2013 Promega catalogue

Life
Science
Catalog
2013
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Cell Signaling
Beta-Glo ® Assay System
Product Size Cat.# Price ($)
Beta-Glo ® Assay System 10 ml E4720 154
For Research Use Only. Not for Use in Diagnostic Procedures.
100 ml E4740 1062
10 × 100 ml E4780 Pls. Enq.
Description: The Beta-Glo ® Assay System is a homogeneous method of
quantitating β-galactosidase expression in mammalian cells. The system
provides a bright luminescent signal that is stable over several hours in
commonly used cell culture medium without prior sample processing. The
homogeneous assay procedure involves the addition of a single reagent directly
to cells cultured in serum-supplemented medium. Throughput rates of several
thousand samples per hour may be achieved with high reproducibility under
standard laboratory conditions.
Features:
• Bright Luminescent Signal: Quantitate with confidence using lowvolume
formats or in samples with low-level expression.
• Homogeneous Format: Perform fewer steps. Add a single reagent
directly to cells in growth medium.
• Stable Signal: Obtain flexibility and convenience when processing
multiple plates.
• Convenient: Achieve optimal assay performance at room temperature.
• Flexible: Read the luminescent signal using any luminometer. Injectors are
not required.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
Protocol
Beta-Glo ® Assay System Technical Manual
Log Luminescence (RLU)
6
5
4
3
2
1
0
Control Protein 1 Protein 2
Part#
TM239
6042MA
CAT Reporter Systems
CAT Enzyme Assay System
Product Size Cat.# Price ($)
CAT Enzyme Assay System 50 reactions E1000 203
Available Separately Size Conc. Cat.# Price ($)
Chloramphenicol Acetyltransferase 100 u 10–14 u/µl E1051 82
n-Butyryl CoA 255 μl 5 mg/ml E1061 103
Reporter Lysis 5X Buffer 30 ml E3971 108
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The CAT Enzyme Assay System offers two alternative methods
for monitoring CAT enzyme activity in transfected cells: liquid scintillation
counting (LSC) and thin layer chromatography (TLC). Either the LSC or TLC
assays can be performed using the same cell extract. The TLC-based assay
is less sensitive and more time-consuming to perform than the LSC assay but
is useful as a visual confirmation of assay results. The resolved TLC reaction
products are detected by autoradiography or phosphorimaging analysis.
Chloramphenicol Acetyltransferase (CAT), encoded by a bacterial drug-resistance
gene, catalyzes the transfer of an acetyl group from acetyl-CoA to the
3´-hydroxy position of chloramphenicol. The enzyme is suitable as a standard in
CAT assays of crude cell extracts. One unit is defined as the amount of enzyme
required to transfer 1nmol of butyrate or acetate to chloramphenicol in one
minute at 37°C.
n-Butyryl CoA is suitable for use in the chloramphenicol acetyltransferase (CAT)
reaction. Transfer of the n-butyryl moiety to chloramphenicol by the CAT
enzyme allows enzyme activity to be monitored using liquid scintillation counting
or thin layer chromatography formats.
Features:
• Fast: The assay is performed in as little as 2–3 hours.
• Linear: The LSC assay is linear for three orders of magnitude of enzyme
activity.
• Sensitive: As little as 3 × 10 –4 units (2pg) of CAT can be detected.
• Robust: Reporter Lysis Buffer allows luciferase, CAT and β-galactosidase
assays to be performed from the same cell extract.
Storage Conditions: Reporter Lysis 5X Buffer may be stored at room
temperature. Store other system components at –20°C.
Protocol
CAT Enzyme Assay System with Reporter Lysis Buffer Technical
Bulletin
Part#
TB084
β-galactosidase activity determined using the Beta-Glo ® Assay
System with a yeast two-hybrid system. Image kindly provided by Dr.
Brad Hook, Ph.D., University of Wisconsin, Madison.
Section
Contents
Table of
Contents
306
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