2013 Promega catalogue

Cell Signaling
Riboprobe ® System Components and Buffers
Product Size Conc. Cat.# Price ($)
Riboprobe ® System Buffers 1 system P1121 146
rATP, rCTP, rGTP, rUTP, each at 10mM in 0.5 ml 10 mM P1221 187
separate tubes
Available Separately Size Conc. Cat.# Price ($)
RQ1 RNase-Free DNase 1,000 u 1 u/µl M6101 86
rATP, 10mM 0.5 ml 10 mM P1132 69
rCTP, 10mM 0.5 ml 10 mM P1142 69
rGTP, 10mM 0.5 ml 10 mM P1152 69
rUTP, 10mM 0.5 ml 10 mM P1162 69
DTT, Molecular Grade 100 μl 100 mM P1171 47
Transcription Optimized 5X Buffer 200 μl P1181 47
Nuclease-Free Water 50 ml P1193 70
For Laboratory Use.
Description: Riboprobe ® System Buffers are components of the single and
combination Riboprobe ® Systems. The buffers are also available as standalone
products.
RQ1 RNase-Free DNase is used to remove template DNA from RNA
preparations and is qualified for use in applications where maintaining the
integrity of RNA is critical. Product is quality tested to ensure the absence of
detectable RNase activity. 10X Reaction Buffer and 10X Stop Buffer included.
rATP, rCTP, rGTP and rUTP are provided in individual tubes, qualified for use
with the Riboprobe ® Systems. The rNTPs are supplied in nuclease-free water.
Purity has been verified by HPLC analysis.
Features:
• Pretested: Reagents are tested with other Riboprobe ® System components.
rNTPs are tested for functionality with in vitro transcription reactions.
• Transcription Qualified: Reagents are qualified for use for in vitro
transcription reactions with SP6, T7 or T3 RNA Polymerase.
Storage Conditions: Store at –20°C.
Protocol
RQ1 RNase-Free DNase Product Information
Riboprobe ® In Vitro Transcription Systems Technical Manual
Part#
9PIM610
TM016
Ribo m 7 G Cap Analog
Product Size Conc. Cat.# Price ($)
Ribo m 7 G Cap Analog 10 A 254 units 40 mM P1711 354
25 A 254 units 40 mM P1712 673
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Ribo m 7 G Cap Analog is a modified ribonucleotide with the
structure (m 7 G(5´)ppp(5´)G). This methylated ribonucleotide can be incorporated
onto the 5´-end of transcripts synthesized in vitro and simulates the 7-methyl
guanosine 5´-cap structure found on most eukaryotic mRNA molecules.
Features:
• Improved Translation: Enhances translation efficiency in many
reticulocyte-based reactions.
• Effective: Protects RNA from intracellular digestion.
• Flexible: Can be used in either the Riboprobe ® Systems or RiboMAX
Large Scale RNA Production Systems.
Storage Conditions: Store at –20°C.
Protocol
Ribo m 7 G Cap Analog Protocol
pGEM ® Express Positive Control Template
Part#
9PIP171
Product Size Cat.# Price ($)
pGEM ® Express Positive Control Template 10 μg P2561 85
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The pGEM ® Express Positive Control Template is created by
linearizing a vector with the restriction enzyme ScaI. The Positive Control
Template may be used to monitor in vitro transcription reactions when using
the Riboprobe ® Systems.
Features:
• Multi-Sized RNAs: SP6 RNA polymerase produces transcripts of 1,787
and 2,566 bases; T7 RNA polymerase produces transcripts of 1,065 and
2,346 bases; T3 RNA Polymerase produces transcripts of 250 and 1,525
bases.
• Flexible: Template can be used with SP6, T7 or T3 RNA polymerases.
Storage Conditions: Store at –20°C.
18
RNA Analysis
Protocol
pGEM ® Express Positive Control Template Product Information
Part#
9PIP256
Section
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