2013 Promega catalogue

Life
Science
Catalog
2013
Worldwide Contact List
Cell Signaling
Renilla Luciferase Reporter Systems
Renilla-Glo Luciferase Assay System
Product Size Cat.# Price ($)
Renilla-Glo Luciferase Assay System 10 ml E2710 301
For Research Use Only. Not for Use in Diagnostic Procedures.
100 ml E2720 1659
10 × 100 ml E2750 Pls. Enq.
Description: The Renilla-Glo Luciferase Assay System is a single-addition
reagent that generates a glow-type signal with Renilla luciferase. When
reconstituted, it has the capacity to lyse cells, reduce the autoluminescence of
the coelenterazine substrate, and produce a stable signal (i.e., half-life greater
than 60 minutes at 22°C).
Features:
• Simplify Your Assay Optimization: Add-and-read simplicity for a Renilla
luciferase reporter system.
• Improve Assay Precision: No need for separate lysis and reagent
injection steps.
• Get a Brighter, Longer-Lasting Signal: Extended bright light output is
optimized for batch and continuous-process handling.
• Reduced Autoluminescence: Low background formulation offers
increased sensitivity.
Storage Conditions: Store at –20°C.
Protocol
Renilla-Glo Luciferase Assay System Technical Manual
Renilla Luciferase Assay System
Part#
TM329
Product Size Cat.# Price ($)
Renilla Luciferase Assay System 100 assays E2810 274
1,000 assays E2820 1508
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Renilla Luciferase Assay System is designed to provide a fast and
sensitive method of detecting the luciferase from sea pansy (Renilla reniformis).
The system is a convenient alternative to firefly (Photinus pyralis) reporter
systems and is designed to yield reliable, linear results for a concentration
range over 7 orders of magnitude. The Renilla Luciferase Assay System has
been formulated with a proprietary composition that significantly reduces the
effect of coelenterazine autoluminescence when compared to other reagents,
making the reagent orders of magnitude more sensitive than published
methods. This system enables measurements with wildtype and the synthetic
hRluc genes for primary expression or internal normalization measurements of
gene expression.
Features:
• Reduced Autoluminescence: Low background, increased sensitivity.
• Sensitive: 10 –19 moles of Renilla luciferase detectable.
• Linear: Linear range extending 7 logs.
• Unique: The first independent assay system for Renilla luciferase.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store the Renilla Luciferase Assay System at –20°C.
Protocol
Renilla Luciferase Assay System Technical Manual
Part#
TM055
EnduRen Live Cell Substrate
Product Size Cat.# Price ($)
EnduRen Live Cell Substrate 0.34 mg E6481 213
3.4 mg E6482 1372
34 mg E6485 Pls. Enq.
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: EnduRen Live Cell Substrate provides new capabilities in
performing luminescent reporter assays by enabling live cell kinetic measurements,
streamlining assay development and multiplexing with other lytic
assays. EnduRen Live Cell Substrate provides the ability to measure Renilla
luciferase luminescence for at least 24 hours after substrate addition, with up
to tenfold higher signal-to-background ratios than wildtype coelenterazines.
EnduRen Live Cell Substrate is a uniquely engineered coelenterazine with
protected oxidation sites, which minimizes substrate degradation and
autoluminescence (background) in cell culture, while it extends the luminescent
signal to accommodate microplates without the need for auto-injectors. The
result is that EnduRen Live Cell Substrate overcomes the key limitations of
wildtype coelenterazines by providing an automation-friendly, highly sensitive
substrate for Renilla luciferase-based gene reporter and BRET applications.
Features:
• Live Cell Assay: Generate kinetic profiles for reporter gene, BRET and
RNAi applications.
• Kinetic Reporter Gene Analysis: Conserve test compounds as you
create response profiles in real time to generate more data-rich results.
• Streamlined Assay Development and Screening: Rapidly obtain
optimal assay parameters through repeat measurements using only a
single cell population. Increase your sample throughput using microplates
without time-consuming per-sample reagent injection steps.
• Designed for Multiplexing: Perform more dynamic experiments using
the same sample set by pairing with any lytic assay.
• High Signal-to-Background Ratios: Reliably quantitate low levels of
expression for reporter gene detection and BRET.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
Protocol
EnduRen Live Cell Substrate Technical Manual
Isoproterenol
(6µM)
Fold Induction
12
10
8
6
4
2
Forskolin
(10µM)
0
0 6 12 18 24 30 36
Time (hours)
Part#
TM244
4273MA08_3A
Section
Contents
Real-Time live cell reporter assay using the EnduRen Substrate.
Luminescence was monitored from HEK 293 cells for >24 hours,
permitting measurement of the effects of sequential treatment of the cells
with isoproterenol and forskolin.
Table of
Contents
304
For complete and up-to-date product information visit: www.promega.com/catalog