2013 Promega catalogue

Cell Signaling
MagneHis Protein Purification System
Product Size Cat.# Price ($)
MagneHis Protein Purification System 65 reactions V8500 303
325 reactions V8550 1078
Available Separately Size Cat.# Price ($)
MagneHis Ni-Particles 2 ml V8560 236
For Research Use Only. Not for Use in Diagnostic Procedures.
10 ml V8565 832
Description: The MagneHis Protein Purification System provides a simple,
rapid and reliable method for the purification of polyhistidine- or HQ-tagged,
expressed proteins. Paramagnetic precharged nickel particles (MagneHis
Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly
from a crude cell lysate using either a manual (requires use of a magnetic
stand) or automated procedure. Using a tube format, polyhistidine- or
HQ-tagged protein can be purified on a small scale using less than 1ml of
culture or on a large scale using more than 1 liter of culture. Samples can be
processed in a high-throughput manner using a robotic platform such as the
Beckman Coulter Biomek ® 2000 or FX.
Features:
• Simple: No centrifugation or vacuum is required once the cells are lysed.
• Flexible: MagneHis Ni-Particles are compatible with a variety of
common buffers.
• Quick: No long incubations with lysozyme are required for cell lysis.
• Scalable: Volumes can be adjusted to correspond to the amount of
material to be purified, 1ml to 1 liter of cell culture.
• Efficient: Binding capacity is up to 1mg of polyhistidine-tagged protein
per 1ml of MagneHis Ni-Particles.
• Versatile: Purification can be performed manually (requires use of a
magnetic stand) or using an automated platform.
• Convenient: Complete system that includes all necessary components,
including a unique lysis buffer.
• Automate This Assay: Validated automated methods available for
Beckman Coulter Biomek ® 2000 and FX and Tecan Genesis ® RSP at:
www.promega.com/automethods/
Storage Conditions: Store at 4°C.
Protocol
MagneHis Protein Purification System Technical Manual
MagZ Protein Purification System
Part#
TM060
Product Size Cat.# Price ($)
MagZ Protein Purification System 30 reactions V8830 376
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The MagZ Protein Purification System provides a simple, rapid
and reliable method for the purification of expressed polyhistidine- or HQtagged
proteins, which are 99% free of hemoglobin contamination, from rabbit
reticulocyte lysate. Based on the use of proprietary, paramagnetic precharged
particles, polyhistidine- or HQ-tagged protein can be isolated from 50–500μl of
TnT ® Coupled Transcription/Translation reactions. Polyhistidine- or HQ-tagged
proteins bind to the particles in minutes, while unbound proteins are washed
away, and the target protein is eluted with imidazole.
Features:
• Specific: Minimal hemoglobin (20mmol Ni/ml
settled resin). The resin is designed to efficiently capture and purify bacterially
expressed polyhistidine- or HQ-tagged proteins. The HisLink Resin also
may be used for general applications requiring an immobilized metal affinity
chromatography (IMAC) matrix. HisLink Resin may be used in either column
or batch purification formats. The lysate flows by gravity over a column of
packed HisLink Resin at a rate sufficient for complete capture and efficient
elution of polyhistidine- or HQ-tagged proteins from cleared lysate. The resin
may be used in vacuum filtration devices (e.g., Vac-Man ® Vacuum Manifold
[Cat.# A7231]), allowing simultaneous processing of multiple columns.
The HisLink Resin is also an excellent choice for affinity purification of
polyhistidine- or HQ-tagged proteins with low- to medium-pressure liquid
chromatography systems such as FPLC. In batch format, HisLink Resin may
be separated easily from the lysate without filtration (or centrifugation), allowing
processing of larger quantities of lysate and the ability to purify protein without
clearing the lysate of insoluble cellular debris.
Features:
• Optimize Yields: High binding capacity (>15mg/ml).
• Save Time: Purify polyhistidine- or HQ-tagged proteins from cleared or
crude cell lysates.
• Flexible: Use standard gravity column chromatography or automated
applications such as FPLC.
Storage Conditions: Store at 4°C.
Protocol
HisLink Protein Purification Resin Technical Bulletin
HisLink 96 Protein Purification System
Part#
TB327
Product Size Cat.# Price ($)
HisLink 96 Protein Purification System 1 × 96 blank V3680 472
5 × 96 blank V3681 1989
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: HisLink 96 Protein Purification System provides a simple,
quick and robust method of purifying multiple polyhistidine- or HQ-tagged
expressed proteins from E. coli using a vacuum-based method. The system is
designed to purify expressed polyhistidine- or HQ-tagged proteins directly from
deep-well, 96-well plates. The HisLink 96 System is amenable to manual
or automated methods, such as the Beckman Coulter Biomek ® 2000 or FX for
high-throughput applications.
Using the provided FastBreak Cell Lysis Reagent, bacterial cells containing
polyhistidine- or HQ-tagged protein are lysed directly in culture. The HisLink
Resin is added directly to the lysate, and the polyhistidine- or HQ-tagged
proteins bind in a matter of minutes. Samples are then transferred to a filtration
plate, unbound proteins are washed away and the target protein is recovered
by elution with imidazole.
Features:
• Simple: No centrifugation required; lysis buffer is added directly to cells in
culture medium.
• Quick: No long lysozyme incubations are required for cell lysis.
• Versatile: Perform purification manually or on an automated platform.
• Efficient: Binding capacity of 1mg of polyhistidine-tagged protein per well.
Storage Conditions: Store the system at 4°C. Plates may be stored at 4°C or
room temperature.
Protocol
HisLink 96 Protein Purification System Technical Bulletin
Part#
TB342
16
Protein Purification and Interactions
Section
Contents
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