Signaling DNA IQ Casework Pro
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Cell Signaling STR Analysis for For
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Cell Signaling PowerPlex ® Y23 Sys
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® S5 Syst
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Cell Signaling GenePrint ® Fluores
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Cell Signaling 10 Imaging and Immun
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling NGF E max ® ImmunoA
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Cell Signaling Anti-ACTIVE ® CaM K
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Cell Signaling A. Anti-ACTIVE ® MA
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling Anti-NGF mAb Product
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Cell Signaling Anti-TGFβ 1 pAb Pro
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Cell Signaling Horseradish Peroxida
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Cell Signaling ViviRen In Vivo Reni
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Cell Signaling 11 Industrial and En
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Cell Signaling QuantiLum ® Recombi
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12 Instruments 12 Luminometers 218
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
Page 227 and 228: GloMax ® -Multi Jr Single-Tube Mul
Page 229 and 230: Maxwell ® CSC System for IVD Use M
Page 231 and 232: Maxwell ® Research Systems Maxwell
Page 233 and 234: Maxwell ® 16 Flexi Method Firmware
Page 235 and 236: Cell Signaling 13 Molecular Diagnos
Page 237 and 238: Cell Signaling Maxwell ® CSC Servi
Page 239 and 240: Cell Signaling Maxwell ® 16 System
Page 241 and 242: Cell Signaling Maxwell ® 16 Flexi
Page 243 and 244: Cell Signaling Deoxynucleotide Trip
Page 245 and 246: Cell Signaling 14 PCR 14 Hot-Start
Page 247 and 248: Cell Signaling Routine PCR GoTaq ®
Page 249 and 250: Cell Signaling Pfu DNA Polymerase d
Page 251 and 252: Cell Signaling GoTaq ® Real-Time q
Page 253 and 254: Cell Signaling StemElite Human Panc
Page 255 and 256: Cell Signaling Reverse Transcriptio
Page 257 and 258: Cell Signaling AMV Reverse Transcri
Page 259 and 260: Cell Signaling PCR Cloning pGEM ®
Page 261 and 262: Cell Signaling 15 Protein Expressio
Page 263 and 264: Cell Signaling TnT ® Quick Coupled
Page 265 and 266: Cell Signaling TnT ® T7 Quick for
Page 267 and 268: Cell Signaling Canine Pancreatic Mi
Page 269 and 270: Cell Signaling E. coli S30 Extract
Page 271 and 272: Cell Signaling Mass Spectrometry An
Page 273 and 274: Cell Signaling Endoproteinase Lys-C
Page 275 and 276: Cell Signaling Protein Labeling and
Page 277: Cell Signaling HaloTag ® Fusion (C
Page 281 and 282: Cell Signaling ECL Western Blotting
Page 283 and 284: Cell Signaling 16 Protein Purificat
Page 285 and 286: Cell Signaling HaloTag ® Protein P
Page 287 and 288: HT Cell Signaling HaloTag ® Mammal
Page 289 and 290: Cell Signaling HaloLink Protein Arr
Page 291 and 292: Cell Signaling FastBreak Cell Lysis
Page 293 and 294: Cell Signaling MagneHis Protein Pur
Page 295 and 296: Cell Signaling Streptavidin Product
Page 297 and 298: Cell Signaling Protein Purification
Page 299 and 300: Cell Signaling 17 Reporter Assays a
Page 301 and 302: Cell Signaling NanoLuc Luciferase
Page 303 and 304: Cell Signaling Chroma-Glo Luciferas
Page 305 and 306: Cell Signaling ONE-Glo + Tox Lucife
Page 307 and 308: Cell Signaling Luciferase Assay Sys
Page 309 and 310: Cell Signaling ViviRen Live Cell Su
Page 311 and 312: Cell Signaling Reporter Vectors and
Page 313 and 314: Cell Signaling Promoterless Renilla
Page 315 and 316: Cell Signaling pmirGLO Dual-Lucifer
Page 317 and 318: Cell Signaling pGL2 Luciferase Repo
Page 319 and 320: Cell Signaling Reporter Vector Sequ
Page 321 and 322: Cell Signaling pCAT ® 3 Vectors Pr
Page 323 and 324: Cell Signaling ViviRen In Vivo Reni
Page 325 and 326: Cell Signaling 18 RNA Analysis 18 I
Page 327 and 328: Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 19 Stem Cell Researc
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Cell Signaling Cell ID System Fluor
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Cell Signaling StemElite Human Panc
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Cell Signaling 20 STR Analysis 20 C
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Cell Signaling PowerPlex ® 16 HS S
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Cell Signaling StemElite ID System
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Cell Signaling 21 Vectors 21 Bacter
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Cell Signaling Mammalian Expression
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Cell Signaling pCI-neo Mammalian Ex
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Cell Signaling pSV-β-Galactosidase
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22 Index 22 Index: A-Z 354 Index by
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Index: A-Z Click on name to go to p
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Index by Catalog Number Click on pa
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1S11 TH01 D3S1358 FGA TPOX D8S1179
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