2013 Promega catalogue

Life
Science
Catalog
2013
Worldwide Contact List
Cell Signaling
Wizard ® Plus Maxipreps DNA Purification
System
Product Size Cat.# Price ($)
Wizard ® Plus Maxipreps DNA Purification System 10 preps A7270 307
Available Separately Size Cat.# Price ($)
Cell Resuspension Solution (CRA) 150 ml A7112 75
Cell Lysis Solution (CLA) 150 ml A7122 75
Neutralization Solution (NSA) 150 ml A7131 106
Column Wash Solution (CWB) 125 ml A8102 88
Wizard ® Maxipreps DNA Purification Resin 500 ml A7401 685
Wizard ® Maxi/Megapreps Filtering System 50 each A7421 601
A7112, A7122 For Laboratory Use. A7270, A7131, A8102, A7401, A7421 For Research Use
Only. Not for Use in Diagnostic Procedures.
Description: The Wizard ® Plus Maxipreps DNA Purification System provides a
simple and rapid resin-based batch column method for purification of plasmid
DNA that eliminates the need for cesium chloride:ethidium bromide gradient
centrifugation. Use of this system requires only a centrifuge, a vacuum source
and a vacuum manifold. This system typically yields 300μg–1mg of high-copynumber
plasmid DNA (200–20,000bp) from a 100–500ml culture in less than
3 hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) and
can be used directly for DNA sequencing and restriction digestion without
further manipulation. The DNA also can be used for in vitro transcription
reactions supplemented with a ribonuclease inhibitor such as Recombinant
RNasin ® Ribonuclease Inhibitor (Cat.# N2511).
Features:
• Flexible: DNA is suitable for restriction enzyme digestions, automated
fluorescent DNA sequencing, transformation and subcloning.
• High Quality: Yields plasmid DNA of comparable quantity and quality to
cesium chloride:ethidium bromide gradient techniques that are much more
time- and labor-intensive.
• Fast: Rapid batch binding and column washing method used for DNA
isolation.
• Safe: Eliminates the need for cesium chloride:ethidium bromide gradient
centrifugation and does not require organic extractions.
Storage Conditions: Store at 22–25°C.
Protocol
Wizard ® Plus Maxipreps DNA Purification System Technical Bulletin
Part#
TB139
Wizard ® Plus Megapreps DNA Purification
System
Product Size Cat.# Price ($)
Wizard ® Plus Megapreps DNA Purification System 5 preps A7300 307
Available Separately Size Cat.# Price ($)
Cell Resuspension Solution (CRA) 150 ml A7112 75
Cell Lysis Solution (CLA) 150 ml A7122 75
Neutralization Solution (NSA) 150 ml A7131 106
Column Wash Solution (CWB) 125 ml A8102 88
Wizard ® Megapreps DNA Purification Resin 1,000 ml A7361 1241
Wizard ® Maxi/Megapreps Filtering System 50 each A7421 601
A7112, A7122 For Laboratory Use. A7300, A7131, A8102, A7361, A7421 For Research Use
Only. Not for Use in Diagnostic Procedures.
Description: Wizard ® Plus Megapreps DNA Purification System provides a
simple and rapid method for large-scale purifications of plasmid DNA that
eliminates the need for cesium chloride:ethidium bromide gradient
centrifugation. Use of this system requires only a centrifuge, a vacuum source
and a vacuum manifold. The system yields greater than one milligram of highcopy-number
plasmid DNA (200–20,000bp) from a 1,000ml culture in less
than 3 hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193)
or TE buffer and can be used directly for DNA sequencing and
restriction digestion without further manipulation. The DNA also can be used for
in vitro transcription reactions supplemented with a ribonuclease inhibitor such
as Recombinant RNasin ® Ribonuclease Inhibitor (Cat.# N2511).
Features:
• Fast: Rapid batch binding and column washing method used for DNA
isolation
• Safe: Eliminates the need for cesium chloride:ethidium bromide gradient
centrifugation and does not require organic extractions.
• Reliable: Yields plasmid DNA of comparable quantity and quality to cesium
chloride:ethidium bromide gradient techniques that are much more timeand
labor-intensive.
• Yield: Each megaprep produces >1mg of DNA from 1,000ml of bacterial
culture when using a high-copy-number plasmid.
• Quality: DNA is suitable for restriction enzyme digestions, automated
fluorescent DNA sequencing, transformation and subcloning.
Storage Conditions: Store at 22–25°C.
Protocol
Wizard ® Plus Megapreps DNA Purification System Technical Bulletin
Part#
TB140
Section
Contents
Table of
Contents
138
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