2013 Promega catalogue
2013 Promega catalogue
2013 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2013</strong><br />
Worldwide Contact List<br />
Cell Signaling<br />
pGEM ® -4Z Vector<br />
Product Size Cat.# Price ($)<br />
pGEM ® -4Z Vector 20 μg P2161 143<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The pGEM ® -4Z Vector is intended for use as a standard cloning<br />
vector, as well as for the highly efficient synthesis of RNA in vitro. The vector<br />
carries the lacZ α-peptide and the multiple cloning region arrangement from<br />
pUC18 allowing selection of recombinants by blue/white screening. In addition,<br />
the vector contains both the SP6 and T7 RNA polymerase promoters flanking<br />
the multiple cloning region.<br />
The pGEM ® -3Z and pGEM ® -4Z Vectors are essentially identical except for the<br />
orientation of the SP6 and T7 promoters.<br />
Features:<br />
• Blue/White Screening: Allows the easy identification of recombinant<br />
clones.<br />
• Versatile: This vector can be used for standard cloning and in vitro transcription<br />
from SP6 and T7 RNA polymerase promoters flanking the multiple<br />
cloning region.<br />
• Convenient: Multiple cloning site provides a selection of restriction sites<br />
for cloning.<br />
Storage Conditions: Store vector at –20°C and bacterial strain at –70°C.<br />
Protocol<br />
pGEM ® -4Z Vector Technical Bulletin<br />
XmnI 1939 SspI 2144<br />
ScaI 1820 AatII 2262<br />
Amp r<br />
pGEM ® -4Z<br />
Vector<br />
(2,746bp)<br />
ori<br />
lacZ<br />
NdeI<br />
2511<br />
NarI<br />
2563<br />
SP6<br />
EcoRI<br />
SacI<br />
KpnI<br />
AvaI<br />
XmaI<br />
SmaI<br />
BamHI<br />
XbaI<br />
SalI<br />
AccI<br />
HincII<br />
PstI<br />
SphI<br />
HindIII<br />
➞<br />
➞<br />
T7<br />
1 start<br />
7<br />
17<br />
23<br />
23<br />
23<br />
25<br />
28<br />
34<br />
40<br />
41<br />
42<br />
50<br />
56<br />
58<br />
70<br />
Part#<br />
TB036<br />
0280VA05_4A<br />
pGEM ® -5Zf(+) Vector<br />
Product Size Cat.# Price ($)<br />
pGEM ® -5Zf(+) Vector 20 μg P2241 158<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The pGEM ® -5Zf(+) Vector is derived from the pGEM ® -3Zf(+)<br />
Vector and contains the origin of replication of the filamentous phage f1. This<br />
plasmid serves as a standard cloning vector, as a template for in vitro transcription<br />
and can be used for the production of circular ssDNA. This vector contains<br />
T7 and SP6 RNA polymerase promoters flanking a multiple cloning region<br />
within the α-peptide coding region of β-galactosidase. Insertional inactivation<br />
of the α-peptide allows recombinant clones to be identified directly by color<br />
screening on indicator plates when using appropriate E. coli strains (e.g.,<br />
JM109). The multiple cloning region contains unique restriction sites for ApaI,<br />
AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI.<br />
This arrangement is designed specifically for generating unidirectional deletions<br />
with the Erase-a-Base System.<br />
Features:<br />
• Blue/White Screening: Allows the easy identification of recombinant<br />
clones.<br />
• Versatile: This vector can be used for standard cloning, single-stranded<br />
DNA production and in vitro transcription from SP6 and T7 RNA polymerase<br />
promoters flanking the multiple cloning region.<br />
• Convenient: Multiple cloning site provides a selection of restriction sites<br />
for cloning.<br />
• Unidirectional Deletions: Restriction sites are positioned conveniently for<br />
use with the Erase-a-Base System.<br />
Storage Conditions: Store vector at –20°C and bacterial strain at –70°C.<br />
Protocol<br />
pGEM ® -5Zf(+) Vector Technical Bulletin<br />
XmnI 1994<br />
ScaI<br />
1875<br />
Amp r<br />
pGEM ® -5Zf(+/–)<br />
Vectors<br />
(3,000bp)<br />
ori<br />
f1 ori<br />
lacZ<br />
T7<br />
➞<br />
ApaI<br />
AatII<br />
SphI<br />
NcoI<br />
SacII<br />
EcoRV<br />
SpeI<br />
NotI<br />
PstI<br />
SalI<br />
NdeI<br />
SacI<br />
BstXI<br />
NsiI<br />
➞<br />
SP6<br />
Part#<br />
TB047<br />
1 start<br />
14<br />
20<br />
26<br />
37<br />
46<br />
51<br />
55<br />
62<br />
73<br />
75<br />
82<br />
94<br />
103<br />
112<br />
126<br />
0284VA05_4A<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
116<br />
For complete and up-to-date product information visit: www.promega.com/catalog
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