2013 Promega catalogue

Cell Signaling
Wizard ® SV 96 Genomic DNA Purification
System
Product Size Cat.# Price ($)
Wizard ® SV 96 Genomic DNA Purification
System
1 × 96 preps A2370 429
4 × 96 preps A2371 1568
Available Separately Size Conc. Cat.# Price ($)
Wizard ® SV Lysis Buffer 50 ml Z3052 66
Column Wash Solution (CWA) 185 ml A1311 156
Nuclei Lysis Solution 50 ml A7941 51
EDTA, 0.5M (pH 8.0), Molecular Biology
Grade 100 ml V4231 33
RNase A Solution 1 ml 4 mg/ml A7973 415
Wizard ® SV 96 Binding Plates 10 pack A2271 1450
Z3052, A1311 For Laboratory Use. A2370, A2371, A7941, V4231, A7973, A2271 For Research
Use Only. Not for Use in Diagnostic Procedures.
Description: The Wizard ® SV 96 Genomic DNA Purification System provides
a high-throughput, membrane-based technique for consistent preparation of
genomic DNA from cultured cells and tissue, including mouse tails. Amplifiable
genomic DNA can be isolated from up to 5 × 10 6 cells, 20mg of tissue or up to
1.2cm of a mouse tail tip without a centrifugation clearing step.
With the Wizard ® SV Genomic DNA purification system, genomic DNA is
purified from cell lysates using 96-well vacuum filtration. Washing the bound
DNA requires no disassembly of the manifold, and filtrate waste products
are delivered directly to a vacuum trap, eliminating the need to empty waste
collection trays.
The Wizard ® SV Genomic DNA Purification System is designed for use either in
a manual format or with Beckman Coulter or PerkinElmer automated
instruments.
Features:
• Improve Productivity: Obtain genomic DNA from mouse tails in
45–60 minutes, genomic DNA from cultured cells in 30 minutes. No spins
required.
• Achieve High Yield: Purify 20–30μg of DNA per prep from 1.2cm of
mouse tail.
• Gain Confidence in Applications: Purified DNA ready for amplification.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at 22–25°C.
MagneSil ® ONE, Fixed Yield Blood Genomic
System
Product Size Cat.# Price ($)
MagneSil ® ONE, Fixed Yield Blood Genomic 1 × 96 preps MD1370 464
System
Collection Plates (4-pack) 1 each A9161 24
Available Separately Size Cat.# Price ($)
Lysis Buffer, Blood 160 ml MD1392 162
Anti-Foam Reagent 300 μl MD1431 49
MagneSil ® PMPs-Fixed Yield 25 ml MD1451 376
Alcohol Wash, Blood 120 ml MD1412 93
Elution Buffer, Blood 45 ml MD1421 49
MD1370, MD1431, MD1451, MD1412 For Laboratory Use. MD1392, A9161, MD1421 For
Research Use Only. Not for Use in Diagnostic Procedures.
Description: The MagneSil ® ONE, Fixed Yield Blood Genomic System purifies
1μg of DNA (+/– 50%) from 60μl of anti-coagulated whole blood.
Purification of a “fixed yield” of DNA eliminates the need to quantitate and
normalize concentrations postpurification. The highly pure DNA isolated is
suitable for use in PCR, multiplex PCR and SNP genotyping applications.
Walkaway automation is available on the Beckman Coulter Biomek ® FX in a
96-well format. Process 96 samples in about 1 hour with no hands-on time
following robot setup.
Features:
• Improve Productivity: Use walkaway automation to extract genomic DNA
and eliminate DNA quantitation prior to PCR.
• Achieve Consistent Results: Obtain 1μg (fixed yield) of highly pure DNA
from 60μl of blood.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at 20–25°C.
Protocol
MagneSil ® ONE, Fixed Yield Blood Genomic System Technical
Bulletin
Part#
TB313
6
DNA and RNA Purification
Protocol
Wizard ® SV 96 Genomic DNA Purification System Technical Bulletin
Part#
TB303
Section
Contents
For complete and up-to-date product information visit: www.promega.com/catalog
129
Table of
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